细胞化学

By using the immunohistochemistry method of Strept Avidin-Biotin-Complex, four kinds of antisera raised against rabbit hormones: somatostatin, pancreatic polypeptide, 5-hydroxytryptamine （ 5-HT ） and neuropeptide Y were used to indentify and locate the endocrine cells in the digestive tract of Sparus latus.

应用4种兔抗胃肠激素抗体和SABC免疫组织化学方法，对黄鳍鲷消化道中的内分泌细胞进行鉴别和定位。

Was simultaneously injected into cisterna magna at the second injection blood in the presence of subarachnoid blood. At 2 to 4 days, GFP was expressed in leptomeninges over the brain stem, cortex and cerebral arteries, smooth muscle cells of small vessels were occasionlly transduced. GFP was expressed in adventitia of spastic basilar artery on days 2 (day 5 after first injection blood) after injection 〓, but was undetectable by days 4, transgene was not expressed in medial or intimal layers. The prensence of subarachnoid blood can not prevent access of adenovirus to vessels and transgene expression.

注入腺病毒载体后2天（初次注血第5天）行荧光显微镜、免疫组织化学和RT-PCR检测，结果在颅内大血管如基底动脉的外膜可见外源基因的表达，而外源基因不能转移至血管中膜和内膜，4天时（初次注血第7天）基底动脉的外膜中外源基因表达消失；注入腺病毒载体后2～4天，外源基因可以有效转移至颅底软脑膜细胞，小血管外膜和平滑肌层也可见外源基因的表达，表明蛛网膜下腔内的血凝块不能阻止腺病毒载体介导外源基因转移至颅内血管。

The Immunohistochemical results indicated that the pI〓 is obviously located on the liminal surface membrane of the distal tubules and Henle's ascending limbs, and can also be found at Henle's loop of porcine kidneys, these segments expose in non-isotonic condition.

免疫组织化学染色显示，pI〓在猪肾脏明显定位于非等渗区域的远曲小管和髓袢升支粗段的管腔表面膜及髓袢处，而等渗区域的近曲小管细胞几乎无pI〓的存在，据此认为pI〓可能在耐受渗透压的变化上发挥作用。

Methods: Using chemiluminescence assay to determine phagocytic function of celiac macrophagocyte and the erythrocyte immunization of the mouse model by reserpine.

采用化学发光测定和分析的方法，对利血平造模的小鼠进行腹腔巨噬细胞吞噬功能和红细胞免疫功能的测定。

Methods Both in malformed embryos induced by excess retinoid acid and normal KM mice, expressions of lectin receptors of WGA, RCA and ECL in the epithelium of palatal shelves and the mesenchyma were determined by affinity histochemistry using biotin-marked corresponding lectins.

以生物素标记的凝集素受体WGA、RCA和ECL为探针，应用亲和细胞组织化学染色方法检测上述3种凝集素受体在RA致畸鼠胚和相应对照组鼠胚腭板上皮及其间充质的分布状况和变化规律。

The central part was composed of hypertrophic chondrocytes, for which the number increased with time. The cartilaginous tissue at the time of 2 weeks' cultivation appeared strongly positive staining of collagen type Ⅱ immunohistochemistry and safranine "O", as well as strongly metachromatic to toluidine blue.

其中心为肥大软骨细胞，且随培养时间的延长而增多。2周时，软骨组织甲苯胺蓝染色呈强的红色异染反应，Ⅱ型胶原免疫组织化学染色呈强阳性，番红&O&染色呈强阳性。

The cellular structure, chemical characterization of foliar endodermis and element cellular distribution were investigated in Pinus bungeana leaves by the fluorescence microscope, Fourier transform infrared microspectroscopy and energy-dispersive X-ray analysis.

应用荧光显微技术、傅里叶变换显微红外光谱分析、扫描电镜及X-射线能谱微区分析等手段，对白皮松子叶、初生叶及2 a生针叶内皮层细胞径向壁的显微结构特征、化学成分，以及在叶子横切面上Na和Cl的微区分布进行分析。

To observe the influence of tumor necrosis factora on differentiation of rat mesencephalic neural stem cells,the numbers of neurons,astrocytes and oligodendrocytes generated from NSCs were analyzed after differentiation for 3 days by using i m munocytoche mistry technique.

为观察肿瘤坏死因子对神经干细胞分化的影响，本研究应用体外扩增的新生大鼠中脑NSCs，使用免疫组织化学技术，观察了肿瘤坏死因子一。对NSC、分化及其后代细胞的影响。

In this paper, we review recent advances on mucilaginous seeds, with emphasis on （1） chemical composition of seed mucilage;（2） morphological characteristics of mucilaginous seed coats and mucilage;（3） biochemical processes in and genetic regulation of differentiation of mucilage-secreting cells, mucilage biosynthesis and mucilage secretion in the seed coat; and （4） ecological significance of seed mucilage.

为此，在广泛查阅相关文献的基础上，该文综合分析了国内外种子粘液的研究进展，并重点探讨了以下几方面问题：（1）种子粘液的化学成分：（2）粘液及粘液种皮的形态特征：（3）粘液细胞分化与粘液生物合成的细胞学及基因调控机制以及粘液的释放方式：（4）种子粘液的生态学意义。

Methods 124 DFSPs were reviewed by light microscopy. 6 cases with areas of myoid/myofibroblastic differentiation were assayed with immunohisto-chemical technique and electron microscopy was applied in two cases.

采用常规HE切片对124例DFSP进行筛选，对6例伴有肌样／肌纤维母细胞性分化的DFSP病例进行免疫组织化学标记，其中2例加做电镜检测。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新