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细胞化学

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In the gravid uterus, The CKs immunolabelling were detected in glandular cell, luminal epithelial cell, traphoblast cell, endoblastic cell and allantoic cell; Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus but in endoblastic cell and some luminal epithelial cell.

对分离得到的子宫内膜基质细胞和子宫内膜腺上皮细胞进行免疫组织化学标记的结果显示在体外子宫内膜基质细胞表达泛角蛋白,子宫内膜腺上皮细胞表达波形蛋白,并且这一特性不因为传代而发生丢失。

Bone marrow mesenchymal stem cells could differentiate into osteoblasts, adipocytes and neural like cells with osteoblast inductor (β-sodium glycerophosphate, dexamethasone, vitamin C), lipoblast inductor (dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin, indometacin) and serum-free medium inductor (dimethyl sulphoxide, butylated hydroxyanisole) respectively. Osteoblast marker (alkaline phosphatase, osteocalcin mRNA, calcium node), adipocyte marker (lipid droplet, PPAR γ-2mRNA) and neural cell-like marker (nissl body, neuron specific enolase, neurofilament protein) were respectively determined by the immunohistochemical method, polymerase chain reaction and immunocytochemical method.

分别采用成骨细胞诱导剂(β-甘油磷酸钠,地塞米松,维生素C)、成脂肪细胞诱导液(地塞米松,甲基异丁酸黄嘌呤,牛胰岛素,吲哚美辛)及二甲基亚砜和羟基丁酸苯甲醚无血清培养基诱导剂干预细胞向成骨、脂肪、神经细胞分化,经免疫组织化学染色、PCR、免疫细胞染色方法检测成骨标志物(碱性磷酸酶、骨钙素mRNA、钙结节)、脂肪标志物(脂滴、PPARγ-2mRNA)、以及类神经标志物(尼克氏体、神经烯醇化酶、神经丝蛋白)。

In conclusion, features of morohopathology, IHC profile and ultrastructural findings reveal obvious divergence between CHP and HP and resemble MP, so we suggest that histogenesis of CHP is pericyte with myoid differentiation.

根据此研究之结果得知,无论在肿瘤发生部位、组织病理形态学、免疫组织化学染色结果以及超显微结构的特徵,CHP与HP具有明显的差异性,反而与MP之特徵较为相近,此结果可间接证实CHP之肿瘤细胞来源与MP的肿瘤细胞之来源较为相似,可能为血管周围细胞来源并具有类肌肉细胞的分化。

And (1O2) appearance. The lightening rule of the coelomocyte phagocytize yeast were curve with unimodal time by time retarting in proper condition. In different conditions, such as when pH reached 6.81, the chemiluminescence was most obviously, also when the yeast suspension concentration was 1.00×10^7 cell/ml.

在适宜条件下,体腔细胞吞噬酵母的化学发光规律随时间移动为一条有单峰值的曲线;在不同环境条件下,pH=6.81时,化学发光最明显;在外界诱导物利激下,酵母悬液浓度为1.00×10^7 cell/mL时,化学发光最明显。

The present study demonstrates that caffeic acid phenethyl ester, a major component of honeybee propolis, enhanced ATRA-induced granulocytic differentiation in HL-60, a human promyelocytic cell line. The differentiation was assessed by Wright-Giemsa stain, nitroblue tetrazolium reduction, and membrane differentiation marker CD11b.

经由实验结果发现,利用化学染剂(Wright-Giemsa stain)观察型态, CAPE也会促进低剂量 ATRA (1nM)分化的效用,而此种分化经由 NBT reduction,和透过流式细胞仪分析分化标的膜蛋白 CD11b/CD14及细胞周期测试后,证实细胞周期停滞 G1 phase与分化现象的机制有关;再藉由免疫沉降法测试,更证实细胞周期停滞将影响 CDK2的活性。

The absorption and transport of umbelliferone, osthole, columbianadin, columbianetin acetate, angelolA and angelolB are passive diffusion as the dominating process in Caco2 cell monolayer model.

为了考察独活中香豆素类化学成分在整体口服实验中能否被吸收和吸收程度,本文首先采用Caco2细胞单层模型[8]研究独活中的主要化学成分伞形花内酯、甲氧基欧芹素、二氢欧山芹素、二氢欧山芹醇乙酯、当归醇A和当归醇B的吸收和转运。6种香豆素类成分的化学结构见图1。

The effects of vacuolization on the differentiation of vascular endothelial cells were analyzed from a chemical biochemistry genetics perspective using the blastodisc cells differentiation model induced by FGF with the γ-butyrolactones derivative.

从化学遗传学的角度,利用γ-丁内酯衍生物研究了成纤维细胞生长因子(fibroblast growth factor, FGF)诱导鸡胚盘细胞向血管内皮细胞分化过程中,空泡化对血管内皮细胞分化的影响。

FDCS is a rare malignant tumor and differential diagnosis includes Langerhans cell sarcoma,interdigitating dentric cell sarcoma, malignant fibrous histocytoma, melanoma, metastatic spindle cell carcinoma and others.

正确诊断需要病理组织形态、电镜及免疫组织化学相结合,并应与朗格汉斯细胞肉瘤、指突状树突状细胞肉瘤、恶性纤维组织细胞瘤、黑色素瘤、梭形细胞癌等相鉴别。

RESULTS AND CONCLUSION: Osteoblast was fusiformed-shaped and had plentiful processes. Nucleus was orbicular-ovate and leaning to lateral side. Soma was large, and plasma was abundant. Alkaline phosphatase staining suggested that a great number of gray-black particles were observed in plasma, and some region was darkly stained.

结果与结论:成骨细胞呈梭形,多突起;细胞核呈卵圆形,偏于一侧;胞体大,胞浆丰富;碱性磷酸酶染色可见胞浆中含有大量的灰黑色颗粒,某些部位染成黑色,定量分析细胞内的碱性磷酸酶、骨钙素水平明显高于成纤维细胞;细胞免疫组织化学染色表明其主要合成Ⅰ型胶原。

No immunostaining was seen in RPE. Double staining that Kir2. 1 and glutamine synthetase, Kir2. 1 and glutamine synthetase colocalized well. Intense Kir7. 1 immunolabeling was present on the apical surface of all RPE cells and appeared to extend over the length of the apical processes. Na〓, K〓-ATPase expression varied among RPE cells, but in highly expressing cells, it co-localized with Kir7. 1. Immunoreactivity of Kir2. 1、Kir4. 1 and Kir7. 1 acomplished by ABC immunohistochemistry in monkey and human retinae got the nearly same results as bovine.

间接免疫荧光组织化学显示,Kir2.1蛋白主要分布在Müller细胞与神经元相接触的细胞膜区域;与Müller细胞的特异性标记蛋白质抗谷氨酸合成酶抗体双重标记显示其分布特性重叠,在RPE未检测Kir2.1蛋白的免疫活性存在;Kir4.1蛋白集中分布于Müller细胞的内侧突起,与GS蛋白双重标记显示其分布特性重叠,RPE未检测Kir4.1蛋白的免疫活性存在;Kir7.1主要分布于RPE游离面及其突起的全长,与特异性标记RPE突起的Ezrin蛋白完全重叠,Na〓-K〓ATP酶在不同部位的RPE表达不同,Na〓-K〓ATP高表达的RPE细胞与Kir7.1的分布特性重叠。

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