细胞化学

METHODS: Autologous whole blood was injected into the vitreous cavity of albino rabbits after canalization, thus induced experimental PVR model. Concentration of PDGF.

采用眼穿通伤+注血法制备PVR模型，进行眼底及常规组织病理学观察，ELISA法检测玻璃体中血小板源性生长因子的浓度，用免疫组织化学方法观察增生膜与血管内皮细胞表面血管细胞粘附分子-1（VCAM-1）及细胞间粘附分子-1（ICAM-1）的表达。

Methods: Introduce cytopathology experimental methods, human hepatic cancer cell line Bel-7402 co-cultured with Triptolide (10 g/mL, 5μg/mL and 0.25μg/mL), Using MTT method to test the effect of Triptolide on Bel-7402 cell proliferation, using TUNEL technique to test the effect of Triptolide on Bell-7402 cell apoptosis, immunohistochemistry staining method to test Bax and Bcl-2 protein expression.

采用细胞药理学实验方法，人肝癌细胞株Bel-7402与雷公藤甲素（10μg/mL、5μg/mL及0.25μg/mL）共培养，MTT法检测其对Bel-7402细胞增殖的影响，TUNEL技术检测其对Bel-7402细胞凋亡的影响，免疫组织化学染色法检测Bax及Bcl-2蛋白表达。

Some physicochemical therapy as well as nake hepatocytes transplantation can't correct metabolic disarrangement because of severe hepatic diseases, ALF is characteristic of more complications and easily resulting in multiple organs dysfunction , and even multiple organs failure as well as higher mortality. transplant technique of encapsulated hepatocytes can inhibit immune rejection, and quarantee hepatocytes survival, the technique provides a new therapeutic method for ALF.

由于肝脏功能较复杂，一些利用物理、化学方法及裸肝细胞移植来代替肝脏解毒功能，但不足以纠正严重的肝病所致的代谢紊乱，易致多脏器功能障碍甚至衰竭，并发症多，死亡率高；而微囊化肝细胞移植技术，既可阻断受体的免疫排斥反应，又保证移植的肝细胞在体内长期存活，为急性肝衰治疗提供新的治疗方法。

Results: The tHey and blood fat level in blood plasma and expression of MCP-1mRNAof aorta on model group is obviously higher than that of control group (P＜0.05);the result of immunohistochemistry shows that the expression of VCAM-1 and MMP-2enhanced, Bcl-2 is lower; aorta slice shows endarterium discontinuation, strippingpartly, smooth muscle cell arrange puffly and chaotic, infiltration of lipoid ofdifferent degree are observed in aorta wall, pathological changes suffusion, morexanthoma cell, plaque protrude lumens. The they and blood fat level in blood plasmaand expression of MCP-lmRNA, VCAM-1, MMP-2 of aorta on intervention group aremuch lower than that of model group (P＜0.05); Bcl-2 of intervention group isstrengthened, and the pathomorphology and immunohistochemistry of aorta showpathological changes are slighter than model group.

结果：模型组，血浆中同型半胱氨酸浓度、血脂水平、主动脉MCP-1mRNA表达显著高于正常对照组(P＜0.05)，免疫组化示主动脉内皮细胞VCAM-1、MMP-2表达增强，Bcl-2表达降低；主动脉切片发现动脉内膜不连续，部分剥脱，平滑肌细胞排列疏松、紊乱，主动脉壁均见到程度不同的脂质浸润，其病变弥漫，泡沫细胞较多，可见斑块突出管腔；干预组，血浆中同型半胱氨酸含量、血脂水平显著低于对照组和模型组(P＜0.05)，主动脉MCP-1mRNA、VCAM-1、MMP-2表达显著弱于对照组和模型组(P＜0.05)，Bcl-2表达增强(P＜0.05)，主动脉病理形态学和免疫组织化学检测示病变轻于模型组。

The method uses groovy histology and immunity to organize chemical technology, observation mars agent 6- hydroxide via celiac inject sympathetic much cling to small rat small intestine is epithelial after amine the amount of endolymph cell, cup shape cell, IgA+ oar cell, corpulent cell distributings change.

概要： 目的探究交感神经对肠道黏膜屏障的调节功能。方法采用常规组织学和免疫组织化学技术，观察经腹腔注射交感神经损毁剂6-羟多巴胺后小鼠小肠上皮内淋巴细胞、杯状细胞、IgA+浆细胞、肥大细胞的数量分布变化。

This study that observed the expression and distribution of 4 apoptotic factors, relation apoptotic factor with apoptosis, changes of T and B subgroup, and changes of molecular pathology in 14 cows suffering from enzootic bovine leucosis from several resides was detected by histochemistry, TUNEL technology, immune-fluorescent flow cytometry and so on. The aim of this experiment supplies theoretic basis for preventive and therapy of EBL, and consult dates for T lymphoid cell leucosis of human being .

本研究检查并剖检了14例临床与实验室诊断为EBL的病牛，观察分析了肿瘤发生的情况和特征，采用免疫组织化学ABC法、原位末端标记TUNEL技术和免疫荧光流式细胞检测法等技术，从EBL肿瘤细胞凋亡特性入手，围绕4种凋亡因子和几种免疫相关因子与EBL肿瘤发生的关系及其在诊治方面的应用进行了首次有价值的探索，其目的在于为防治EBL提供科学的理论依据，同时，也将为人类T细胞白血病Ⅰ。

The expression of cyclins D1 and E required for initiation of S phase were also studied by immunochemistry method.

应用流式细胞仪分析CGRP对VSMC细胞周期的影响，免疫组织化学检测细胞周期蛋白D1、E的表达。

The times of penis intromission into vagina of estrous female rats during 30 minutes observation and intracavernosal pressure during erection resulting from stimulation of the autonomic innervation of the penis were measured after 2 weeks and 2 months of the operation.

应用内皮细胞特异性标志物CD-31分子和平滑肌特异性标志物α-actin对处理后2周的大鼠阴茎海绵体组织行免疫组织化学染色，了解雄激素对大鼠阴茎组织血管内皮细胞和平滑肌细胞的影响。

A cell is not just a well-mixed container of chemicals, but an extremely intricate system of membranes, vesicles and signaling pathways. Although a number of milestones have been built though endeavor for over a century, much is still left to discover about the spatial organization and mobility of macro-molecules (such as proteins, protein complexes, nucleic acids, signaling substances, etc.) in cells, and the spatial relationships between cells in tissues.

细胞不仅是大量化学物质的容器，也是膜、泡囊和信号传导通路组成的复杂系统，尽管通过一个多世纪的努力，细胞生物学研究取得了若干重大进展，但是关于细胞内特定高分子(如蛋白质、蛋白质复合体、核酸和信号传导物质等等)的空间组织和运动规律以及组织内细胞空间关系等许多问题的机理目前仍不清楚。

This page provides some protocols about porcine brain tubulin, sea urchin sperm axonemes, rat liver cell cytosol and organelle fractions, organelle motility assay, tissue culture of PtK1 cells, basic protein chemistry and so on.

该网页提供了一些有关猪脑的微管蛋白、海胆的精子基因丝、大鼠肝脏细胞的细胞质和细胞器的级分、细胞器的运动性实验、PtK1细胞的组织培养、基本的蛋白化学等等方面的操作流程。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。