细胞化学

At the end of the culture, PBMCs cultured on coverslips were fixed and stained for tartrate resistant acid phosphatase, a marker of osteoclast.

对培养终末细胞作组织化学染色，检测破骨细胞特征性标志物抗酒石酸磷酸酶的表达；并以象牙磨片上虫蚀样骨吸收陷窝的形成为指标检测其生物学活性。

Immunohistochemistry were positive for CD34 in some region where the fibroblast actively proliferated, whereas fibrocytes in mature fibrous tissue were negatively stained.

CD34免疫组织化学染色显示一些增生活跃的纤维母细胞明显表达CD34，成熟的纤维组织内的纤维细胞CD34则为阴性。

Methods Fifteen healthy, female SD rats were employed in the experiment. Sensitized systematically with keyhole limpet hemocyanin, the animals were inoculated with the same antigen through cochlea basal turn into the labyrinth. Adminstrated 5-bromo-2′-deoxyuridine intraperitoneally, the rats were sacrificed and the temporal bones were harvested at 3, 7,14 day after labyrinth vaccination respectively. The frozen sections of the decalcified samples were dealt with H-E staining and immunohistohemical methods to investigate the cellular infiltration, BrdUrd and IgG positive cells in the ES.

选用SD大鼠15只，以钥孔虫戚血蓝蛋白（keyhole limpet hemocyanin，KLH）全身免疫后，经耳蜗底回钻孔以相同抗原进行内耳免疫，然后分别在内耳免疫后3、7和14 d腹腔注射溴脱氧尿嘧啶核苷（bromodeoxyuridine，BrdUrd）后处死动物，取颞骨经组织学处理制作冰冻切片，用免疫组织化学技术观察内淋巴囊的细胞浸润、增殖和IgG细胞的分布状况。

Insulin cells (insulin immunoeractive cells, B-cells) in the splenic lobi of the pancreas were labelled from 0 to 365 days old shao ducks with ABC immunohistochemical method.

用ABC免疫组织化学方法，标记0～365日龄绍鸭胰腺脾叶内胰岛素免疫反应细胞，用显微测微尺测量B细胞胰岛的面积，作统计学分析。

In both cases, when they grew the cells in the presence of a chemical that infiltrates newly synthesised DNA, they could see that the unlabelled template strands of DNA went into one daughter cell and the new strands went to the other.

他们在培养基中添加能渗入新合成DNA的化学试剂，结果，两种情况下都观察到未标记的模板DNA链进入了一个子细胞，而新合成的DNA链进入了另一个子细胞。

Methods Apoptosis Index was assessed with TUNEL technique,and immunohistochemical stainings of MDM2 and Pgp were performed in 83 cases of the carcinomas and 11 "normal" bladder mncosa.

采用免疫组织化学方法及TUNEL法检测11例&正常膀胱黏膜&和83例膀胱移行细胞癌中MDM2、P53基因的表达及细胞凋亡指数。

11 guinea pigs which were successfully induced myopias were extracted eyeballs, and the scleras near posterior poles were observed by immunohistochemistry methods. The positive rates of TGF-β〓 and bFGF in scleral fibroblast cytoplasma and the area percentageratio of positive cells were calculated and contrasted to controlled eyes.

将透镜诱导近视成功的11只豚鼠处死，摘除眼球，取后极部巩膜分别进行TGF-β〓和bFGF的免疫组织化学染色，观察它们在巩膜组织成纤维细胞胞浆中中表达的阳性率和阳性细胞所占面积的百分比，并与对照眼进行比较。

11 guinea pigs which were successfully induced myopias were extracted eyeballs, and the scleras near posterior poles were observed by immunohistochemistry methods. The positive rates of MMP-2 and TIMP-2 in scleral fibroblast cytoplasma and the area percentageratio of positive cells were calculated and contrasted to controlled eyes.

将透镜诱导近视成功的11只豚鼠处死，摘除眼球，取后极部巩膜分别进行MMP-2和TIMP-2的免疫组织化学染色，观察它们在巩膜组织成纤维细胞胞浆中表达的阳性率和阳性细胞所占面积的百分比，并与对照眼进行比较。

Kir4.1 and Kir7.1 acomplished by ABC immunohistochemistry in monkey and human retinae got the nearly same results as bovine .

ABC免疫组织化学显示Kir2.1和Kir4.1分布在猴和人视网膜 M沮ler细胞的不同部位，而Kir7.1分布于人和猴的RPE细胞的游离面及其突起。

Fever, endogenous pyrogens (IL-1, IL-2, TNF, IFN), and oxygen free radicals in inflammation can all induce the synthesis of HSPs in cells.

已有报道表明，某些病毒和化学物质转化的细胞，肿瘤细胞系以及人类肿瘤细胞中HSPs的表达增加。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。