细胞化学

Methods: We divide the rats into 5 groups in random, with Morris method, detect the change of some active oxygen radicals such as super-oxide demitasse, Nitric Oxide Synthase, malondialdehyde etc. Also, we detect the change of cholinesterase, Monoamine Oxidize; using HE,PAS staining, immuno-histochemical technique, detect hippocampus CA1 cerebral pathology slices optical density value of NOS, CHE and lipofuscin of neural cell; detecting apoptosis rate and the expression of P53 , which are neuronal apoptosis related genes.

大鼠随机分为5组，采用"Morris水迷宫"法观察脑缺血性模型大鼠学习记忆功能，检测超氧化物歧化酶、一氧化氮合酶、丙二醛等自由基代谢异常的改变，检测胆碱酯酶、单胺氧化酶等老化相关酶的变化、脑组织神经细胞免疫组织化学技术、检查海马CA1区脑组织的病理切片，检测NOS、CHE光密度值；流式细胞技术：检测细胞凋亡率，及促细胞凋亡基因、P_(53)蛋白表达；病理切片采用HE染色、PAS染色及免疫组化法检查，检测对大鼠海马神经元细胞一般病理变化及脑细胞内脂褐含量。

The differentiation of the cells and the expression of Myosin Ⅶa were revealed by immunohistochemical staining.

结果脂质体介导的基因可在细胞内获得良好的表达，转染后24h，相差显微镜下观察细胞形态发生变化，免疫组织化学显示转染后细胞可以表达毛细胞特异性蛋白Myosin7a。

The results indicate that the longitudinal nerve fibers in cerebral ganglia and a few of small cells in the surface layer of cerebral ganglia present NOS positive reaction. Abundant NOS positive small cells are in the surface layer of pedal ganglia, and abundant transverse positive nerve fibers in the center of pedal ganglia. A large number of transverse positive nerve fibers are in the center of visceral ganglia; abundant positive small cells and nerve fibers are in two anterior lobes; a few of positive small cells and many encircled positive nerve fibers are in the posterior lobe; a large number of radiate positive nerve fibers are in the lateral lobes.

组织化学显示，存在NOS的部位如下：脑神经节内纵行的神经纤维和表层的少量小细胞；足神经节表层的大量小细胞，中央大量水平分布的神经纤维；脏神经节中部大量水平分布的神经纤维，前叶内大量小细胞和神经纤维，后叶内少量小细胞和许多环行神经纤维，侧叶内大量似放射状分布的神经纤维；脑足和脑脏神经索内的神经纤维。

The School of Biology and Biochemistry is primarily dedicated to teaching and research in biology and biochemistry including peptide and protein chemistry, nucleic acid radiation chemistry, parasitology, neuroscience, plant and fungal physiology, marine and coastal studies, ecology and behaviour, experimental phycology, fish genetics, molecular virology, cell genetics and microbiological transformation.

该学院主要致力于生物学与生物化学的教学与研究，其学科领域包？？？肽类和蛋白质化学、核酸放射化学、寄生虫学、神经科学、植物和真菌生理学、海洋和海岸研究、生态？？？和？？？？？为学、实验藻类学、细胞遗传学与微生物转化。

The results showed that there existed variation among eucalypt families in wood physical-mechanical properties and wood chemistry. There were significant differences among E.pellita families、E.grandis families and E.urophylla譋.grandis clones in wood colors as well as between Strains and within trees.The variation of the surface wetability of wood between E.grandis families and E.urophylla譋.grandis clones were significant.Even in the same families level or clones level,there still existed the same regulation. The degree of collapse of eucalypt had a large relation in the amount of tylosis in vessel and vestured pits on the cell wall of vessel. The more rich tylosis and vestured pits in vessels,the more probability to occur collapse for eucalypt.The biggest moment collapse was probably the critical point of the drying degrade.During the fomulation of drying technology,it is necessary to adopt moderate condition to make the vessels which had formed the biggest moment collapse at the biggest moment collapse resume,especially to prevent the permanent set.At the normal temperature level,the main factors which influenced residual collapse were the contents of ray parenchyma and axial parenchyma .Nearly all the cells joined the course of forming the moment collapse and biggest moment collapse.Interval drying was fit for eucalypt plantation which was easier to make collapse.

研究表明：桉树不同家系间木材物理力学性质和化学组成存在差异；粗皮桉家系间、巨桉家系间、尾巨桉无性系间材色存在显著差异，株间、株内木材材色存在变异；桉树木材表面润湿性在巨桉家系间、尾巨桉无性系间存在着显著差异，在同一家系或同一无性系中，株间和株内也存在着相同的变化规律；桉材皱缩程度与其导管中侵填体的多少和导管壁上纹孔具有的附物多少密切相关，侵填体含物越丰富，纹孔附物越多，桉材越容易皱缩；最大瞬间皱缩是能产生更大干燥降等的临界点，在制定干燥工艺时，必须在最大瞬间皱缩发生时，采用温和条件，使已产生最大瞬间皱缩的细胞尽可能多的恢复，尤其不能使其产生永久变定；在常温条件下，影响残余皱缩的主要因子是射线薄壁细胞和轴向薄壁细胞含量；而对于瞬间皱缩和最大瞬间皱缩，几乎所有细胞都参与它们的形成过程；对于易皱缩的桉树木材，间歇干燥是最有前途的干燥方法。

Cardiac hypertrophy is one of adaptive responses of the heart to a variety of pathological stimuli, including hypertension, myocardial infarction, congenital heart disease, heart valvula disease, and perturbations in sarcomeric function due to altered expression or mutations of contractile proteins. Increase in wall tension and hormonal stimuli are thought to directly activate various membrane-bound receptors and signal transduction cascade within cardiomyocytes resulting in the activation of immediate early genes and responsive genes such as c-jun, c-fos, c-myc, egr-1, ANF, BNP. Then the expression of construction proteins such as MLC-1, skeletal α-actin was unregulated.

中文题名hhlim基因的表达调节及其与心肌细胞肥大发生之间的关系副题名外文题名 hhlim gene expression regulation and its role in development of cardiomyocyte hypertrophy 论文作者郑斌导师温进坤韩梅学科专业生物化学与分子生物学研究领域\研究方向学位级别博士学位授予单位河北医科大学学位授予日期2003 论文页码总数106页关键词心肌细胞肥大基因表达心肌肥厚 hhlim基因馆藏号BSLW /2003 /R542.2 /33 心肌细胞肥大是高血压、心脏瓣膜病、急性心肌梗塞及先天性心脏病等临床常见疾病的一种并发症，是心肌细胞对多种病理刺激的一种适应性反应。

Objective To observe the pathological changes and investigate the hepatocyte apoptosis relating with CYP2E1 in alcoholic liver diseaseof rat. Method the Model of Alcoholic liver Disease: Rats were randomly divided into model group(n=37)and control group(n=33). The dose of 40％ethanol was administerd at 8g/kg body weight by garage twice daily for 8 weeks in model group, and control group was received isovolume saline by gavage. End of 8th week, the activities of serum ALT and AST were detect by autobiochemistry instrument. The pathological changes of liver was observed under light microscope after HE staining, hepatocyte apoptosis was detected by the TUNEL method.

目的： 研究酒精性肝病细胞凋亡程度与细胞色素P4502E1表达的关系方法：用酒精灌胃法制备大鼠酒精性肝病模型，将70只wistar大鼠随机分为正常对照组(33只)和酒精灌胃组(37只)，连续灌胃8周后病理切片观察细胞凋亡程度，干化学法测定ALT、AST的含量变化，用PCR法测定细胞色素P450 2E1的表达变化。

Results NGF and its mRNA were localized in chondrocytes in the sclera, especially in the binucleate cell. In NGF and its mRNA respectively, the amount of the positive cells in the occluded eyes was significantly higher than that of the control eyes (P.05). In NGF F(subscript DG=20.556, P.001 or in its mRNA F(subscript DG=25.520, P.001, the amount of the positive cells among the occluded groups or the control groups was significantly decreased along with the time.

结果 NGF蛋白及其mRNA均表达於巩膜的软骨细胞中尤其在双核细胞中；两者在各时间段遮盖眼巩膜中阳性细胞数均明显高於对照组(P.05)；免疫组织化学F(下标 DG=20.556 P.01和原位杂交F(下标 DG=25.520 P.01在对照组各组间阳性细胞的表达数目随时间变化明显下降。

Using cytohischemical staining methods, with the results of comparison the dynamics of proteins and polysaccharides in the anthe wall cells and colule cells of different developmental phases in female and male flowers, the anormogenesis of anther tapetum in the inflecting phase of from bisexual flower to unisexual flower was observed. In microspore developed phase, the tapetum functions of preserving and transportingmutritive material for microspore development and of secreting callosal enzyme for decompositing callosal cell lost no normal guadrant formed, of being abnormal meiose of pollen mother cells, and then, the stamen aborted selectively in female flowers and pistil in male flower.

利用细胞组织化学染色技术，对雌、雄花雄蕊花药壁细胞及花药内细胞发育过程中多糖及蛋白质动态进行了比较，实验中观察到雌花在从两性至单性花转变时期雄蕊绒毡层在整个发育过程中表现异常，在小孢子发育过程中未能起到贮藏、转运营养物质供小孢子发育及适时分泌胼胝质酶溶解胼胝质壁的功能，并且花粉母细胞减数分裂异常而未形成四分体结构，进而导致雌花雄蕊选择性败育，而雄花中雌蕊组织也发生了选择性败育过程。

The mechanism of Si Junzi Tang and panax sapogenol were studied on the effect of gastrointestinal cells protection by applying advanced technology of histochemistry, Holt′s cytodieresis mircocirculation, radio-immunity and subcellular ultrastructure.

进而应用酶组织化学、Holt细胞分裂测定、微循环测定、放射免疫测定、亚细胞超微结构观察等先进技术，研究四君子汤、人参皂甙抗胃肠细胞损伤、保护胃肠细胞的作用机理。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。