细胞化学

The expression of actin was tested by immunocytochemistry.

免疫细胞化学检测肌动蛋白的表达。

Methods: Human luteinized granulosa cells were collected from patients undergoing in-vitro fertilization-embryo transfer.

收集体外受精－胚胎移植过程中的人黄素化颗粒细胞进行体外培养；应用RT-PCR法和免疫细胞化学法分别检测HB-EGF的mRNA和蛋白表达；应用实时RT-PCR法检测HB-EGF mRNA量的变化。

HSP70 existed in cytoplasm in normal condition, and predominantly translocated into nucleus after heat shock.

免疫细胞化学技术发现：L-02细胞在正常培养时，HSP70表达很低，且主要分布于胞浆；应激后表达明显增加且大部分分布于细胞核。

Objective To study the effects of berbamine and batiolun on LDH metabolizability in the process of increasing leukocyte.Methods Enzyme biochemical techniques is used to study the effect of berbamine and batiolˉun in the process of inceasing leukocyte and also to study the effect of LDH metabolizability.

目的 研究小檗胺和鲨肝醇升高白细胞作用及对细胞内LDH的代谢的影响方法采用酶细胞化学方法，对两药物在治疗由免疫抑制剂环磷酰胺所致的白细胞减少症时，对乳酸脱氢酶活性的影响进行了比较观察。

RESULTS After treatment with CDDP at15mg L-1 ,there were apoptotic peaks in cell cycle analysis on FCM for48h and expression of MAPKs and transcripton factors in cell plasm and/or nucleus for72h.

结果 15mg L-1 顺铂作用肝癌SMMC-7721培养细胞48h可观察到明显的凋亡峰，72h免疫细胞化学染色发现胞质或胞核中MAPKs（JIN/SAPKs，p38MAPK，ERK）及转录因子均有明显表达。

Methods The chemical hypoxia was induced by incubation of U251 cells with cobalt chloride.

以氯化钴模拟缺氧，采用RT-PCR、免疫细胞化学方法分别检测缺氧诱导因子-1α和血管内皮生长因子mRNA和蛋白的表达，鸡胚绒毛尿囊膜模型检测缺氧时胶质瘤细胞血管生成情况。

Mechanical strains also regulated the protein and mRNA expression of several differentiation markers, as well as the activation of extracellular signal-regulated kinases, p38 MAP kinase and protein kinase B in a frequency-dependent manner. Furthermore, the inhibition of p38 pathway could block the strain-frequency induced the phenotype modulation of VSMCs, neither ERKs nor Akt. Frequency of mechanical strain, not conditioned medium, regulated the phenotype of VSMCs in a frequency-dependent manner. Rho-GDI alpha was suppressed by the mechanical strain at 1Hz.

采用免疫细胞化学法检测VSMCs形态和排列的变化；RT-PCR和Western blotting检测表型标志分子α-肌动蛋白、肌球蛋白重链(SM1/2)、肌动蛋白相关蛋白SM22α和调宁蛋白(h1-calponin)的mRNA和蛋白水平的变化；抑制剂或RNA干扰阻断可能的信号调节分子的活性或表达，包括p38、细胞外信号调节激酶1/(2extracellular signal-regulated kinases， ERK1/2)、蛋白激酶B和Rho-鸟苷酸解离抑制因子(Rho-guanine nucleotide dissociation inhibitor， Rho-GDI alpha)，研究了不同频率张应变对VSMCs表型转化的影响及其调节机制。

Because of the low efficiency, only those detection methods that dont require too much cells, such as immunocytochemistry and RT-PCR, can be adopted in the traditional way, which limites the application of this differentiation model in science research.

传统ATRA法诱导分化的效率较低，只能采用免疫细胞化学或PCR等细胞需求量较小的方法进行相关检测，这样，就大大限制了这一分化模型在科研中的应用。

Methods The guts of embryonic mice removed and dissociated were plated into serum-free DMEM/F12 medium. The mitogen-free DMEM/F12 medium supplementd with 10% fetal bovine serum was used to induce differentiation of GNCSCs. Neurospheres and their derivations were determined with immunocytochemical and immunofluorescent staning.

分离胚鼠肠管，消化后接种于添加N2、B27和碱性成纤维细胞生长因子的DMEM/F12培养基，贴壁培养；连续传代后用血清促进GNCSCs分化；最后用免疫细胞化学方法染色鉴定。

Methods The guts of embryonic mice removed and dissociated were plated into serumfree DMEM/F12 medium. The mitogenfree DMEM/F12 medium supplementd with 10% fetal bovine serum was used to induce differentiation of GNCSCs. Neurospheres and their derivations were determined with immunocytochemical and immunofluorescent staning.

分离胚鼠肠管，消化后接种于添加N2、B27和碱性成纤维细胞生长因子的DMEM/F12培养基，贴壁培养；连续传代后用血清促进GNCSCs分化；最后用免疫细胞化学方法染色鉴定。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。