细胞化学

OVCs were isolated by density ladder centrifugation in the 4th week, and then OVC's morphology was observed under transmission electromicroscrope and immunocytochemistry were performed to detect the expression of ICAM-1. Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast. In initial stage of damaged liver, OVCs and the expression of ICAM-1 mostly distributed around Hering duct, and then gradually increased and expanded toward hepatic lobule, shown by staining paraffin sections with HE, immunochemistry and transmission electromicroscrope. Immunocytochemistry indicated that ICAM-1 expression on OVCs was positive.

结果 透射电镜下观察到OVCs超微结构的改变为核大，核/浆比大，核仁小，胞浆内细胞器少，可见少量内质网和小线粒体，整个细胞呈幼稚、未分化状态；ICAM-1免疫细胞化学检测阳性；损伤肝组织HE染色和ICAM-1免疫组化检测显示，肝损伤初期，Hering管周围OVCs增生并且ICAM-1阳性表达，随着肝损伤的加重，OVCs继续增生，并且，OVCs沿增生的肝纤维向肝小叶迁移，同时，ICAM-1阳性表达也继续增多，并逐渐向肝小叶弥散分布；透射电镜下观察到，肝损伤初期，OVCs在Hering管周围增生，肝纤维随之增生，增生的OVCs与肝纤维黏附生长。

Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast. In initial stage of damaged liver, OVCs and the expression of ICAM-1 mostly distributed around Hering duct, and then gradually increased and expanded toward hepatic lobule, shown by staining paraffin sections with HE, immunochemistry and transmission electromicroscrope. Immunocytochemistry indicated that ICAM-1 expression on OVCs was positive.

结果 透射电镜下观察到OVCs超微结构的改变为核大，核/浆比大，核仁小，胞浆内细胞器少，可见少量内质网和小线粒体，整个细胞呈幼稚、未分化状态；ICAM-1免疫细胞化学检测阳性；损伤肝组织HE染色和ICAM-1免疫组化检测显示，肝损伤初期，Hering管周围OVCs增生并且ICAM-1阳性表达，随着肝损伤的加重，OVCs继续增生，并且，OVCs沿增生的肝纤维向肝小叶迁移，同时，ICAM-1阳性表达也继续增多，并逐渐向肝小叶弥散分布；透射电镜下观察到，肝损伤初期，OVCs在Hering管周围增生，肝纤维随之增生，增生的OVCs与肝纤维黏附生长。

Immunocytochemical staining was conducted for the neural stem cells, neuron and neuroglia specific cell surface marker such as nestin, MAP-2, NSE and GFAP.

免疫细胞化学方法检测诱导前后神经前体细胞、神经元和神经胶质细胞特异标记物如nestin，MAP-2，NSE和GFAP的表达。

Methods: The effects of Opuntia on proliferation of fibroblast were measured with MTT colorimetric assay, and PCNA productions were examined with immunocytechemistry.

以小鼠成纤维细胞株(NIH3T3)为研究对象，采用四氮唑盐比色法测定仙人掌对成纤维细胞增生的影响，采用免疫细胞化学染色法检测成纤维细胞的增殖细胞核抗原的表达。

The blank group, the pure chondrogenic inductor group and the group of the G ranula of P enetrating Bone and Removing Pain mixed with chondrogenic inductor. We adopted pro-culture solution, pure chondrogenic induced culture solution （ TGF-β310ug/L , Dex10-7mol/L , VitC50mg/L ） and the chondrogenic induced culture solution which included the serum of the G ranula. All groups were cultivated in 50ml cell culture bottles. The effects of the G ranula of P enetrating Bone and Removing Pain on chondrogenic phenotype differentiation of BMSCs were investigated after being cultivated for 1, 2, 3 weeks, then cells observed by inverted phase contrast microscope and immunocytochemical stain.

3将第2代SD大鼠BMSCs分为空白对照组、单纯软骨诱导剂组及透骨消痛颗粒含药血清加软骨诱导剂组，采用原培养液、软骨诱导培养液（TGF-β310ug/L，Dex10-7mol/L，VitC50mg/L）及透骨消痛颗粒含药血清的软骨诱导培养液，50ml细胞培养瓶内进行培养，1、2、3周后通过倒置相差显微镜及免疫细胞化学染色等实验技术，研究透骨消痛颗粒对BMSCs诱导成软骨细胞的影响。

Methods The different expression of CK in SHEE and SHEEmt cell lines,was observed by the immunocytochemistry and Western blotting.

采用免疫细胞化学染色和免疫印迹的方法，观察SHEE细胞和SHEEmt细胞中CK的表达。

Methods: After stimulation with different concentrations of silicon dioxide, the morphological changes of the above macrophages were observed and the TNF-α expression was detected by immunocytochemistry as well.

用不同浓度的SiO2刺激巨噬细胞后，观察其细胞的形态学改变，并用免疫细胞化学检测其TNF-α的蛋白表达。

The morphological changes of VEC in each group were observed by the inverted microscope, and the expression of ICAM-1 in each group was detected by immunocytochemistry.

通过倒置相差显微镜观察和比较，各组内皮细胞在形态上的变化，用免疫细胞化学染色方法检测ICAM-1在各组内皮细胞中的表达。

In the present study,in order to explore the roles of estrogen and progestin in epilepsy and elucidate their mechanisms of action, we utilized animal seizure models induced by intracerebroventricular coriaria lactone and intraperitoneal bemegride, studied the effects of estrogen and progestin on central nervous system functions in behavior,electrophysiological,cellular,molecular and gene levels by means of neuroelectrophysiology, flow cytometry, high performance liquid chromatography, immunocytochemical and in situ hybridization techniques.

为了探索雌、孕激素在癫痫发病中的作用，阐明其作用机制，本工作分别以马桑内酯侧脑室注射致痫、贝美格（Be腹腔注射致痫大鼠为实验对象，采用神经贫血电生理、流式细胞美白免疫荧光、高效液相色谱、美白免疫细胞化学、原位杂交早泻技术，从整体、行为、细胞、分子以及基因水平研究发现了雌、孕激素对大鼠中枢神经贫血系统功能的影响。

Methods: rat costochondral growth plate chondrocytes were isolated and cultured. growth kinetics of rgcs during serial passage was analyzed, and α sma, collagen typeⅱ and gag expressions of primary to 5th passage rgcs were detected by immunocytochemistry, western blot and alcian blue staining, respectively.

分离、培养大鼠肋生长板软骨细胞，对原代至第5代rgcs进行细胞生长动力学分析，阿尔新兰染色、免疫细胞化学法和蛋白质印迹分别检测蛋白多糖，α sma和ⅱ型胶原的表达。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。