细胞化学

Methods Immunohistochemical and morphometrical analysis methods were performed on vital skin wounds. Results In normal and injury mouse skin IL-10 immunoreactivity was observed in epidermal cells.

方法应用免疫组织化学技术和形态计量法，对小鼠不同时程皮肤切创组织中IL-10不同表达部位（表皮细胞及表皮下组织的浸润细胞）的变化进行研究。

Methods A total of 53 SRE specimens and 8 nomal nephridial tissues were obtained. Immunohistochemistry method was used to test protein expression of survivin. Semi-quantitative RT-PCR was used to measure survivin mRNA levels.

收集53例肾透明细胞癌组织及8例正常肾组织，以免疫组织化学方法检测survivin蛋白表达；半定量RT-PCR方法，检测肾透明细胞癌中survivin mRNA表达水平，与正常肾组织进行比较分析。

METHODS: The corneal endothelial cells of cats were primarily cultured, and neurone specific enolase immunocytochemistry staining was performed.

猫角膜内皮细胞原代培养，行NSE免疫组织化学染色鉴定细胞的类型、纯度及生理特性。

Results There was significant negative correlation between the Nogo-A expression and the morphological change of tumor tissues according to immunohistochemistry. Western-blot analysis also indicated that the gray value of Nogo-A protein band in the oligodendroglioma group was significantly higher than that in the anaplastic oligodendroglioma group.

结果 免疫组织化学结果显示，Nogo-A免疫阳性染色的光密度值与肿瘤细胞核分裂、核异型比例呈明显的负相关，对蛋白印迹试验结果进行光密度分析也发现少突胶质细胞瘤中的Nogo-A蛋白条带灰度值明显高于间变型少突胶质细胞瘤中的灰度值。

From its introduction, oncological chemotherapy has been encumbered by its poor selectivity because most antiproliferative drugs are toxic not only to tumor cells but also to important populations of the body's non-neoplastic cells.

由于它的介绍， oncological化学疗法由它的劣质的选择性妨害了，大多数antiproliferative 药不仅对瘤细胞而且对身体非新生物的细胞的重要的人口是有毒的。

Osteogenetic role palyed by human foreskin fibrolasts cultured in vitro.

通过组织化学方法，扫描电镜观察证明人皮肤成纤维细胞同家兔皮肤成纤维细胞一样，在体外培养中可以成骨。

In this study, the ovarial cancer stem cells were successfully obtained through the suspension culture of the ovarian cancer 0VCAR3 cell line in serum-free media. The stem cells formed spherical colonies, called "ovarispheres", and showed anchorage-independent ability. Furthermore, the bionomics and morphology of the stem cells were observed, and the expression difference of the tumor stem cells marker-ABCG2 between ovarispheres and anchorage-dependent cells was identified by using RT-PCR and Immunocytochemistry methods.

本研究通过无血清悬浮培养人卵巢癌细胞系OVCAR3，获得具有非锚着依赖能力的肿瘤干细胞球，观察其生物学特性及形态学的变化，并采用半定量逆转录—聚合酶链反应及细胞免疫化学的方法鉴定肿瘤干细胞标志ABCG2在贴壁细胞及肿瘤干细胞球中的表达差异。

On the basis of light microscopic observation the immunohistochemical examination of CK,TG,NSE,vimentin and calcitonin was used to observe 9 cases of oxyphilic cell adenoma of the thyroid.

观察9例甲状腺嗜酸细胞腺瘤，在光镜观察的基础上，用免疫组织化学方法检测细胞角蛋白、甲状腺球蛋白、神经元特异性烯醇化酶、波形蛋白和降钙素。

Objective To investigate the expression and significance of Ki67,cytotoxic proteinsTIAI,GranzymeB in nasal lymphoma.Methods Paraffin-embedded tissues of34cases of nasal NK/T-cell lymˉphoma,8midline B-cell lymphomawere studied using immunohistochemical method.

目的 探讨核抗原Ki67和细胞毒蛋白TIAI、GranzymeB在鼻腔淋巴瘤的表达及意义方法以NK/T淋巴瘤34例石蜡组织标本研究对象，同部位B细胞淋巴瘤8例为对照，行免疫组织化学染色。

Intracytoplasmic sperm injection, which is a new technique of embryo engineering of domestic animals, can solve the problem of porcine polyspermy.

用GFP基因转染的精子注射后化学激活的ICSI卵母细胞基因表达率显著高于电激活处理的ICSI卵母细胞的基因表达率。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。