细胞化学

MTT method was used to detect the proliferation of the cells. Cell alkaline phosphatase activity and expression of collagen type I were assessed.

另以组织化学和免疫组织化学方法检测传代培养的SD大鼠骨髓基质成骨细胞在PMMA驻极体表面的增殖和分化情况。

Objective: To observe the effect of decalcification on mast cell histochemical and immunohistochemical staining in rat cranial bone samples.

目的：探讨脱钙对鼠颅骨标本中肥大细胞组织化学与免疫组织化学染色影响。

Detecting each marker and cmparison the location of sequential tissuesection could determine the area loceation relationship of the markers, but it is difficulte to determine their correlation intra-cells exactly. No report of detecting virus genome and antigenes on a one section with triple staning techniques exists in the lit erature. in this report. by combining immunohistochemistry and in situ hybridization (with dioxigenin labelled HBV DNA probe), a triple staining technigce was established. With this technic, on one formalin-fixed and paraffin embeded section, HBV DNA, HBsAg and HBcAg were determinated one after the other.

各个细胞内病毒的复制和表达水平以及与肝细胞病变的关系，是乙型肝炎发病机理研究的一个重要方面：目前常用的在系列切片上对各标志物分别检测，比较观察的方法，一般只能大致了解各待检物在某一区域的定位关系，很难准确地了解它们在同一细胞内的关系：国内外尚未见有在同一张切片上三重标记染色检测病毒核酸及其多种抗原的报道：本文结合免疫组织化学ABC法及地高辛配基标记HBV DNA探针原位杂交，建立三重标记染色技术：在一张福尔马林固定石蜡包埋肝组织切片上先后检测，同时显示HBV DNA、HBcAg和HBsAg。

After treated in 4% paraformaldehyde,we applied Hematoxylin-Eosin and Nissl staining to exhibit the structure of the retinae and ganglion cells respectively,and also we used immunohistochemical ABC method to display the distribution of GFAP-immunoreactive cells that were specifically classified as the astrocytes.

E 染色以显示视网膜结构，Nissl染色显示神经节细胞，免疫组织化学ABC法染色以显示星形胶质细胞特征性标志物胶质纤维酸性蛋白的阳性反应细胞的分布。

The rationals of anti-angiogenesis are the following: the therapeutic target is tumor endothelial cells which are more genetically stable, more homogeneous, and with lower mutation rate; the anti-angiogenesis drugs can contact endothelial cells directly, with higher potency and lower drug barriers and side effects; the aim of anti-angiogenesis therapy is to block the oxygen and nutrition supply from vessels, instead of killing tumor r ells per se; the aim of anti-angiogenesis is to reduce tumor size instead of eradicate tumor which are different from traditional chemotherapy.

使用抗血管新生疗法根据的理论基础为以肿瘤血管的内皮细胞为治疗药物的攻击标的；肿瘤血管的内皮细胞基因比较稳定、同质性高、而且突变率低；投与的药物可以直接接触内皮细胞，减少投与药物屏障，因此增加有效投与药物的能力及减少药物副作用；以截断供应肿瘤氧气养分的血管为目标：以减小肿瘤的体积大小为目的，与传统化学疗法以根除肿瘤的目标不同。

Rabbit digestive tract were investigated by the method of immunohistochemical technique and histochemical technique (toluidine blue or neutral red staining). There was immunoreactive positive reaction in rabbit digestive tract except in esophagus part.

利用免疫组化技术和组织化学技术，对家兔消化道5-HT免疫反应阳性细胞、肥大细胞进行了研究，并通过邻片法比较探讨了2种细胞的对应关系。

This experiment unified flow cytometry, the monoclonal antibody technology, the cell fluorescence chemistry technology and unilized the union principle of antigen and antibody to examine T lymphocyte subgroup, the B lymphocyte change in circumference blood of the mice through the flow cytometry; At the same time, the agarose gelatin electrophoresis examined bovine colostrum effect on programmed cell death of thymus.

本实验正是将流式细胞术、单克隆抗体技术、细胞荧光化学技术相结合，并利用抗原抗体结合原理，采用流式细胞仪检测了小鼠外周血的T淋巴细胞亚群和B淋巴细胞；同时采用琼脂糖凝胶电泳技术检测了牛初乳对小鼠胸腺细胞凋亡的影响。

Theoretical classes focus on the conceptions involving cell biology, the ultrastructures, chemical composition and function of organelles in cell, the mechanisms of biochemical action and the research methods as well.

理论课教学内容重点讲授细胞生物学有关的基本概念、细胞内各种机构及细胞器的亚显微结构、化学组成和功能作用，介绍重要生化活动的分子机制及本学科的主要研究方法。

The method falls through collect form experiment and naked rat to send tumour experiment to observe vitamin A and 4HPR proliferous to Hela cell influence; passes law of electrophoresis of gel of electron microscope and candy of cellular DNA agaragar to observe vitamin A and 4HPR to die to Hela cell wither the influence of configuration and biochemical feature.

方法通过集落形成试验和裸鼠致瘤试验观察维生素A和4HPR对Hela细胞增殖的影响；通过电子显微镜及细胞DNA琼脂糖凝胶电泳法观察维生素A和4HPR对Hela细胞凋亡形态的影响及生物化学特征。

Methods The rat thoracic duct endothelia were cultured and the morphology observed and identified by TEM and Factor Ⅷ-related antigen immunohistochemically. Wistar rats were used to make the animal model of primary colorectal cancer, the cancer type were appraised by HE staining and PAS Reaction. The expression of VEGF-C and its receptor Flt-4 and ICAM-1 in the cancer tissues were carried through immunohistochemistry staining and In situ hybridization.

通过体外培养大鼠胸导管内皮细胞和构建大鼠原发性大肠癌动物模型，观察内皮细胞的形态和生长状态，用透射电镜和Ⅷ因子相关抗原进行鉴定，用HE染色和过碘酸雪夫氏反应区别癌组织的类型和期别，用免疫组织化学方法和原位杂交方法研究内皮细胞和大肠癌组织的VEGF-C及其受体Flt-4和ICAM-1的表达。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。