细胞原质的

Methods We isolated and cultured the MSCs, then induced MSCs into osteoblast and checked the osteoblast by RT-PCR; the conditioned medium in different concentration was prepared containing diphosphonate or assemble flavone of drynaria rhizome; with the conditioned medium the osteoblast was cultured; then the growth curve was analyzed and the cytogene (collagenase col Ⅰ) col Ⅰ and ALP expression was detected.

使用诱导方法作用于骨髓基质干细胞，使之转化为成骨细胞；用RT-PCR检验成骨细胞；不同浓度的二磷酸盐或骨碎补总黄酮配制成条件培养基，作用于成骨细胞，并分析生长曲线，分析细胞基因碱性磷酸酶和胶原酶Ⅰ(collagenase col Ⅰ)表达情况。

Then test of film cell growth by high sugar, LPS, high sugar plus LPS was done with the result showing that FANG KIDNEY capsule can restrain the growth of film cell and composition of external base, it can be concluded that it has obvious effect in preventing the hardening of kidney small cell.

为进一步探讨糖肾胶囊的作用机制，还采用国内外经典细胞培养方法，成功地培养出肾小球系膜细胞，在此基础上进行高糖、LPS、及高糖加LPS诱导细胞膜增生，结果发现糖肾胶囊能抑制系膜细胞增生及细胞外基质胶原合成，证明其对防止肾小球硬化有明显作用。

Methods:(1) Bone marrow stromal cells isolated from Beagle dogs were cultured in vitro for the primary culture,in basic medium consisted ofDMEM^iew horning cattleerum,etc. Parts of subculture were cultured in mineralization medium which additionally contained 50 y g/ml ascorbic acid, 10mmol/LNa- beta -Glycerophosphate and 10-8mol/L dexamethasone in order to induce cells differentiating into osteoblasts.

①抽取毕格犬髂骨骨髓体外分离培养获得骨髓基质细胞，DMEM、新生牛血清培养基进行原代培养，部分传代细胞以含10~(-8)mol/L地塞米松、50μg/ml抗坏血酸和10mmol/Lβ—甘油磷酸钠的矿化培养液诱导其向成骨细胞增殖分化，用倒置相差显微镜进行细胞形态学和细胞生长增殖观察。

Methods Primary metanephric mesenchyme stem cells were cultivated and multi-directional differentiation was induced.

方法原代分离培养后肾间充质干细胞，在体外特定诱导条件下观察细胞向成骨细胞、脂肪细胞及肾小管上皮细胞的分化及其形态演变，采用细胞免疫荧光检测细胞表面特定蛋白的表达变化。

The microstructure of the scaffold was observed under photomicroscope and scanning electron microscopy,and the in vitro degradation time,permeability,tensile-strength,porosity,shrinka...

实验结果显示：支架中的成纤维细胞、脂肪细胞及组织纤维间质完全去除，胶原纤维得到了松散，并维持其原有的天然三维网络多孔结构；该材料透水汽性处于3000g.m-2.d-1左右，适合创面恢复；体外降解时间处于25～50h之间，并可根据需要调整工艺条件控制降解时间；拉伸强度介于10.20～11.50MPa之间，具有良好的拉伸强度；收缩温度介于70～85℃之间。上述结果表明该材料已解决了猪脱细胞真皮基质渗透性差、降解速度过慢的缺点，并且其透气性和拉伸强度高、降解性优良且可控，符合组织工程支架材料的要求。

According to the changes of the structure of chromatin and the morphology of cell, the process of the typical spermatogenesis of Cipangopaludina chinensis can be divided into five stages: spermatogonium, primary spermatocyte, secondary spermatocyte, spermatid and mature sperm.

中国圆田螺典型精子发生时，根据染色质和细胞形态的变化，可将其发生分为以下五个时期：精原细胞、初级精母细胞、次级精母细胞、精细胞和成熟精子。

METHODS AND RESULTS: Using both primary peritoneal macrophages and studies in advanced atheromata in vivo, we introduce signal transducer and activator of transcription-1 (STAT1) as a critical and necessary component of endoplasmic reticulum stress/type A scavenger receptor-induced macrophage apoptosis.

方法和结果：通过使用原代腹膜巨噬细胞研究在体脂质斑块，我们引入信号转导子和转录激活子-1(STAT1)，作为内质网应激/A型清道夫受体诱导的巨噬细胞凋亡中关键和必要组分。

It starts transmitting information by integrating with lagen and stromatin by irritating osteocytes and chondrocytes to form rapidly extracellular matrix so as to promote repair of bone and cartilage injury.

转化生长因子β通过刺激骨、软骨细胞合成胶原和基质蛋白，快速形成细胞外基质，达到其促进骨、软骨损伤修复的作用。

Objective To investigate the potential pathogenicity of Mycoplasma penetrans (M. penetrans), and to study nitric oxide production and cell prolification in lipid-associated membrane proteins-stimulated mouse macrophages.

目的 检测穿透支原体脂质相关膜蛋白能否诱导小鼠巨噬细胞产生一氧化氮及其对细胞增殖的影响，以了解穿透支原体潜在的致病性。

The findings of the newly identified goose paramyxovirus strain GPMV/HBexperimental infection were as follows: The challenge caused high morbidity and highmortality.The clinical signs included severe depression, decreased appetite or anorexia,white,yellow and green diarrhea, with neural symptoms;pathological changes showed thathaemorrhage and ulceration of intestine mucosa, necrosis of spleen and pancreas,andedema of liver and kidney, uratic deposition in kidney.Eosinophilic inclusion bodies werefound in the cytoplasm of pancreas and a large quantities of fiber protein were found inintestine. Lots of hemosidefin was found in some area of liver and kidney. A number ofT、B lymphocytes reduced sharply in spleen,lymph nodes in dead geese. Transmissionalelectron microscope showed that some cells had the morphological characteristics ofmitochondria swelling, morphological changes were condensation of nuclear chromatininto dense masses,followed by its marginating against the nuclear envelope, andespecially concentration of cytoplasm association with lots of lipid droplet.

人工感染雏鹅实验中，雏鹅临床表现为高发病率和高死亡率，食欲和饮水减少，拉白色、黄绿色稀粪，伴有神经症状；常规组织学观察，脾脏和胰腺肿大，有白色坏死斑点，肠道出血和坏死，肝脏肿大变性，肾脏肿大变性有尿酸盐沉着等；用特殊染色和组织化学方法首次对人工感染鹅副粘病毒的雏鹅的重要脏器进行研究，结果表明：胰腺中发现病毒包涵体，肠道中大量纤维素以及胶原纤维增生，肾脏、肺脏大量含铁血黄素沉着，免疫器官如脾脏和淋巴结中T、B淋巴细胞大量减少；电镜下多种细胞呈现线粒体水肿，多种实质细胞的染色质固缩，染色质边集，特别是细胞内容物如脂肪滴增多的病理变化特征。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。