细胞化学

Objective To explore the effect of controlled release of nerve growth factor on peripheral nerve defect repaire by acellular nerve graft.

目的 探讨化学去细胞异体神经复合缓释神经生长因子（nerve growth factor，NGF）修复周围神经缺损的效果。

Reticulocyte analyzes were carried out by flow cytometry and sTfR and s-Epo levels determined by immunonephelometry and chemiluminescence, respectively.

网织红细胞分析进行了流式细胞仪和stfr和S -欧洲专利局的水平取决于immunonephelometry和化学发光，分别。

In addition,the expression of Galβ-1,4-GlcNAc group was studied by ricinus communis agglutinin-I lectin blot and lectin histochemistry with RCA-I respectively.

并利用特异识别-β1，4-半乳糖苷键的蓖麻凝集素-Ⅰ，经letin blot和组织化学方法分析-β1，4-半乳糖苷键在各型人脑星形胶质细胞瘤中的表达变化和表达定位。

Methods Mouse models of tibia fracture healing were established, and callus samples were collected 1, 3, 7, 14, 21 and 28 days after fracture. The development of callus and new bone formation were evaluated with roentgenology, Micro-CT and tetracycline double labeling method, and the expression of HIF-1α, vascular endothelial growth factor, Runx2 and ALP in callus were detected with RT-PCR, Western blotting and immunohistochemistry. The relationship between HIF-1α and fracture healing was analysed.

建立小鼠胫骨骨折模型，骨折后1、3、7、14、21、28 d取材，应用X线摄片、Micro-CT和四环素荧光双标记技术，观测骨痂组织的演变和新骨形成状况；采用RT-PCR、Western blotting和免疫组织化学方法，检测骨痂组织细胞HIF-1α、血管内皮生长因子和成骨性标志物（Runx2和ALP）的表达状况，分析HIF-1α与骨折愈合进程的关系。

Methods :The Alcianblue Safranin and immunohistochemical staining were employed to observe the density of MC, PCNA and oncogene bcl-2 expression in the specimens of 74 cases with gastric cancer.

采用阿尔辛蓝-沙红及免疫组织化学染色法，同步检测74例胃癌标本中的肥大细胞数及PCNA、bcl-2基因蛋白的表达。

The central part was composed of hypertrophic chondrocytes, for which the number increased with time. The cartilaginous tissue at the time of 2 weeks' cultivation appeared strongly positive staining of collagen type Ⅱ immunohistochemistry and safranine "O", as well as strongly metachromatic to toluidine blue.

其中心为肥大软骨细胞，且随培养时间的延长而增多。2周时，软骨组织甲苯胺蓝染色呈强的红色异染反应，Ⅱ型胶原免疫组织化学染色呈强阳性，番红"O"染色呈强阳性。

RESULTS: HE and safranine O staining both showed no cellular structure in the matrix with only recesses left by the removed cells.

软骨蛋白聚糖免疫组织化学染色阳性，提示脱细胞关节软骨基质内仍存在软骨蛋白聚糖。

6β-santonin could be selectively reduced and hydroxylated by the cell suspension cultures of P.acinosa, which would provide valuable intermediates for its further structural modification.

结论利用商陆培养细胞可对6β-santonin进行选择性还原及羟基化反应，可为其进一步化学结构改造提供有价值的中间体。

METHODS: We performed an inmunohistochemical analysis of 204 SCLC samples to determine KIT protein expression.

本研究使用免疫组织化学方法分析了204例小细胞肺癌组织标本，用来测定KIT蛋白的表达情况。

Apoptotic index, the expression of Bax and Bcl-2 protein in myocardium were analyzed semiquantitatively by TUNEL and immunohistochemical assay 1,2,4,6,8,10,12 hours after coronary ligation.

2应用TUNEL法和免疫组织化学方法检测实验性大鼠AMI过程中心肌细胞凋亡及凋亡相关蛋白Bcl-2和Bax的表达情况。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新