细胞化学

Methods The constituents in the 60% ethanol extract of Drynaria fortunei were isolated by a combination of silica gel, Sephadex LH-20 and ODS chromatographies and their structures were identified by spectral methods.

采用硅胶、Sephadex LH-20、ODS柱色谱分离骨碎补根茎的活性成分，应用理化方法及1D-NMR、2D-NMR方法确定化学结构，并检测对UMR 106细胞增殖的影响。

In double labell the high contrast substrates of enzym should be used.

因而作者认为，在进行胰岛内分泌细胞的双重免疫组化染色时，一染后可经PBS漂洗后直接进行第二种抗原的免疫组化染色，既节省时间，又减少了因不同的化学试剂的应用而影响第二种抗原活性的可能性。

The cells from hippocampus, stria-tum, ependyma, thalamus, olfactory bulb, cortex, brain stem and cerebellum of the brain were studied using HE staining and im-munocytochemistry.

应用苏木精-伊红染色和免疫组织化学方法观察人胎脑室管膜下区、海马、纹状体、丘脑、嗅球、皮质、脑干及小脑的Nestin阳性细胞。

In the present study, the ependymal tanycytes of the third ventricle in preoptic region of rat were investigated using NADPH-diaphorasehistochemistry.

应用NADPH-d组织化学方法研究了大鼠第三脑室视前区室管膜的伸展细胞。

Gimelbrant et al. determined the proportion of human genes that can be expressed monoallelically, in patterns that are epigenetically stable (for example, chemical modifications of DNA, such as cytosine methylation, that do not alter the sequence but are heritable within cell populations).

Gimelbrant等确定部分人类基因，能够单等位基因性表达，在表达模式上，后生同类物稳定（例如，化学修饰的DNA ，如胞嘧啶甲基化，不改变序列，但内细胞群是可遗传的）。

It will provide the rational for exploring the ideal treatment for liver cancer by detecting the expression of c-Met,hTERT and MAGE-1,which has high expression in liver cancer,in cancer tissues and tissues surrounding tumor by immunohistochemistry,analyzing the different expression of gene in tumor tissue and tissues with different distance from tumor,and then initially debate the molecular boundary in liver tumor exairesis.

通过免疫组织化学方法检测原发性肝细胞癌组织中高表达基因c-Met、hTERT、MAGE-1的表达以及它们在肝癌癌周组织中的表达，分析基因在肝癌组织和癌周不同距离组织的差异表达，初步探讨肝癌的分子切缘，为探索新的治疗肝癌途径奠定坚实的理论基础。

Methods Nuclear β-catenin expression was detected by immunohistochemistry in 77 lesions with desmoid-type fibromatosis and 171 other spindle cell lesions, including superficial fibrmoatosis (n=18), nodular fasciitis (n=36), keloid (n=16), scar (n=10), granulation tissue (n=9), synovial sarcoma (n=38), neufibroma (n=13), solitary fibrous tumor (n=12), gastrointestinal stromal tumor (n=10), low-grade myxofibrosarcoma (n=3), low-grade fibromyxoid sarcoma (n=3), and smooth muscle tumor (n=10). In addition, the immunohistochemical expressions of ER-α, ER-β and Ki-67 were examined in all of the lesions with desmoid-type fibromatosis. The nuclear immunohistochemical staining for nuclear βcatenin and ER-β was graded as high level (≥25% of cells), low level (5%-25%) or none.

采用免疫组织化学染色法检测77例韧带样型纤维瘤病和171例其它良、恶性梭形细胞病变（包括结节性筋膜炎36例、浅部纤维瘤病11例、瘢痕疙瘩16例、增生性瘢痕10例、肉芽组织9例、滑膜肉瘤38例、神经纤维瘤13例、孤立性纤维性肿瘤12例、胃肠间质肿瘤10例、低度恶性黏液纤维肉瘤3例、低度恶性纤维黏液样肉瘤3例及平滑肌肿瘤10例）组织中β-catenin核阳性的表达，同时检测韧带样型纤维瘤病中ER-α、ER-β和Ki-67的表达。

Immunohistochemistry assays were applied to detect the protein expression of MMP-9 and PAI-1 in arteriovenous fistular vascular segments.

通过免疫组织化学技术，在蛋白质水平检测36例动静脉内瘘血管内皮细胞中的MMP-9和 PAI-1的变化。

In this research, the pubertal five-week-old SD rats were chose as experimental object to establish the animal model for simulating maxillary protraction, on which was imposed the orthopedic force of 85g with utilizing the self-devised maxillary protraction appliance. Located lateral X-ray cephalograms were taken to measure and analyze the changes of maxillary development after exerting the orthopedic force for 4 weeks. The technique of immunohistochemistry was used to investigate theexpression of TGF-, in frontomaxillary sutures and palatomaxillary sutures with identical force performing for different extent of time. The average hue was selected as indicator, which was measured by the means of the analysis system of pathological color images. The measuring data were analyzed by the one-way ANOVA in software SPSS 11.0 version.

本研究采用自行设计的上颌前牵引装置，以生长发育期5周龄SD大鼠为实验对象，施加85g的矫形力，建立了模拟上颌前牵引的动物模型，通过X线头影测量分析，观察施力4周后上颌骨的生长发育情况；应用免疫组织化学技术，检测同一力值作用不同时段下TGF-β_1在上颌骨的额颌缝、腭颌缝的表达，彩色病理图象分析系统测定每张切片中阳性染色细胞的平均灰度值，结果采用SPSS 11.0进行方差分析。

This is because neuroanl damage, reactive gliosis or loss of GFAP has not yet completed in such a short duration after an ischemic stroke.

因为在数小时内神经细胞死亡及胶质细胞增生之组织型态学以及GFAP 染色丧失或反应性增加之组织化学的变化皆尚未完成。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新