细胞化学

This experiment take one-day-old chickens as the object of study, we apply cell raise technologyand MTT method, the indirect enzyme-lined immunosorbent assay, the immunity enzymehistochemistry methods andα-naphthyl acetate esterase etc..

本实验以1日龄雏鸡为研究对象，应用细胞培养技术和MTT法、间接酶联免疫吸附实验、免疫酶组织化学和组织酸性α-醋酸萘酯酶染色法等方法。

Methods Immunohistochemistry stain was employed on the 34 lupus nephridial tissues and 10 nephridial tissues of control group. And light microscopy was applied to observe the percentage of CKLF-1 positive cells of renal gromerulus and tubule.

对34例确诊为狼疮性肾炎以及10例对照组患者的肾组织进行免疫组织化学染色，在光镜下观察肾小球CKLF-1阳性细胞率和CKLF-1阳性肾小管百分率及反应强度。

Using neuroanatomical tracing technique and immunohistochemical staining methods, we studied whether transplantation of adult bone marrow stromal cells could promote axon regeneration after spinal injury.

本篇文章利用神经解剖追踪及免疫化学染色的方法探讨脊髓损伤后移植骨髓基质细胞是否帮助脊髓再生。

Objective To investigate clinicopathological characteristics of unicystic ameloblastoma and histological markers for differential diagnosis with odontogenic cyst.

目的 研究单囊型成釉细胞瘤的临床病理及凝集素免疫组织化学特点，探索有助于诊断和鉴别诊断的组织学标记物。

The Research of the equine diseases and the Analysis of their reasons in Henan Province;2. Epidemiological investigation about equine tumors in Gansu province;3. The results showed that prepubertal orchidectomy of the equine resulted in a slight but not significant increase in the number and percentage of gonadotrophs,but significantly increased the average area of .

利用免疫组织化学和形态计测学的方法，观察了 7匹公马和 6匹在 1～ 2岁期间被摘除了睾丸的阉割马的脑垂体前叶促性腺激素细胞的数量和面积，同时利用放射免疫分析方法检测了血浆中垂体促卵泡激素和垂体促黄体激素的水平。

The aim of this study was to investigate the expression of mPGES-2 protein in mouse oviduct during the early pregnancy, pseudopregnancy, and estrous cycle by immunohistochemistry and Western blot. The regulation of mPGES-2 protein in mouse oviducts was also studied under delayed implantation, steroid hormonal treatments and oviductal ligation.

本实验主要利用早期妊娠、假孕、输卵管结扎、发情周期、激素调节以及延迟着床等实验模型，结合免疫组织化学和Western blot 的实验方法，详细研究了mPGES-2 蛋白在小鼠输卵管中的表达与调节。mPGES-2 蛋白主要定位于不同时期的伞部和壶腹部的上皮纤毛细胞中，在伞部和壶腹部肌层以及峡部和宫管连接处的上皮和肌层中未检测到mPGES-2 蛋白的表达。

Methods Pancreatic cancer cell panc-1 was treated with oxamate for 48 h,the mitochondrial membrane potential was measured by flow cytometry(Rodamin-123),and the activity of intracellular lactate dehydrogenase was studied by enzymohistochemistry.

方法体外培养人胰腺癌细胞panc-1，分别加入不同浓度的草氨酸盐培养48 h后，行酶组织化学染色及罗丹明123染色，观察细胞糖酵解关键酶乳酸脱氢酶活性及线粒体膜电位。

Methods: By reverse transcriptase polymerase chain reaction and immunohisto-chemical assay, the expression of Surviving gene and bcl-2 protein were detected in 16 cases normal tissue of bladder, 12 cases papillate tumor and 59 cases transitional cell cancer of bladder.

采用逆转录PCR方法检测了16例正常膀胱组织、12例膀胱移行细胞乳头状瘤和59例膀胱TCC组织Survivin基因的表达，同时用免疫组化学方法检测以上组织中bcl-2蛋白的表达，并将结果进行相关分析。

We used the avidin-biotin complex technique and EGFR monoclonal antibody to evaluate the expression of EGFR in 29 cases of cholesteatoma and 34 samples of normal postauricular skin. Of patients with cholesteatoma, 79%(23 cases) had EGFR-positive cells in the basal layer, 66%(19 cases) in the parabasal layer, and 62%(18 cases) in the upper layer of the epithelial tissue.

本研究利用免疫组织化学酵素抗体法（avidin-biotin-peroxidase complex，ABC method）以 EGFR 单株抗体免疫染色，评估29例胆脂瘤与34例耳廓后皮肤两者组织上皮内 EGFR 阳性细胞之分布表现。

Parthenogenetic activation is artificially induced by various kinds of physical and/or chemical methods.

人工孤雌激活方法包括物理性激活和化学性激活以及联合激活方法，人卵母细胞的孤雌激活多以化学激活为主。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新