细胞化学

In conclusion, we detailedly investigated the variation of membrane system (mainly plasmalemma, amyloplast envelope, ER) with the potassium permanganate fixative method, nucleus and organelles with routinely fixative method, and their functions with cytochemical method (localization of phosphatases and Ca〓).

综上所述，本文运用高锰酸钾固定法研究了膜系统（主要指质膜、淀粉质体被膜、内质网）的变化，运用常规固定制样研究了细胞核和细胞器的变化，采用细胞化学方法（Ca〓和磷酸水解酶的定位）探索了细胞器和细胞核的功能。

In vitro experiments, the expression of cytochrome C in glial cells was detected by western blot and immunofluorescence cytochemistry.

体外实验中，免疫蛋白印迹法和免疫荧光细胞化学法测定大鼠神经胶质细胞细胞色素C的表达。

NF-κB expression was detected by immunofluorescence cytochemistry, and the viability of the PC12 cells was demonstrated by MTT assay.

免疫荧光细胞化学法测定软骨藻酸对PC12细胞NF-κB蛋白的影响；MTT法检测PC12细胞的存活率。

OVCs were isolated by density ladder centrifugation in the 4th week, and then OVC's morphology was observed under transmission electromicroscrope and immunocytochemistry were performed to detect the expression of ICAM-1. Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast.

结果 透射电镜下观察到OVCs超微结构的改变为核大，核/浆比大，核仁小，胞浆内细胞器少，可见少量内质网和小线粒体，整个细胞呈幼稚、未分化状态；ICAM-1免疫细胞化学检测阳性；损伤肝组织HE染色和ICAM-1免疫组化检测显示，肝损伤初期，Hering管周围OVCs增生并且ICAM-1阳性表达，随着肝损伤的加重，OVCs继续增生，并且，OVCs沿增生的肝纤维向肝小叶迁移，同时，ICAM-1阳性表达也继续增多，并逐渐向肝小叶弥散分布；透射电镜下观察到，肝损伤初期，OVCs在Hering管周围增生，肝纤维随之增生，增生的OVCs与肝纤维黏附生长。

The plasticity of NSCs was detected by the differentiating test. HESCs were induced into dopaminergic neurons by means of adding Sonic Hedgehog Peptide and fibroblast growth factor eight (FGF8) early under the condition of mocked microenvironment and different development stage of dopaminergic neurons in vivo ,meanwhile made comparison with traditional method . The marker of dopaminergic neurons was tested by immunocytochemistry and RT-PCR, the content of dopamine and its derivation homovanillic acid were measured by HPLC-ECD.

模拟体内多巴胺神经元分化发育的不同阶段及微环境，采用早期添加音猥因子及成纤维细胞生长因子-8(FGF8)的方法，诱导hESCs定向生成多巴胺能神经元，并与传统的诱导方法相比较，免疫荧光细胞化学和RT-PCR法检测多巴胺能神经元的标志，高效液相色谱法检测诱导后细胞及细胞培养上清液中多巴胺及其代谢产物高香草酸的含量。

METHODS: 8-Bromo-cAMP and sodium nitroprusside were added into the media to raise the concentrations of cAMP and cGMP of cultured cardiomyocytes, in situ hybridization and immunohistochemistry methods were used to identify the mRNA and protein expression of bFGF. The amount of bFGF mRNA and protein expression were detected by computor imaging analysis system MIAS exe.

分离培养大鼠心肌细胞，加入8-溴-cAMP和硝普钠，升高细胞内cAMP、cGMP浓度，用原位杂交及免疫细胞化学的方法观察细胞内bFGF的表达，图象分析检测bFGF表达量的变化。

He experiment results indicate that (1) The extract of Rubia Cordifolia L has the effects of anti-proliferation and inducing APO;(2) Whenadministrating with 5-fu, the extract of Rubia Cordifolia L obviously enhanced the effects of anti-proliferation and inducing APO of 5-fu;(3) The basement of molecular biology of the extract of Rubia Cordifolia L for inducing APO of MGC-803 cells is correlated with inhibiting the expression of bcl-2 of anti-apoptosis gene.

UNEL法与PCNA的免疫细胞化学法的结果：0.2、0.4、0.8、1.0mg/ml浓度的茜草提取物与2、4、8、16μg/ml浓度的5-Fu，分别诱导细胞凋亡和抑制细胞增殖，表现出浓度、时间依赖性关系，除个别浓度与个别时间之间无显著性差异（P＞0.05）外，不同浓度与不同作用时间之间均有显著性差异（P＜0.05或P＜0.01），并且两种药物联合使用时，MGC-803细胞的APO%显著提高，降低PCNA的阳性表达率（P＜0.05或P＜0.01）。

Based on establishment of the human macrophage-derived foam cell model, the expression of Kv1.3 channel was investigated by immunocytochemical staining, reverse transcription-polymerase chain raction and Western blot. Furthermore, the effects of rMargatoxin, a Kv 1.3 channel-specific inhibitor, on cholesterol metabolism in macrophages incepting oxidized low density lipoprotein were studied.

在建立人巨噬细胞源性泡沫细胞模型的基础上，采用免疫细胞化学染色法、逆转录聚合酶链反应(transcription-polymerase chain raction，RT-PCR)及Western blot，研究Kv1.3通道的表达，并观察其特异性阻断剂--rMargatoxin对摄取氧化修饰低密度脂蛋白的巨噬细胞胆固醇代谢的影响。

MSC induced by 5-aza could be identified by the positive staining for Troponin I and Connexin43. The number of cells stained positively in the 10umol L-1 and 20umol L-1 5-aza was visually greater than that of other groups , but more than 50% the cells necrosed in 20umolL -1 5-aza. Electron microscopy revealed sarcomeric organization and gap junction.

结果MSCs体外贴壁生长，呈成纤维细胞样形态，经5一aza诱导后细胞体积增大，变为球状或者长杆状，4w后荧光免疫细胞化学方法检测肌钙蛋白、肌动蛋白及Connexin43表达阳性，10umol·L一'5一aza组及20umoz·L一'5一aza组阳性表达较高，但20umol·L一'5一aza组约50%细胞死亡。

Methods Based on the model of human umbilical vein endothelial cell,this study adopted Rose Bengal Stain,cell ELISA,immunocytochemistry techniques to investigate the effect of APS on lymphocyte-endothelium adhesion and the molecular mechanism.

以体外培养的人脐静脉内皮细胞为模型，应用蛋白染料染色、细胞ELISA、免疫细胞化学等方法，研究黄芪多糖对淋巴细胞与血管内皮细胞粘附的影响及其分子机制。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。