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细胞化学

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Methods The neural model of nerve growth factor differentiated PC12 cells and hypoxic model induced by cobalt chloride were made.

建立神经生长因子诱导PC12细胞分化和氯化钴诱导化学缺氧的神经元缺氧模型。

Caffeine is produced in coffee plant cells from a natural plant chemical in three steps.

咖啡因是由一种天然植物化学成分在植物细胞里分三个阶段制造出来的。

Result:Characteristics of the tangentical longitudinal section of stem with resin and surface view of leaves were elucidated.Besides xylem vessels and fibers of the stem,it was found that the red resin also exists in the cortex parenchyma cells of the stem and the medulla and xylem of the root.According to the HPLC fingerprint analysis result of the stems with and without resin,a number of flavones and stilbenoids were detected in the stem in which resin appeared after it wounded.

结果:阐明了含树脂茎的纵切结构以及叶的表面观特征;除了茎木质部导管和纤维中有树脂外,发现剑叶龙血树受到损伤之后在茎皮层薄壁细胞、根的髓部和木质部亦有树脂的分布;含树脂茎和无树脂茎的指纹图谱显示剑叶龙血树茎在形成树脂后的化学成分中出现了较多的黄酮类、茋类成分。

Compare the effect of different chemical methods for preparation of acellular nerve scaffold and to provide an effective nerve scaffold for tissue engineering.

目的 比较不同化学方法制备去细胞神经支架的效果,提供有效的组织工程神经支架。

To master the isolation of enzyme in cell .the concept ,mechanism ,the significance of Allosteric regulation of key enzyme .

掌握:细胞内酶的隔离分布;关键酶的变构调节—概念、机制、生理意义;酶的化学修饰调节—概念、特点。

Epilepsy rat models were made by injecting KA into amygdale under stereotactic instrument.The ERK1/2 protein expression in various groups were tested with immunohistological method.

采用立体定位仪下杏仁核内注射KA方法制备大鼠癫痫模型,于致痫后不同时程进行灌流固定、心肌组织的石蜡包埋、切片及免疫组织化学染色,检测不同时程心肌细胞ERK1/2表达的灰度值。

The investigation of laticifer differentiation has an important significance for developing chemistry-regulating technique in natural rubber production and for realizing the cambia differentiation mechanism.

乳管分化的研究对于发展天然橡胶生产的化学调控技术和认识维管形成层细胞的分化机制具有重要意义。

The chemical formulas differ in each system, and the technique of living cell reproduction is slightly different in each local universe, but the Life Carriers are always the living catalyzers who initiate the primordial reactions of material life; they are the instigators of the energy circuits of living matter.

在每个系统里化学公式不同,而生命细胞复制技术在每个本地宇宙里有少许不同,但生命携带者一直是天然的催化剂。他们发起生命物质原始反应;他们是活性物质能量电路的煽动者。

Methods Establishing a model of whole cerebral ischemia-reperfusion in rats; examining the expressions of TrkA and NGF on cochleas assuming the method of immunohistochemistry stain; and examining the time and distribution of the cochlear hair cell apoptosis through the terminal deoxynucleotidyl transferasemediated dUTP nick end labeling.

建立大鼠全脑缺血再灌注模型,采用免疫组织化学染色方法检测TrkA和NGF在耳蜗中的表达;用TUNEL法检测耳蜗毛细胞发生凋亡的时相和分布。

Results:The positive expression of P53 and c-Myc protein in nuclei was bound up with poor-differentiated tumours;the positive expression of Fas and c-Myc protein in cytoplasms was consistent with well-differentiated tumours.

采用免疫组织化学方法对骨肉瘤、骨化性纤维瘤、骨纤维结构不良和正常骨痂组织进行c-Myc、Fas和P53蛋白测定,与肿瘤组织学分级、细胞分化及核丝分裂比较分析。

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