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3Chondrogenesis was assessed using Alcian blue staining, Toluidine bluestaining, Safranin O/Fast Green staining at 7 and 14 days, still collagenⅡimmunohistochemistry and aggrecan immunofluorescence at 4, 7 and14 days after initial chondrogenic induction of adipose -derivedmesenchymal stem cells.

3观察脂肪间充质干细胞在诱导后的生长增殖情况、形态变化,于诱导7、14天后应用阿尔新兰染色、甲苯胺兰染色、藩红0/固绿染色,以及4、7、14天后应用Ⅱ型胶原蛋白免疫细胞化学、aggrecan免疫荧光评价其成软骨能力。

The extracellular matrix wasstained positively for Alcian blue, Toluidine blue and Safranin O / FastGreen at 7 and 14 days after initial chondrogenic induction of adipose -derived mesenchymal stem cells, and indicated collagenⅡand aggrecanwere expressed positively.

2脂肪间充质干细胞在高密度的"微团"培养条件下,于诱导7、14天后阿尔新兰染色、甲苯胺兰染色、藩红0/固绿染色阳性,诱导4、7、14天后Ⅱ型胶原蛋白免疫细胞化学、aggrecan免疫荧光阳性表达。

Objective To explore whether the toxic effects on the primary cultured astroglia would be induced by different arsenicals.

目的 观察不同砷化物对原代培养星型胶质细胞的毒性作用。

Methods:The cattle cancellus bone were degreased,deproteined and calcined to form TBC vector.After the surface was covered with collagen.the cultured rabbit osteoblasts were compounded to TBC in vitro.5,10 and 20 day later,the compound materials were observed under scanning electron microscorpe.

牛松质骨经脱脂、脱蛋白、煅烧等工艺制成煅烧骨作为载体,载体表面经胶原处理后将体外培养的兔骨膜成骨细胞复合到TBC,分别于5日,10日和20日取材,行扫描电镜观察,设对照组。

I suspect this is a case of benign schwannoma, with characteristic spindled cells between collagenous stroma.

我猜想这例为良性神经鞘瘤,在胶原基质间为特征性的梭型细胞。

Methods: Isolated rat costalchondral growth plate by microdissection, dispersed RGC (rat costochondral growth plate chondrocyte) were obtained by digestion (0.05% hyaluronidase and 0.2% collagenase type Ⅱ), inoculated and cultured in monolayer....

显微解剖分离出大鼠肋生长板,酶消化法(0.05%透明质酸酶和0.2%Ⅱ型胶原酶)获得分散的大鼠肋生长板软骨细胞(rat costochondral growth plate chondrocyte,RGC),常规接种,单层培养。

Purpose To study the role of pulmonary macrophages in the collagen deposition of fibrotic interstital lung disease.

目的:探讨肺泡巨噬细胞在纤维性间质性肺病胶原纤维异常沉积中的作用。

At the 8 week postoperatively,three groups still have not restored the normal thichness of corneal epithelial cell;but in the blank group there was more neoformative irregular collagen in the anterior stromal layer.

术后4周,三组覆盖切削区的角膜上皮细胞仍有轻度增生,空白组前基质层内较多新生胶原,排列不规则,失去板层结构,瘢痕形成。

Fig 3Lots of rough endoplasmic reticula, mitochondrid an d collagen in the cytoplasm of fibroblast in control group

图3对照组的瘢痕成纤维细胞粗面内质网、线粒体丰富,形态结构正常,周围胶原纤维较多TEM×5200

After observing the specimens from JMA-m groups, we have found that epithelium and endothelium of cornea full, the lesion are light edema at stromatic layer. One small amount of inflammatory-cell infiltrate presents the collagen fiber lack of rules at shallow stromatic layer.

观察角膜安的结果表明,中药中剂量组角膜上皮和内皮层完整,基质轻度水肿,浅基质层内少量炎性细胞浸润,胶原纤维走行欠规则,整体改变较低剂量组减轻。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?