细胞化学

The journal publishes research reports and review articles that examine the molecular, cellular, and/or biochemical basis of toxicologically relevant outcomes.

该刊由Elsevier出版公司出版。本杂志主要发表与毒理学相关结果的分子的、细胞的及生物化学方面研究方面的研究论文和综述。

Then glutamate, GABA and GABA_BR1 in cells on bone trabecula surface of distal fermur of rats in every group were checked with immunohistochemistry method.

运用免疫组织化学方法检测各组大鼠远端股骨骨小梁表面细胞的Glu、GABA和GABA_BR1。

Ras homolog enriched in brain, a small GTPase, positively regulates the mTORC1 pathway. The GDP-GTP exchange of Rheb has been suggested to be facilitated by translationally controlled tumor protein. Here we demonstrate that human TCTP interacts with human Rheb and accelerates its GDP release in vitro, and that hTCTP activates the mTORC1 pathway in vivo.

丁建平研究组运用体外 GST-pulldown 和鸟嘌呤交换等生物化学实验以及对 TCTP 降低表达和过量表达等细胞生物学实验，证明了人源 TCTP 具有针对 Rheb 的弱 GEF 活性，能正向调控 mTOR 信号通路。

Tricarboxylic acid cycle (or Krebs cycle or citric-acid cycle): Last stage of the chemical processes by which living cells obtain energy from foodstuffs .

三羧酸循环(亦称克雷布斯氏循环或枸橼酸循环)：活细胞氧化食物并从中获得能量的化学过程的最终阶段。

We used the immunohistochemical ABC method to detect the expression of NF-κB at 2、 6、 12、 24、 48、 72 hours after infected by Salmonella typhimurium, and we deal the data statistically with univariant analysis of variance and Student-Newman-Keuls multiplerange test.

三组分别在感染鼠伤寒沙门氏菌后2、6、12、24、48、72小时6个时点，用免疫组织化学方法(抗NF-κB p65抗体、ABC法，石蜡包埋)和阳性细胞面积分布率半定量测定小肠、结肠及肾脏中NF-κB活性表达百分率，采用单变量多因素方差分析和Q检验做统计学处理。

ABSTRACT Objective To discuss the clinical pathologic features and differential diagnosis of mixed germ cell sex cord stromal tumorwith malignant variant of germ cell tumor Methods The clinical pathologic datum and immunophenotype were studied in the case of ovary MGCSCST with malignant variance of germ cell tumor Results The patient's genital anatomy and female phenotype were normal,karotypes was 46xx with uterogestation The tumor tissue consisted of mixed germ cells and sex cord stromal cells with evident malignancy of mixed germ cells There was no atypical structure of gonadoblastoma in tumor tissue Conclusion MGCSCSST has complex morphosis,thus,to prevent misdiagnosis,more samples should be collected and more slices be cut The patient may have prognosis malo when there are high malignant variants of mixed germ cells in tumor tissue

目的 探讨混合性生殖细胞性索间质肿瘤(mixed germ cell sex cord stromal tumor，MGCSCST)伴恶性生殖细胞肿瘤变异型的临床病理特点及鉴别诊断。方法卵巢MGCSCST伴恶性生殖细胞肿瘤变异型的病例进行临床病理特征和免疫表型分析。结果患者生殖器的解剖结构和女性表型正常，染色体组型是46xx，足月妊娠。肿瘤组织由混合性生殖细胞和性索间质肿瘤构成，混合性生殖细胞有显著的恶性特征。瘤组织中没有典型的性腺母细胞瘤结构。结论 MGCSCST有复杂的形态结构，因此更多的取材和切片可以防止误诊，当肿瘤组织中的混合性生殖细胞有高度恶性变异时，患者预后不良。卵巢肿瘤；生殖细胞性索间质肿瘤；免疫组织化学

The quantity and appearance of Foslike immunopositive neurons in ventrolateral part of PAG were investigated by immunocytochemical staining method.

采用免疫组织化学ABC法对大鼠中脑冰冻切片进行染色，显微镜下进行观察并拍照，对Fos免疫阳性细胞进行形态与计数分析。

Results as follows;(1) At 25th week of fetal age, the staining intensity and cell density almost equalled to that at birth in cervical spinal cord, brainstem, hippocampus, and vermis of cerebellum; while there are only 1/4 cell densities in cerebral cortex.

用胶质原纤维酸性蛋白抗体进行免疫组织化学染色。结果表明：(1)在颈段脊髓、脑干、海马和小脑蚓部于胚胎25周其GFAP染色强度、细胞密度接近出生时水平。

Key words: Schwann cell; Wallerian degeneration; artificial nerve; tissue engineering

10 d龄SD大鼠坐骨神经进行结扎预变性，分别于术后24 h、3 d、5 d、7 d和9 d，进行雪旺细胞培养和S-100免疫组织化学染色观察。

In this study, the DNA fragments coding for amino acids 133～158 of VP1and 20～34 of VP4protein of type Asial FMDV were chemically synthesized and ligated into a tandem repeat 133～158-20～34-133～158. The sequences of signal peptide of Igκ chain and Kozak were fused to the 5'end of this tandem sequence and synthetic oligodeoxynucleotide containing CpG-ISS was fused to downstream of terminal coden of this tandem sequence. And then this long fragment was cloned into the eukaryotic expression plasmid pHook-2, forming a new secreted expression plasmid, named pAS1-E.

在第二章证明了亚洲Ⅰ型口蹄疫病毒VP1蛋白中133～158位氨基酸残基确是一重要B细胞中和抗原表位的基础上，依据第一章获得的口蹄疫病毒亚洲Ⅰ型VP1 cDNA序列及已报道Asial VP4序列，采用真核偏爱密码子化学合成了VP1中编码133～158位氨基酸及VP4中编码20～34位氨基酸这两个抗原表位基因，将其组成133～158-20～34-133～158串联结构，在其5′端加上鼠Igκ链信号肽序列，在翻译调节区加上增强表达的Kozak序列，同时在133～158-20～34-133～158串联结构3'端终止密码子下游加上CpG免疫刺激序列，将这些片段连接后，克隆到真核表达载体pHook-2上，构建成功了DNA疫苗重组表达载体pAS1-E。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新