细胞化学

"Chemopreventive properties of Hibiscus sabdariffa L. on human gastric carcinoma cells through apoptosis induction and JNK/p38 MAPK signaling activation.

68，&生科所&，&陈璟贤&，，&助理教授&，&研究论文&，洛神花萃取物经由活化JNK/p38MAPK讯息路径诱导人类胃癌细胞凋谢死亡以达到化学预防之功效。

Immunohistolochemical stain by monoclonal antibodies for growth factors and actin was used to detect whether there are abnormal expressions in saccular aneurysms.

利用血小板源性生长因子、转化生长因子（TGF-β1）、血管内皮细胞生长因子和肌动蛋白的单克隆抗体进行免疫组织化学染色。

Methods:(1) Silica column chromatography and LH-20 were used in phytochemistry study.(2) Based on the extraction ratio of arctiin, the best extraction procedure was acheived under the direction of L9(34) orthogonal experimental design. From 10 types of resins, the one with the maximal binding ability for Arctii was selected and silica-column choramatograpy was used in its seperation and purification procedure.(3) The animal diabetic experimental model in Wistar rats was established with streptozotocin and many biochemical factors were used to indicate the pharmacological effects of arctiin, including glycosylated hemoglobin, serum creatinine, seralbumin, monoxide nitrogen, glycerin trilaurate, cholesterol, HDL-ch, LDL-ch, Blood urea nitrogen, creatinine, SOD, ET, MDA, total Urine protein and albuminuria.

(1)采用硅胶柱层析、聚酰胺层析、LH-20等方法对牛蒡子的化学成分进行研究；(2)采用正交实验设计方法，以牛蒡子苷为指标，确定了牛蒡子乙醇回流提取的最佳工艺；对10种类型大孔吸附树脂进行筛选，筛选出对牛蒡子苷吸附效果最佳的树脂；采用硅胶柱层析法，对牛蒡子苷进一步分离纯化；(3)建立实验性糖尿病大鼠模型，以GLU、TC、TG、HDL-ch、LDL-ch、TB、ALB、BUN、CREA、GHbAlc、NO、SOD、ET、MDA和尿液中TB、ALB为指标，研究牛蒡子苷药理活性；(4)以差速消化法纯化Wistar大鼠的主动脉内皮细胞并进行传代培养，测定牛蒡子苷对高糖条件下RAECs活性、RAECs释放LDH、MDA和NO的变化以及RAECs表达内皮型一氧化氮合酶表达的影响。

The inducible nitric oxide synthase is obviously expressed in speen of rats under the predator stress, suggesting important function for iNOS in speen physiology. However, the expression and subcellular localization for NOS in speen development and differentiation are largely unknown. In the present study, the changes in iNOS activity in spleen of rats under predator stress were studied to explore the role of iNOS in the decreased immunoactivity of the stressed rats. The changes in iNOS were observed with immunobistochemical method, and the contents of NOS and NO in spleen with biochemical method.

在捕食压力下，大鼠脾脏诱导型一氧化氮合酶明显表达，说明iNOS起到了重要的生理功能，然而，NOS的表达及在亚细胞水平的定位以及相应的差异变化等都很不明确，本试验采用捕食应激模型，利用免疫组织化学方法和生化测定，检测心理性应激对大鼠脾脏iNOS活性的影响及探讨这种影响与应激动物免疫力下降之间的关系。

Organic matrixs coming from cells play role in nucleation, growth, morphology, polymorphology of inorganic materials. This control of organic matrixs are mainly behaved by the interfaction between organic matrix and inorganic materials .The processing of organic matrix controlling inorganic material is called as molecular recognition. The mechanism of organic-inorganic molecular recognition contains three facts such as electrostatic attraction, lattice geometry matching and stereochemical complimentary.

细胞分泌的有机基质在无机矿物的成核，生长，形貌，多型和结晶学定向等过程中起到了调控作用，基质对矿化的这种调控作用具体表现在有机或生物大分子和无机离子在界面上的相互作用，有机质对无机晶体的成核，生长，晶形及结晶学定向等的控制过程称为分子识别，有机-无机界面分子识别机制主要包括静电作用，晶格几何匹配和立体化学互补三个方面。

Then rAAV-DJ-1 was transfected into HEK-293 cells for packaging. AAV vectors encoding human DJ-1 protein were stereotactically injected into the rat SN 4 weeks prior to rotenone lesion in SN. Tyrosine hydroxylase protein was determined using immunohistochemical staining. Ethovison software allowed measurement of movement distance in 30 minutes.

并转染HEK-293细胞，进行腺相关假病毒颗粒包装，所得病毒颗粒注射到大鼠脑内感染黑质神经细胞；4周后，注射鱼藤酮于大鼠黑质相同脑区；通过免疫组织化学方法鉴定TH蛋白表达情况，采用动物行为轨迹分析软件计算大鼠30 min内运动距离。

METHODS: The neonatal and adult rats facial nerve was ligated at stylomastoid foramen.

于新生及成年大鼠茎乳孔处结扎面神经，于3， 7， 14， 21， 28， 56， 84 d采用NADPHd组织化学方法观察NADPHd阳性细胞在面神经核中的变化。

The gray value of SP and CGRP radioactive particles in bilateral spinal trigeminal caudal subnucleus in rats were detected by immunohisto- chemisrty while obvious damages in trigeminal neurocytes on the injected side were confirmed.

在确认实验侧三叉神经节细胞出现明显破坏的同时，采用免疫组织化学技术，检测大鼠双侧三叉神经脊束核尾侧亚核内P物质和降钙素基因相关肽免疫阳性颗粒的灰度值。

Methods TGFβ1 protein in 53 cases of RA synovia ［including 26 cases of RA,20 cases of control non-RA (14 cases of OA and 6 cases of traumatic arthritis) and 7 cases of normal synovia］ was detected by immunohistochemical LSAB method.

采用免疫组织化学LSAB法检测了53例(其中26例RA，20例其他关节炎和7例正常人)滑膜组织中TGFβ1蛋白的表达情况，并用目镜网格测微尺和图像分析系统分别进行TGFβ1阳性细胞的半定量计数和TGFβ1染色强度及面积的定量分析。

Methods: 60 histologically verified HCC and 42 non-HCC specimens were examined for Topo-Ⅱ by immunohistochemical methods.

应用免疫组织化学S-P法检测60例原发性肝细胞瘤及42例非肝癌肝组织中Topo-Ⅱ的表达。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新