细胞化学

Subcellular localization of ATPase activity in the flesh of developing apple fruit was carried outusing a lead phosphate precipitation technique via electron-microscope. The ultrastrcture of phloemand its surrounding cells was also observed. The results showed that ATPase activity wasdominantly localized on the plasma membranes of sieve element/companion cell complex.

应用铅沉淀的细胞化学方法，研究了发育过程中苹果果肉内卸载区(韧皮部及其周围薄壁细胞)ATP酶的亚细胞分布和变化，并且，作为补充，观察了韧皮部及其周围薄壁细胞的超微结构。

Contents include cell culture 、 cell fusion 、 Cytochemistry 、 apoptosis detection 、 protein expression and function determination , etc.

课程涉及的主要实验内容包括细胞培养、细胞融合、细胞化学、细胞凋亡检测和蛋白质的表达与功能检测等。

As the development of monoclonal antibody and quantitated fluorescent cytochemistry technique, FCM has been used more extensively in biology, clinical medicine, pharmacology, material and so on.

本文就流式细胞术的发展历史、流式细胞仪的原理及在各领域的应用进行综述，阐明现代流式细胞术由于结合单克隆抗体技术、定量荧光细胞化学技术，使其在生物学、临床医学、药物学、材料学等众多研究领域中的应用有更加突飞猛进的发展。

Methods Keratinocytes monolayers and Malassezia furfur were co-cultured in vitro, and the modulating effects of Malassezia furfur on the expression of TLR-2 was measured by immuocytochemistry methods, the expression and secretion of sICAM-1 and IL-8 in keratinocytes was detected by enzyme linked immunosorbent assay.

马拉色菌和角质形成细胞体外共培养，通过免疫细胞化学方法检测角质形成细胞TLR-2蛋白的表达状况，并运用ELISA方法检测马拉色菌对角质形成细胞sICAM-1和IL-8表达和分泌的调节作用。

Methods:Phenobarbital and Valproic acid were added into astrocytes and cultured permanently for 30 days. P-glycoprotein, a product of MDR1, was detected with immunocyto chemistry. Then, 5μg/ml Sibelium was added to resistance cells and nonresistance cells, MTT assay was used to measure reverse multiple and relative reverse efficiency rate.

将苯巴比妥钠和丙戊酸钠分别加入培养的星形胶质细胞内，30天后用免疫细胞化学法检测MDR1的表达产物P-糖蛋白，继之将终浓度为5μg/ml的西比灵分别加入培养的正常细胞及MDR1表达增强的细胞内，用四甲基偶氮唑盐法计算逆转倍数和相对逆转效率，了解西比灵逆转MDR1表达的作用。

The surface antigen expressions of MNC were detected by flow cytometry. MNC were induced with DMEM medium, containing HGF alone, FGF4 alone, HGF+FGF4 or no growth factor, respectively. The markers of hepatic linear cells were examined before induction, and 7, 14, 21 and 28 d after induction by RT-PCR, immunocytochemistry and periodic acid-schiff in every group.

分别用肝细胞生长因子、成纤维细胞生长因子-4(FGF4)、HGF+FGF4、无生长因子4种处理因素进行诱导培养，于诱导前及诱导后的第7、14、21、28天采用RT-PCR检测AFP、白蛋白及C-met、FGFR2 mRNA的表达，免疫细胞化学法检测肝细胞抗原和CK18的表达，PAS 法进行糖原染色。

The ultrastructures of the SP, VIP secretory cells showed there were several kinds of cells with different morphology which could express these neuropeptides in sacculus rotundus.

免疫电镜细胞化学观察发现在兔圆小囊内除了DNES等细胞内有SP和VIP的分布以外，在细胞间隙和淋巴细胞、巨噬细胞等的胞浆膜上也常见SP和VIP免疫电镜阳性反应信号。

In this study, after incubating cultured human umb ilical vein endothelial cells with TNF-α for different time, we employed RT-PCR to assay IL-8 mRNA expression and immunocytochemical stain ing to detect NF-κB activation in HUVECs.

本文用TNF-α孵育培养的人脐静脉内皮细胞不同时间后，用RT-PCR法检测内皮细胞内IL-8 mRNA的表达，并用免疫细胞化学染色法检测内皮细胞内NF-κB的激活。

MAIN OUTCOME MEASURES: Morphology changes of induced cells; introduced endothelioid cell surface antigen detected through immunocytochemical staining; the growth and adhesion condition of endothelioid cells on the intravascular stent observed under scanning electronic microsope.

主要观察指标：诱导后细胞形态变化，免疫细胞化学染色检测诱导后内皮样细胞表面相关抗原，扫描电镜观察内皮样细胞在血管支架上的生长黏附情况。

The distribution of the growth-associated protein (GAP-43) which is close related to the axon regeneration was examined by immunocytochemical method in the adult rat brain and the rat parietal lobus injuried into which the fetal cerebral suspension was lransplanted.

在发现流式细胞计可用于神经科学的研究后，用FCM研究了大鼠脑发育过程中细胞周期的变化和胚胎大脑细胞移植到成年大鼠顶叶后对宿主顶叶脑细胞分裂的影响；由于生长相关蛋白(GAP-43)与轴突再生密切相关，用免疫细胞化学法研究了GAP-43在成年大鼠脑内的分布及顶叶损伤植入胚胎大脑细胞后GAP-43的变化。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。