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细胞化学

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Cell cycle and surface antigen were detected with flow cytometry. Adipogenic differentiation and osteogenic differentiationwere tested by immunohistochemistry.

流式细胞仪测定细胞周期及细胞表型,免疫组织化学染色检测其体外诱导成脂肪和成骨分化的能力。

Eight kittens were deduced amblyopia by monocular chronic atropinization; four (2 normal and 2 amblyopia) kittens were perused in every three weeks. Changes in number and optical density of immunopositive neurons of the expression of VIP-protin and VIP-mRNA in the visual cortex area 17 were analyzed with immuno-cytochemistry, using the technique of in situ hybridization and SABC.

在用药后3周、6周、9周、11周分别每组中各处死两只猫,分离出视皮质17区行HE染色观察神经元形态、细胞数目以及电镜观察超微结构变化,VIP-mRNA原位杂交和VIP免疫组织化学染色观察免疫阳性细胞数以及阳性细胞平均光密度,并分别用光镜下计数和计算机图形分析软件得出结果。

All these cytokines are involved in transendothelial cell migration, either by inducing adhesion molecules or via direct chemotactic effects.

所有这些细胞素通过诱导黏附细胞或直接的化学趋化性,卷入经上皮细胞的移行。

In the present study changes of NOS positive cells after injection of LPS and apoptotic ependymal cells, the relationship between expression of immediate early gene c-fos and astrocytes, and comparison of changes between SFO and rest regions of the brain in different time-points were investigated with NADPH-d histochemistry, apoptosis in SITU detection of TUNEL, TEM and SEM, fluorescein double labeling of immunohistochemistry, and laser scanning confocal microscope .

为了证实穹窿下器在脑内免疫调节系统中的作用,在本研究当中,应用了NADPH-d的组化方法、TUNEL末端标记法、扫描及透射电镜方法、免疫组织化学的荧光双标法及激光扫描共聚焦显微镜技术,来研究在腹腔内给予大肠杆菌内毒素后,脑内的一氧化氮合酶细胞的变化、室管膜细胞的凋亡、早期即刻基因c-fos的表达与星形胶质细胞之间的关系,以及穹窿下器与其它脑区相比较时的特异性改变。

In the present study changes of NOS positive cells after injection of LPS and apoptotic ependymal cells, the relationship between expression of immediate early gene c-fos and astrocytes, and comparison of changes between SFO and rest regions of the brain in different time-points were investigated with NADPH-d histochemistry, apoptosis in SITU detection of TUNEL, TEM and SEM, fluorescein double labeling of immunohistochemistry, and laser scanning confocal microscope.

为了证实穹窿下器在脑内免疫调节系统中的作用,在本研究当中,应用了NADPH刁的组化方法、TUNEL末端标记法、扫描及透射电镜方法、免疫组织化学的荧光双标法及激光扫描共聚焦显微镜技术,来研究在腹腔内给予大肠杆菌内毒素后,脑内的一氧化氮合酶细胞的变化、室管膜细胞的凋亡、早期即刻基因(immediate early genes沁-fos的表达与星形胶质细胞之间的关系,以及穹窿下器与其它脑区相比较时的特异性改变。

After the culture for 14 days, the proliferated fusiform cells began to increase in a great amount.

诱导后第9天,收集细胞,行MTT比色法、流式细胞仪和免疫组织化学检测鉴定细胞。

Removed veins research demonstrate the main reason of transplanted vessel stenosis is the hyperplasic of endothelium. Which is a popular reaction when set vein into arteral circulation. It is caused by the transmission of smooth muscle cell to endothelium and proliferate and release extracellular matrix. These are two characteristics in pathology erg middle layer of sue proliferates and migrate to endothelium in early and excel of end thulium aggregate in cater stage. The colic and the motivation of end thulium hyper is the hyper of sac.

在mRNA水平,随着移植术后时间的推移,ECE的表达逐渐增高,手术后1W-2W达到高峰2W后表达逐渐下降,于8W左右趋于平衡,ET-1的表达与ECE的动态变化过程相似,亦于术手1W-2W达到高峰,在8W左右趋于平稳在蛋白质水平,以ET-1抗体免疫组织化学染色结果显示ET-1主要在血管内收细胞及平滑细胞胞浆中表达,尤其是中膜平滑肌细胞更为明显,呈棕黄色颗粒。

Juvenile macromastia ; mammary epithelial cell ; cell size ; proliferation marker ; immunohistochemistry stain ; Ki-67 ; Akt-1/2/3 ; STAT5a

中文关键字青春期巨乳症;乳腺上皮细胞;细胞尺寸;细胞增生分子标记;免疫组织化学染色; Ki-67 ; Akt-1/2/3 ; STAT5a

Immunohistochemically, cells in myoid/myofibroblastic areas showed positive staining for α-SMA, MSA and vimentin, but negative for desmin and CD34. Electron microscopic study displayed the presence of microfilament bundles, focal dense bodies and micropinocytic vesicles, consistent with those of myofibroblasts.

免疫组织化学标记显示肌样区域细胞表达α-平滑肌肌动蛋白和肌特异性肌动抗原,不表达CD34;电镜观察证实细胞含有质膜下微丝束、局灶性致密体及微胞饮囊泡样结构,与肌纤维母细胞相一致。

Immuno histochemical technique and histology method were used to investigate the changes of the number of somatostatin poistive cells,IgA positive and acid naphthyl acetate esterase positive cells in porcine spleen during growth.

用免疫组织化学技术研究猪脾生长发育过程中生长抑素阳性细胞、IgA阳性细胞和酸性非特异性脂酶阳性细胞数量的变化。

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