细胞化学

Cells were collected to undergo HE staining and immunocytochemistry reaction against Thy-1 to identify RGCs. Axons length of retinal neurons and RGCs were measured by microruler under microscope respectively to evaluate the neurotropic effects of B vitamins in improving the regeneration and growth of axons for retinal neurons and RGCs. Finally, we observed the reaction of retinal neurons when exposed to the insult of glucose deprivation. The neuroprotection of B vitamins were also evaluated at the same time.

对B族维生素的神经营养和保护作用的研究过程分为三个阶段，首先确定维生素B族对视网膜神经元是否有神经营养作用，并根据细胞活力找出最佳有效作用浓度；然后在接种细胞的即刻用各种维生素的最佳有效作用浓度作用于视网膜神经元，并在不同时间终止培养，收集细胞，HE染色和RGCs的免疫细胞化学染色，分别测量视网膜神经元和RGCs的轴突长度，评价维生素B族是否有促进视网膜神经元和RGCs轴突再生伸长的神经营养作用；最后，观察视网膜神经元对低糖损伤的反应，及维生素B族对受损的细胞是否有保护作用。

RESULTS: High-purity ADSCs were acquired successfully. The ADSCs were induced into osteoplastic differentiation, and displayed typical adipocytes changes 7 days after induction. the cells turned to slender fusiform and mixed together, which showed positive PPARγ immunohistochemical staining and the lipid droplet was stained orange-red by oil red staining.

结果：①成功获得纯度较高的脂肪基质细胞，成功诱导其脂向分化，诱导培养后7 d观察到细胞形态学上表现出典型的脂肪细胞改变：细胞相互融合，成片生长，变为细长纺锤形；PPARγ免疫细胞化学染色呈阳性；油红O染色脂滴被染成橙红色。

Methods : MTr assay was used to examine the effect of Sorbaria sorbifolia on the proliferation of HepG-2 cell and flow cytometry was used for the cell cycle distribution.

采用MTT比色法观察珍珠梅提取物抑制肝癌HepG-2细胞增殖；采用流式细胞仪检测其对HepG-2细胞周期分布的影响，同时结合倒置显微镜、HE染色、透射电镜及免疫细胞化学方法检测凋亡相关基因bcl-2、p53的蛋白表达，观察其诱导肝癌HepG-2细胞凋亡的作用。

There were five groups in the examination of cellular levelK562 group,K562/A02 group,K562+ADM group,K562/A02+ADM group and K562/A02+TTD+ADM group）.The non-cytotoxicity doses to cell lines K562 and K562/A02 of TTD were got by MTT assay.Using flow cytometry (FCM assay to examine the intracellular ADM concentration.There were three groups in the examination of genic,zymologic and protein levelsK562 group,K562/A02 group and K562/A02+TTD group）.The mRNA expression of MDR was measured by fluorescent quantitative reverse transcriptase polymerase chain reaction(RT-PCR.The expression levels of glutathione-S-transferase and topoisomerase Ⅱ were determined by immunohistochemical technique.

细胞水平检测实验分5组（K562组、K562/A02组、K562+ADM组、K562/A02+ADM组和K562/A02+TTD+ADM组），采用MTT法检测TTD对K562和K562/A02细胞的非细胞毒性剂量，流式细胞术检测细胞内阿霉素的浓度，基因、酶学、蛋白水平检测实验分3组（K562组、K562/A02组和K562/A02+TTD组），采用RT-PCR法检测mdr1 mRNA的表达，免疫细胞化学方法检测谷胱甘肽S转移酶π和拓扑异构酶Ⅱ的表达水平，Western-blotting法检测P-糖蛋白和bcl-2表达。

MATERAIL AND METHODS: The cell proliferation inhibition was measured by (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromid,MTT) colorimetric assay. Morphological changes of EJ cells were observed by fluorescence staining of Hoechst 33258. Cell cycle and apoptosis were analyzed by flow cytometry.

材料与方法：四甲基偶氮唑蓝(3-[4，5-dimethylthiazol-2-yl]-2，5 diphenyl tetrazolium bromid，MTT)法检测EJ细胞增殖活力；荧光染色观察凋亡细胞的形态学变化；流式细胞术分析细胞周期及细胞凋亡；免疫细胞化学染色观察caspase-3 蛋白的表达。

Ca2+-ATPase and acid phosphatase of fiber in Phyllostachys edulis culms during secondary wall formation were studied. Early, intact double karyotheca remained in fiber nucleus, while nuclear chromatin agglutinated.

利用显微和细胞化学方法，对毛竹茎秆纤维次生壁形成过程中超微结构变化以及ATP酶、Ca2+-ATPase和酸性磷酸酶的超微细胞化学定位进行了研究。

Most 5-HT cells appeared in the base part of gland in the rat, while in the human they were mainly located in the intercellular substance and a few scatted in the glandular epithelium.

方法采用免疫细胞化学方法，检测人和大鼠胃窦部粘膜内生长抑素细胞、胃泌素细胞、5-羟色胺细胞（5-HT细胞）、嗜铬粒素A细胞的分布。

Methods:Based on the model of human umbilical vein endothelial cell,this study adopted Rose Bengal Stain, cell ELISA, immunocytochemistry techniques to investigate the effect of berberine on lymphocyte-endothelium adhesion and the molecular mechanism.

以小檗碱处理人淋巴细胞或人脐静脉内皮细胞后，用蛋白染料染色法研究对淋巴细胞与内皮细胞粘附的作用，用细胞ELISA、免疫细胞化学染色法研究对细胞表面粘附分子表达的影响。

The inhibition of SPG-Rg3 on the growth of MCF-7 cells was detected by MTT assay,IC50 was calculated to obtain the effective concentration; flow cytometry was used to observe the cell cycle of MCF-7 cells; AO/EB fluorescence double-dye technology was performed to observe the apoptosis of cells in morphology,immunocellularchemistry and RT-PCR were utilized to investigate the apoptosis of MCF-7 cells and its relationship with caspase-8 from protein level and molecule level.

采用MTT法观察SPG-Rg3对MCF-7细胞生长的抑制作用，并计算出IC50，依据IC50值确定SPG-Rg3的有效浓度；流式细胞术检测SPG-Rg3作用后MCF-7细胞周期的变化；AO/EB双染从形态学上观察SPG-Rg3对MCF-7细胞凋亡的作用；免疫细胞化学染色和RT-PCR技术分别从蛋白水平和分子水平上检测SPG-Rg3对MCF-7细胞的诱导凋亡作用及其与caspase-8基因的关系。

After renal cortexes were Grinded and digested with 0.25%tryspase.The cell types of epithelial cell were identified by immunocytochemistry.The injury model of rats, renal tubular epithelial cells were established by using gentamicin.And observed the role of bFGF in renal tubular epithelial cell.

通过对大鼠肾皮质研磨、过网的方法，并以0.25%胰蛋白酶消化，以免疫细胞化学方法鉴定体外培养的肾小管上皮细胞(抗Cytokeratin 18抗体)，以庆大霉素对培养的肾小管上皮细胞建立损害作用模型，然后观察碱性成纤维细胞生长因子对损害的肾小管上皮细胞的保护作用。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。