细胞化学

Immunocytochemistry method was used to demonstrate the expression of glial fibrillary acidic protein.

用塑料吸头划刮培养皿，制造机械损伤的模型，继续培养至72 h，分别取出不同时间点的细胞进行胶质细胞增生情况的观察和免疫组织化学染色，对胶质原纤维酸性蛋白表达产物光密度进行图像分析。

Many today yearn for a world where we could know the levels of all the proteins in a cell to finally functionate the cell—as if knowing all the elements allows one to understand all chemical structures—NOT.

今天，许多人渴望研究细胞内各种蛋白质的表达水平，认为这样就能了解细胞的功能--就像知道各元素就能理解化学分子那样--这是不可能的。

Results The results showed that the number of mast cells around the periphery of gingival cancer tissue increased significantly.

结果 在牙龈癌周围组织中肥大细胞显著增多，在组织化学上与正常牙龈组织的肥大细胞不同，不含肝素。

Methods The specimens which were identified as osteosarcoma were cultured primarily in vitro. The surviving cells underwent morphological observation, histochemical staining, isoenzyme pedigree determination, cell cycling analysis, karyotype analysis and heterotransplantation.

采用原代组织块培养法对成骨肉瘤手术标本进行培养，对存活细胞进行形态学观察、组织化学染色、乳酸脱氢酶同工酶谱、细胞周期检查、核型分析、异种移植。

Results:17 rats were confirmed successful transplantation by MRI,the capacity of the tumor was 88.40±7.62mm3 on 8th day, 986.80±114.46mm3 on 16th day,the volume growth was 8-12 folds;on 8th day following transplantation,the tumor tissue was gray in color with fish homogen state without interior necrosis, the tumor cells were nest-distributed by HE staining,large dense staining of nucleus with manifest heteromorphism,tumor microvessel count increased by immunohistochemistry with buffy yellow staining, VEGF high expressed in the tumor cells with buffy yellow granule shape.

结果：17只大鼠经MRI检查证实均种植成功，肿瘤体积第8天为88.40±7.62mm3，第16天为986.80±114.46mm3，体积增长8-12倍之间；种植第8天，肿瘤组织呈灰白色、鱼肉均质状，内部未见坏死，HE染色肿瘤细胞呈巢状分布，胞核大浓染，异型性明显；免疫组织化学示肿瘤微血管数目较多，呈棕黄色染色，肿瘤细胞VEGF高表达，呈棕黄色细颗粒状。

Ovine immatured oocytes, matured oocytes and embryos from 2-cell stage to blastocyst produced in vitro were recovered, and RT-PCR was used to semi-quantify mRNA expression. Proteins of Cx43 and Cx45 were detected by using a combination of immunocytochemistry and Laser Scanning Confocal Microscope.

收集绵羊未成熟和成熟卵母细胞以及体外受精早期胚胎各发育阶段的卵裂球细胞，通过RT-PCR半定量检测Cx43和Cx45基因的mRNA表达量；通过免疫组织化学方法结合激光扫描共聚焦显微镜检测Cx43和Cx45蛋白在绵羊体外受精的早期胚胎发育过程中的表达情况及表达区域。

Several anti-tumor cell lines and carbonic anhydrase Ⅱ model were established. More than 10000 compounds and extracts from plants were screened using these models and about 10 compounds showed strong inhibitory activities. Two species of plants Inula helianthus-aquatica and Saussurea deltoidea were chosen to investigate chemical and bioactivity constituents based on the results of screening of extracts and fractions. 58 compounds were isolated and identified from these two plants, 6 compounds were found to be new and 14 compounds showed anti-tumor activities. We also studied the anti-tumor mechanism of the compound bigelovin isolated from Inula helianthus-aquatica. Bigelovin was found with apoptotic activity against cell line U266 by lowering the amount of important transcription factor E2F-1 which inhibits the cell cycle at G0/G1 stage. Docking study on 7 natural new phenolic inhibitors of CA Ⅱ and 3D-QSAR study on 61 sulfonamide inhibitors of CA Ⅱwere also investigated. The calculated results were in good correlation with experimental results.

基于以上两种重要疾病和研究组方向，本论文工作开展了多个肿瘤细胞株和碳酸酐酶Ⅱ靶标模型的建立和筛选工作，累计筛选1万多个/次样品，发现10余个活性较强化合物；从粗提取物筛选结果中选取两种抗肿瘤活性强的菊科植物水朝阳旋覆花(Inula helianthus-aquatica)和三角叶凤毛菊进行了系统化学和活性成分研究，分离鉴定了58个化合物，发现6个新化合物和14个抗肿瘤活性化合物；通过合作对水朝阳旋覆花中活性较强的bigelovin进行了抗肿瘤作用机制研究，发现它可能是通过降低G2期重要的转录因子E2F-1，致使细胞周期阻滞在G0/G1期，引起多发性骨髓瘤U266细胞凋亡；开展了7个新的天然酚类CAⅡ抑制剂的对接研究和61个合成的系列磺酰胺类CAⅡ抑制剂的3D-QSAR分析，结果表明计算与实验一致。

These free radicals come from the metaboly of food and breath.

人体的每个细胞每天受到约10000次的自由基攻击，这些自由基来源于食物和呼吸的代谢，自由基的攻击方式严格遵循量子与正态分布原理，攻击的损伤程度取决于细胞营养状况、人体酸碱度等物理、化学状况。

The effects of combined NET-OEN on micronuclear frequencies of polychromatic erythrocytes of bone marrow and fetal liver blood were observed in mouse.

目前用哺乳动物骨髓细胞微核出现率作为检测化学物质的细胞遗传学效应的简便方法〔l~。1，已日益引起毒理学家们的重视。

METHODS:The micronucleus test of Vicia faba root tip cell was used to detect the water quality,and to survey the chemical oxygen demand.The micronuclear rates and Pollution Index were determined and the F-test was used to evaluate the difference in micronuclear rates among different samples.

材料与方法：用蚕豆根尖细胞微核技术对各采样点水样进行监测，并对各水样进行化学需氧量(chemical oxygen demand，CODcr)的测定，统计分析各采样点水样的蚕豆根尖细胞微核千分率及污染指数。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。