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细胞化学

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This fact led me to speculate that an axon could find its retinal cell neighbors in the tectum by homing in on chemical scents released by active tectal neurons, because its neighbors were most likely at the source of this trail .

这让我怀疑,轴突是否可以藉著活跃的顶盖神经元释出的化学物质,找到顶盖里的视网膜细胞邻居,因为它的邻居最有可能就在这条路的起点。

For the first time in the world, an in vitro testing model based on FRTL-5 cell line for thyroglobulin disruptors was established, as well as an in vivo testing model acting as a compensation for the in vitro method. The feasibility of the combination of in vitro and in vivo screening methods was discussed, as well as the mechanism of thyroid hormone disruptors.

为达到这一目的,我们在国内外率先建立了FRTL-5细胞对干扰甲状腺球蛋白的化学物的体外甄别方法模型,并建立相应的体内动物实验模型以弥补体外甄别方法在特异性上的不足,探讨以此建立体外、体内试验相结合的甄别方法体系的可行性,同时初步探讨甲状腺素干扰物的作用机制。

In this study, we make a systematic observation on localazation of mast cells in rabbit by toluidine blue staining method.

本研究采用甲苯胺蓝染色法和免疫组织化学方法,对肥大细胞(Mast cell,MC)在家兔体内的分布定位进行了系统观察。

Collagen Ⅱ immunohistochemistry and toluidine blue staining had a weak expression on primary and subcultured cells.

原代和传代后细胞Ⅱ型胶原免疫组织化学和甲苯胺蓝异染反应均较弱。

The phenotype, calcium tuberculation and the expression of alkaline phosphatase were studied with alizarin red staining and modified Gomori Ca-Co assays respectively.

采用碱性磷酸酶Gomori 钙钴法染色、钙结节茜素红法染色及Ⅰ型胶原免疫组织化学染色方法进行鉴定成骨细胞生物学特性。

A series of indexes reflecting free radical changes, anti-oxidations, Immune function, endocrine function was studied by Chemiluminescence method, immunoturbidimetry, ANAE dye method, micro-hemagglutination inhibition test and radio immunoassay in the chicken with encephalomalicia induced by giving the feed being deficient in VE and extra fish oil artificially. The pathological changes including microscopic and ultramicroscopic changes in the relative organs and tissues were observed.

本实验以一日龄150只海兰兰蛋鸡为实验动物,用低维生素E且添加鱼油日粮饲喂雏鸡,复制脑软化症动物模型,采用化学发光法、免疫浊度测定法、酸性α-醋酸萘脂酶染色法、微量血凝抑制试验、放射免疫测定技术等方法系统地检测了脑软化雏鸡的自由基代谢和抗氧化功能、红细胞免疫功能、细胞免疫功能、体液免疫功能及非特异性免疫功能等指标,并对患雏病理组织进行了显微及超微结构观察。

MAIN OUTCOME MEASURES: At 15 days after model preparation, one rat was obtained from each group to underdone morphological observation. At 21 and 30 days after model preparation, 8 rats were killed in each group, hematoxylin-eosin staining and immunohistochemical method were used to detect the expression of the proliferating cell nuclear antigen, and to calculate fibroblast proliferation index.

主要观察指标:于造模后15 d各组取1只大鼠行组织形态学观察;造模后21,30 d,每组各处死8只大鼠,取材后,行苏木精-伊红染色,免疫组织化学检测增殖细胞核抗原的表达,并计算成纤维细胞的增殖指数。

Intimal areas were measured using morphometric analysis of perfusion-fixed vein graft specimens, and intimal thickness was calculated using circumferential measurements. The SMC proliferation was studied by the immunohistochemical detection of proliferating cell nuclear antigen. Expression of MMP-2、MMP-9 mRNA in vein grafts and unoperated control Vein grafts was detected by reverse transcription polymerase chain reaction. Substrate gel zymography was used to determine the proteolytic activity.

分别行HE染色、Verhoeff弹性纤维染色观察组织病理变化,计算机病理图象分析系统测量新生内膜厚度及面积,免疫组织化学方法检测静脉壁增殖细胞核抗原表达以观察细胞增殖情况,半定量逆转录-聚合酶链反应检测静脉壁基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)mRNA的表达,明胶酶谱法检测MMP-2、MMP-9活性,比较各组之间的差别。

Methods 34 cases were invesigated under the light microscopic l e vel and were studied by immunohistochemical technics.

报告34例鼻腔圆形细胞恶性肿瘤,观察其病理组织学表现,用免疫组织化学方法标记肿瘤。

Veratrine has anticarcinogenic chemical activity in the key stages of the development of cancer cells. It can kill the cancer cells and has no harm to the healthy cells.

白藜芦醇在癌细胞的起始,促进,发展三个重要阶段均有化学防癌的活性,能杀死癌细胞而对正常的细胞无毒害作用。

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