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Because tumor response to cytotoxic chemotherapy has been poor, chemotherapy is used only for palliation.

因为肿瘤对细胞毒性化学治疗反应差,化疗仅用于减轻症状。

Becausetumor response to cytotoxic chemotherapy has been poor,chemotherapy is used only for palliation.

因为肿瘤对细胞毒性化学治疗反应差,化疗仅用于减轻症状。

The methods and data of phytomorphology. phytotomy, phytoembryology. palynology. phytocytota.xonomy. phytochemistry and molecular systematics are referred to summarize the systematic status of the genus Gnetum in this paper.

本文参考植物形态学、植物解剖学、植物胚胎学、孢粉学、植物细胞分类学、植物化学和分子系统学等方面的资料,对买麻藤属植物的系统学位置进行总结。

Methods Healthy male SD rats of different age groups were recruited(1-, 2- and 3- week groups, 1- and 2- month groups, 6- month grown group, and 18- month aged group; n=5 each). Immunohistochemistry was performed on the coronal sections of brain of the rats perfused with paraformaldehyde.

健康雄性SD大鼠,分为7天、2周、3周、1月、2月、成年(6月龄)和老年(18月龄)7组,每组5只,共35只,多聚甲醛灌注固定,冰冻冠状切片,免疫组织化学染色,光镜观察摄像,细胞计数。

All of the specimens collected were divided into two parts, one part for immunohistochemical staining after fixated by 4% paraformaldehyde, the other part for culturing fibroblasts.

所有取材部位未经任何治疗,标本经无菌取材后分成2份,其中一份用40 g/L多聚甲醛固定后用于免疫组织化学检测,另一份用于成纤维细胞培养。

Perceptively on 3d and 5d. Collagen is positive in Western blot on 9d. Aniline dyeing is positive on 12d. The control group was negative. Ad-BMP-2 transferred MSCs cultured in fibrin gel was positive in Aniline dyeing, Immunohistochemical. The therapy effect of group was better in histology, biochemistry and biomechanics in all periods than the other two groups, and was similar to the natural cartilage.

结果 ①Ad-BMP-2转基因MSC BMP-2、CollagenⅡ的PT-PCR分别在3、5d呈阳性,CollagenⅡ的Western Blot 9d为阳性,较对照组有显著差异;②转基因MSC经纤维蛋白体外三维培养后免疫组化和苯胺蓝染色呈阳性,电镜显示细胞生长良好,有基质合成;③转基因修复组各时期大体、组织形态学和组织化学成份均显著优于其它两组,12W时力学和组织学已接近正常关节软骨。

A study on the anatomical structure and the barbloin's store place of Pulsatilla chinensis of different growth lengths by anatomy and barbloin histochemical localization shows that multicellular nodulos, secretory cavities and secretory ducts in phelloderm and secondary phloem are the primary place of the barbloin substances and the major structural characteristics, wide or narrow of the secretory ducts is related to the growth length, 4 to 5 years old reaches maximum.

利用解剖学和组织化学定位方法对不同生长龄白头翁根的解剖结构和皂苷的储藏场所进行了研究。结果表明:栓内层和次生韧皮部中的分泌细胞团、分泌腔和分泌道是白头翁根的主要结构特征,是皂苷类物质的主要贮存场所;而分泌腔和分泌道的数量及分泌道的宽窄与生长年限相关,4~5年生达到最大值。

Fig.4 Photomicrograph of the immunocytochemical staining of TH in the brain section of the PD rat model after transplantation.

图4 移植后PD大鼠模型脑切片。TH 免疫解答细胞男友化学染色。

Fig.6 Photomicrograph of the immunocytochemical staining of GFAP in the brain section of the PD rat model after transplantation.

图6 移植后PD大鼠模型脑切片GFAP 免疫解答细胞男友化学染色。

In order to overcome the shortcomings of acellular dermal matrix used as tissue engineering scaffold material, such as bad permeability, low degradation speed, and strong immunogenicity, a new type of collagen scaffold was prepared by applying various chemical, biological and physical comprehensive methods to deal with pigskin. The microstructure of the scaffold was observed under photomicroscope and scanning electron microscopy, and the in vitro degradation time, permeability, tensile-strength, porosity, shrinkage temperature were tested.

为了克服猪脱细胞真皮基质作为组织工程支架材料渗透性差、降解速度过慢、免疫原性较强等缺点,采用多种化学、生物与物理综合方法处理猪皮制备了一种新型天然胶原支架材料,通过光学显微镜、扫描电镜观察以及体外降解时间、透水汽性、拉伸强度、孔隙率、收缩温度等的测定,对其性能进行了研究。

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