细胞化学

More thicker epiphyseal plate were found in the left femurs than the right femurs of the group fixed with plates at 3 and 6 month after operation. In the group fixed with plates, Safranine O staining showed epiphyseal plates ot the left femurs had more fuscous staining than the right femurs at 3 and 6 month after operation. Electron microscopy also found that the cells of the epiphyseal plates of left femurs were more eugenic than the right femurs in the group fixed with plates at 3 and 6 month after operation.

光镜学实验结果示滑动钢板固定的左侧股骨骨骺板总厚度及肥大细胞计数均明显大于普通组；组织化学观察示滑动钢板固定的左侧股骨骺板于术后3个月及6个月Safranine O组织化学染色明显深于普通组；电镜观察示滑动钢板固定的左侧股骨骨骺于术后3个月及6个月软骨增殖区软骨细胞分裂及生长较普通钢板固定的右侧活跃。

Experts, the expansion of the chemical agent called motilin cells, hormones are chemical substances, commonly used fruits and vegetables have a kiwi, watermelon, strawberries, cherries, tomatoes, and some vegetable farmers in the cucumber, summer squash and other vegetables used in this way to make fruits and vegetables-cell non-normal enlargement, head of fruits and vegetables grown up than the normal 1-2 times as large, the shape become more exotic, such as tomatoes, long tip, strawberries were piriform, cucumber apex hypertrophy with pedicle sharp contrast and so on.

专家介绍，膨大剂的化学名称叫细胞集动素，属于激素类化学物质，常用的果蔬有猕猴桃、西瓜、草莓、樱桃、西红柿等，有些菜农在黄瓜、西葫芦等蔬菜上使用，以此方法使果蔬细胞非正常膨大，个头比正常长大的果蔬大1—2倍，形状变得比较奇特，如西红柿长尖、草莓呈梨状，黄瓜尖部肥大与蒂部形成鲜明对比等。

Methods RGD peptide were through direct covalent linkage attached on micro-channel surface with 1-ethyl-3- 3-dimethylaminopropyl carbodiimide hydrochloride and N-hydroxysuccinimide to A549 cells.

方法采用化学共价连接的方法固定RGD多肽，建立由流体控制系统、免疫反应系统构成的细胞识别芯片系统，用于细胞的筛选/捕获；选用不同密度的细胞进行进样试验，考察不同细胞密度对通道内细胞数量的影响，并讨论不同的孵育时间对细胞捕获的影响。

Methods RGD peptide were through direct covalent linkage attached on micro-channel surface with 1-ethyl-3- 1 3-dimethylaminopropyl carbodiimide hydrochloride and N- hydroxysuccinimide to A549 cells.

采用化学共价连接的方法固定RGD多肽，建立南流体控制系统、免疫反应系统构成的细胞识别芯片系统，用于细胞的筛选／捕获；选用不同密度的细胞进行进样试验，考察不同细胞密度对通道内细胞数量的影响，并讨论不同的孵育时间对细胞捕获的影响。

However, the molecular mechanisms underlying premature aging up 8-MOP/UVA are as yet unknown. In our studies, dermis of the non-lesion back skin of vulgar psoriatic patients who had been treated by PUVA and the cultured foetus dermic fibroblasts were selected to be observed.

本课题以PUVA治疗寻常型银屑病患者背部非皮损区真皮及培养的胎儿真皮成纤维细胞为对象，采用HE染色、Verhoeff染色、电镜、酶组织化学、免疫组织化学、原位杂交、流式细胞计数仪、三引物PCR、PCR-SSCP技术及PCR产物直接测序等技术和方法，研究了8-MOP/UVA对皮肤光老化形成的影响及芳维A酸乙酯的拮抗作用。

Methods The NGF, BDNF, and NT3 genes of rats were cloned, the eukaryote expression vectors were established, the three kind of recombinant vectors were used to transfect astrocytes, the positive cloned cells were cultured dilatedly after G418 sifting; using supernates of culture liquid of astrocytes modified by gene to culture PC12 or TrkB-PC12, the expression and its level of gene target cells aimed genes were measured by Western blotting or immunohistochemical method.

克隆大鼠NGF、BDNF和NT3基因，构建真核表达载体；3种重组载体分别转染星形胶质细胞，G418筛选后获得阳性克隆细胞进行扩大培养；取基因修饰星形胶质细胞培养液上清培养PC12细胞或TrkB-PC12细胞；用Western blotting杂交或免疫组织化学方法检测基因靶细胞目的基因的表达及其水平。

In order to distinguish the type of GFP positive cells in glomeruli further, we also carried out fluorescence histochemistry double staining, the results of it were: the GFP positive cells in recipients glomeruli in mice of the four, five, six groups were blood cells in glomeruli capillary loop, but not the glomeruli resident cells, for example, endothelial cells, epithelial cells or intercapillary cells.

为了进一步鉴别受体鼠肾小球内GFP阳性细胞的种类，我们进行了免疫荧光组织化学双染色，结果显示：④、⑤、⑥组受体鼠肾小球内的GFP阳性细胞可能是肾小球毛细血管袢内的血细胞而非肾小球的固有细胞，即内皮细胞、上皮细胞或系膜细胞。

After HRP application, HRP labelled cells were distributed in the nucleus of trapezoid body , lateroventral periolivary nucleus , retrotrapezoid nucleus , lateral paragigantocellular nucleus and lateral reticular nucleus . The findings demonstrated the existence of neural structural relations between the chemosensitve areas and the Tz, LVPO, NRT, LPGi and LRt, and revealed that these nuclei might participate in the mechanism of central chemical regulation on respiration.

观察到用浸有HRP的滤纸局部敷贴于延髓腹外侧表面化学感受区后，在延髓斜方体核、外周橄榄腹外侧核、斜方体后核、巨细胞旁外侧核和外侧网状核等核团找到HRP标记的细胞，表明上述延髓内部核团与腹外侧表面化学感受区之间存在神经结构联系，提示延髓腹外侧表面化学感受区对H〓等物质的化学感受机制可能与上述核团神经元伸向该区的突起有关。

There were 8 patients in the macromastia group and 5 patients in the control group. The result didn't show significant difference between the two groups. There is no difference between the two groups in mammary epithelial cell size measurement and Akt-1/2/3 immunohistochemistry stain. The levels of Ki-67, phospho-Akt-1/2/3, and STAT5a of juvenile macromastic patients are higher than those of the patients of the control group, and the results are statistically significant.

三年内共搜集到八位巨乳症病患及五位对照组，两组以Wilcoxan rank sum test比较，乳腺上皮细胞的尺寸及Akt-1/2/3免疫组织化学染色方面在统计学上的没有显著的差异，而乳腺上皮细胞之Ki-67、phospho-Akt-1/2/3、及STAT5a免疫组织化学染色方面，则均为青春期巨乳症患者组较高，统计学上均有显著性的差异。

Compared with those in control group,the number of positive immunoreactivity cell in RSR and intestine increased and its intensity of immunoreactivity enhanced obviously after rabbit was infected with Pasteurella multocida.

免疫组织化学观察发现，产抗菌肽细胞在兔圆小囊的黏膜上皮、圆顶上皮以及淋巴组织的滤泡生发中心、圆顶区和帽区均有分布；在兔感染多杀性巴氏杆菌后，圆小囊及其他肠道组织中的抗菌肽免疫组织化学阳性细胞数量明显增多，阳性反应增强。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。