细胞化学

RESULTS: A great deal of neurospheres were formed in the presence of the extracts of afterbrain and cerebellum, which were positive for characteristic protein of NSC showed by immunocytochemical staining.

结果： 中脑和小脑的提取液加入后有大量神经球生成，神经干细胞的特异性蛋白nestin免疫细胞化学染色呈阳性。

2 The result of electron microscope cytochemistry stain: The positive production of ACPase was present black lead phosphate deposit with high electron-dense under light microscope, located in cytoplasm or apophysis mainly. Lysosome was located in cytoplasm in the earlier of differentiation, then was present in apophysis with it grew.

4.2 电镜酶细胞化学染色结果：电镜下ACPase阳性产物为电子致密度高的黑色磷酸铅沉淀，主要分布于神经元的胞浆中或突起内，神经元分化的初期，溶酶体集中分布于胞质内，随突起的长出，可见在突起内出现。

Abstract］ Objective To investigated the cellular distribution and quantitatively of CEA in cerebrospinal fluid meningeal carcinomatosis cells by immunofluorescence staining and laser scanning confocal microscopy, explore the diagnostic value of the examination in the patients with meningeal carcinomatosis.

目的 联合应用免疫荧光细胞化学技术和激光扫描共聚焦显微镜技术检测脑脊液中脑膜癌细胞CEA的表达部位并定量测定其荧光含量，探讨该技术在脑膜癌病诊断中的价值。

Methods:Twenty one cerebrospinal fluid specimens from 13 patients with meningeal carcinomatosis and 7 specimens from 5 non meningeal carcinomatosis patients were tested with routine cytologic examination and CEA immunocytochemistry examination.

结果 ：常规脑脊液细胞学检查阳性率 4 2 。86 %(9/ 2 1 )，CEA免疫细胞化学检查阳性率 80 。95 %(1 7/ 2 1 )，两者相差显著(P 。0 5 )；其中对首次腰穿获得的脑脊液标本检查，两种方法的阳性率分别为 30 。77%(4 / 1 3)和 76 。92 %(1 0 / 1 3)，两者相差也显著(P 。0 5 )；非脑膜癌病患者的脑脊液检查结果均为阴性。

Ultrastructural localization of ATPase activity in cells of leaf Chlorenchyma of Aloe vera L.

利用电镜―细胞化学技术研究了盐胁迫下芦荟（ Aloe vera L。

METHODS By using immunohistochemical method,the expressions of PKCβⅠ and PKCγwere examined in ginea pig macula utriculus,macula sacculus and crista ampullaris.Theγ-32 P was used as a tracer to measure the activity of PKC in vestibular terminal organs.

采用免疫细胞化学法观察椭圆囊斑、球囊斑和壶腹嵴PKCβⅠ，Ⅱ，γ亚型的表达；应用γ-32 P示踪检测前庭终器PKC活性的变化。

Real-time PCR and immunofluorescein cytochemistry were performed to analyze the expression of RAGE after 2 days' stimulation of IL-1β.

IL-1β刺激2天后使用实时PCR和免疫荧光细胞化学分析RAGE受体的表达。

Methods: The distribution of CMPase in the retina of 20-week-old WKY and SHR were studied by electron microscopic cytochemistry.

应用电镜酶细胞化学方法对 20 周龄正常京都种大鼠和同周龄的自发性高血压大鼠视网膜组织的胞嘧啶单核苷酸酶活性部位进行定位观察。

Third, the prepared McAbs were identified by cytochemistry, specificity and Western-blot.

然后用细胞化学、特异性、Western-blot等方法鉴定制备抗体的免疫反应及特异性。

Methods: Using biotin labelled ECL, UEA and PHA-L as probes, we examined the distribution and dynamic changes of their receptors with affinity cytochemistry and image analysis.

以生物素标记的凝集素ECL、UEA和PHA-L为探针，应用亲和细胞化学和图像分析的方法，检测上述3种凝集素相应受体的分布状况和变化规律。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。