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细胞化学

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Hemolymph of Helicoverpa armigera and Spodoptera exigua inhibit apoptosis induced by actinomycin D in this study.

本文研究了甜菜夜蛾和棉铃虫血淋巴抑制化学物质细胞放线菌素D诱导的细胞凋亡的作用。

P1 BMSCs, P3 CCs, and P3 ADAS were implanted into collagen/hyaluronic acid scaffold at different concentrations of 10%, 20%, 50%, 80%, and 100%, and the cells were then three-dimensionally cultured in serum-free culture media. Two weeks later, SO staining and immunohistochemical staining were employed to observe the synthesis between of chondroitin sulfate and type II collagen.

取P1骨髓间充质干细胞,P3软骨细胞和P3脂肪源性成熟基质细胞种植入不同比例(10%,20%,50%,80%,100%)的胶原/透明质酸支架中,在无血清培养液中三维培养。2周后,用SO染色和免疫组织化学染色分析,观察硫酸软骨素和Ⅱ型胶原合成情况。

Rat in Group B was set an air bladder which was empty and not perfused water. Water of 0.6 ml, 0.8 ml, 1.0 ml, 1.2 ml, 1.4 ml and 1.5 ml was perfused respectively into the air bladders which were set in the colon of rats in group C, D, E, F, G and H. Then the changes of ethology of the rats were recorded in interval of an hour.

应用疼痛行为学的观察方法,观察1h各组动物的行为学改变,并采用免疫荧光化学的方法,观察大鼠DCN中神经元被激活的标志c-fos及胶质细胞的胶质原纤维酸性蛋白(glial fibrillary acidic protein, GFAP)和小胶质细胞特异性补体OX42的表达情况,空白对照组是在疼痛高峰点(1.2 ml)处死取材。

This finding corresponded to the immunohistochemical data showing the presence of phosphorylated forms of p38, c-Jun N-terminal kinase, and extracellular signal-regulated kinase during ameloblast differentiation.

这个发现与免疫组织化学数据相符,显示在成釉细胞的分化过程中,p38磷酸化形式,c-Jun氨基端激酶以及细胞外信号调节激酶均有表达。

AIM: To explore the effect of anthocyanin on cholesterol efflux and elucidate its possible molecular mechanism.

目的:研究植物化学物质花青素对小鼠巨噬泡沫细胞胆固醇外流的影响,探讨花青素促进巨噬泡沫细胞胆固醇外流的分子机制。

Methods Tissue arrayer was used to create a 252-dot tissue microarray, consisting of 84 cases of malignant lymphomas arising in esophagus, stomach, intestines, thyroid, saliva gland , orbital cavity and lung, Seven cases of diffuse large B cell lymphomas,9 adenocarcinomas, 1 neuroendocrine tumor,1 squamous carcinoma, 6 T-cell lymphomas and 18 reactive lymphoid nodes were also included as control in the microarray.

建立包含84例MALT型淋巴瘤、7例弥漫性大B细胞淋巴瘤、9例腺癌、1例神经内分泌肿瘤、1例鳞癌、6例T细胞性非何杰金淋巴瘤和18例正常淋巴结共252点阵的组织芯片,应用免疫组织化学检测Ki-67、TopoⅡα、p53、Bcl-2、Bax、C-myc和Bcl-10在MALT型淋巴瘤中的表达情况。

Lysosome is a reservoir that contains digestive enzymes. These kind of enzymes can break down worn-out organelles and digest foreign substances.

溶J体是一个具有消化能力化学物的储存库,这些化学物能分解损耗了的细胞器和消化外来的物质。

Methods Cytological and chemical examinations of the peripheral venous blood were conducted in ILT peafowls before and after treatment by daily oral therapy with Chinese medicine S-anti-virus composite mixture at the dose of2g.d.

摘 要]目的探讨中药复方S-抗毒合剂治疗传染性喉气管炎后血液细胞和血液化学的变化方法以孔雀实验对象,将中药按每日每只2g拌饲料投喂ILT孔雀进行治疗,并于治疗后取翅静脉血做血细胞和化学检测。

MAIN OUTCOME MEASURES: Morphology change of spinal cord was detected by light microscope and electronmicroscope, secreted brain-derived neurotrophic factor was measured by immunohistochemistry and PT-PCR, and the combine behavioral score was used to assess the functional recovery of spinal cord. RESULTS: Immunohistochemistry result showed that OECs transplantation could partly reduce the number of apoptosis cells in spinal gray matter, delay cells reduction in spinal white matter, and accelerate the medullary sheath repair and regeneration.

主要观察指标:应用光学显微镜、电子显微镜观察脊髓形态的变化,采用免疫组织化学、PT-PCR 的方法检测脊髓组织脑源性神经营养因子的分泌,以改良的Gale联合行为评分法对脊髓功能进行评定,结果:免疫组织化学检测显示,嗅鞘细胞移植能部分改善大鼠脊髓灰质神经细胞的凋亡程度,延缓白质神经纤维的减少,促进髓鞘的修复与再生。

Each 1 μL site of spinal cord receiving 109 /L OECs at the speed of 1μL/ min. MAIN OUTCOME MEASURES: Morphology change of spinal cord was detected by light microscope and electronmicroscope, secreted brain-derived neurotrophic factor was measured by immunohistochemistry and PT-PCR, and the combine behavioral score was used to assess the functional recovery of spinal cord. RESULTS: Immunohistochemistry result showed that OECs transplantation could partly reduce the number of apoptosis cells in spinal gray matter, delay cells reduction in spinal white matter, and accelerate the medullary sheath repair and regeneration.

主要观察指标:应用光学显微镜、电子显微镜观察脊髓形态的变化,采用免疫组织化学、PT-PCR 的方法检测脊髓组织脑源性神经营养因子的分泌,以改良的Gale联合行为评分法对脊髓功能进行评定,结果:免疫组织化学检测显示,嗅鞘细胞移植能部分改善大鼠脊髓灰质神经细胞的凋亡程度,延缓白质神经纤维的减少,促进髓鞘的修复与再生。

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