细胞化学

METHODS: Lipofectamine method: The BMMSCs were obtained from the tibias and femurs of the guinea pigs. The third passage BMMSCs were cultured with plasmid-lipofectamine mixture for 6 hours, followed by fetal bovine medium for 48 hours. Immunohistochemistry was performed for transient expression. G418 was added after 48 hours.

①脂质体法：取体外分离、培养的第3代豚鼠骨髓间充质干细胞，将质粒-脂质体混合物加入含细胞的培养基中培养6 h，再加入胎牛血清的培养基，孵育48 h 后行免疫组织化学检测，即为瞬时表达。48 h后加入含G418培养基筛选。

The purpose of this reviewis to present a structured assessment of the anti-tumour and cytotoxic properties of 150 marine natural products, many of which are novel compounds that belong to diverse structural classes, including polyketides, terpenes, steroids and peptides.

本综述旨在对大约150个海洋天然产物的抗肿瘤及细胞毒活性进行化学结构上的分析，其中许多化合物属于不同的结构类型，包括聚酮化合物、帖类、甾体以及多肽。

The purpose of this reiewis to present a structured assessment of the anti-tumour and cytotoxic properties of 150 marine natural products, many of which are noel compounds that belong to dierse structural classes, including polyketides, terpenes, steroids and peptides.

本综述旨在对大约150个海洋天然产物的抗肿瘤及细胞毒活性进行化学结构上的分析，其中许多化合物属于不同的结构类型，包括聚酮化合物、帖类、甾体以及多肽。

Methods After immunohistochemical staining, light microscope was employed to determine expressions of VEGF and TAMs. Statistical analysis was performed by using SPSS software.

采用免疫组织化学染色，在光镜下对VEGF阳性细胞评分及TAMs计数进行分析。

Methods after immunohistochemical staining,light microscope was employed to determine expressions of vegf and tams. statistical analysis was performed by using spss software.

采用t型淋巴癌是什么组织化学染色，在光镜下对vegf阳性细胞评分及tams计数进行分析。

Methods after immunohistochemical staining,light microscope was employed to determine expressions of vegf and tams. statistical analysis was performed by using spss software.

采用免疫宫颈腺癌em化学染色，在光镜下对vegf阳性细胞评分及tams计数进行分析。

Biotechnology Research Institute is primarily dedicated to education and research in biotechnology including molecular bio-pharming, subviral RNA pathogens, the metabolism of lipids and lipoproteins, functions and immune suppression, cell division and DNA replication, membrane lipids, antioxidants, molecular-genetic analysis of RNA viruses, blood coagulation.

主要致力于生物技术的教学与研究，研究领域包括分子生物药物化学，亚病毒RNA病原体，脂类和脂蛋白的物质代谢，机能和免疫抑制，细胞分裂和DNA复制，膜脂，抗氧化剂，RNA病毒的分子－遗传分析，血凝固等。

Both immunohistochemical stainings and real-time mRNA techniques showed that this apoptosis-inducing effect was seen in HSC.

免疫组织化学染色和实时mRNA技术均显示出胶霉毒素对肝星状细胞的诱导凋亡作用。

Held jointly by the Institute of Biochemistry and Cell Biology, SIBS, CAS, National Institute of Cancer and Ben May Institute for Cancer Research of Chicago University, the symposium is a great success.

本次会议由上海生物化学与细胞生物学研究所、美国国家癌症研究所和美国芝加哥大学Ben May癌症研究所共同举办。

XU Ming-min GUO Li-he Institute of Biochemistry and Cell Biology ; SIBS ; CAS ; Shanghai

中国科学院上海生命科学研究院生物化学与细胞生物学研究所

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。