细胞化学

Results The island stem cells cultured extrinsically were differentiated into epithelioid cells after having been induced by ITS for 3 days.

细胞特异性成分胰高糖素等的免疫细胞化学染色鉴定。

At 5 days, immunocytochemical staining demonstrated positive reactions for glial fibrillary acidic protein and neuron specific enolase.

神经干细胞球诱导5 d，免疫细胞化学染色后可见胶质纤维酸性蛋白及神经元特异性烯醇酶呈阳性表达的细胞。

The plasmalemma of cells within meristematic regions was observed to possess invaginations in cultured callus of Stevia rebaudiana under differentiation.

对在分化条件下的甜菊愈伤组织分生区域细胞的质膜内陷进行了超微结构和酸性磷酸酶细胞化学研究。

Some researchers have found that SP could affect other neurons in enteric nervous system . However, little is known about the mechanism of SP on the myenteric cholinergic neurons.

本研究采用全细胞膜片钳技术和免疫细胞化学技术探讨了SP对豚鼠回肠肌间丛胆碱能神经元电活动的影响，为揭示肽类递质对胆碱能神经元的调节机制提供依据。

Methods The neuroepithelial stem cells were isolated and cultured by microdissection, mechanical blowing and serum-free suspension culture.

方法采用显微解剖、机械吹打、无血清悬浮培养方法分离培养神经上皮干细胞，采用巢蛋白免疫细胞化学染色技术检测神经上皮干细胞，用NSE和GFAP免疫组化染色检测并计数神经细胞和神经胶质细胞。

After 3 days treatment with RA, the cells began to show phenotypical changes, assuming a neuronal-like morphology with contemporaneous formation of dendritic-like structures.

在诱导的d1、3、7，用相差显微镜观察细胞形态的变化；用免疫细胞化学染色、RT—PCR等方法检测p27^Kip1、p21Cip1及其相关的cdk2、cyclinE的变化。

BrdU-immunoreactive cells were confined to the subgranular zone in the dentate gyrus, and displayed oblong or irregularly shaped nuclei when three different doses of doses of BrdU were given to adult mature rats.

正常成年大鼠腹腔注射BrdU 1Omg/kg、BrdU 50mg/kg、BrdU 100mg/kg后，免疫细胞化学ABC法显示BrdU阳性细胞主要分布在齿状回亚颗粒增生带，表现为椭圆形或不规则形状的细胞核，有丛集现象。

We observed the effect of chronic hypoxia on intracelular levels of CGRP in pulmonary endocrine cells of SD rats by means of immunocytochemistry and supraoptimal dilution method.

我们用免疫细胞化学方法及超稀释法观察了慢性缺氧对SD大鼠肺中神经内分泌细胞内降钙素基因相关肽的含量的影响。

The changes in the weight, exponential, structure of the tissue, cell apoptosis in testis and epididymis and the changes in the sperm tectology in epididymis were measured on the day 8, 13, 21 and 35 of the treatment,respectively,by the method of morphological examination.

分别在热应激持续到8d、13d、21d和35d时，应用形态学观察法，研究受试鼠睾丸和附睾重量及指数、组织结构、细胞凋亡和附睾精子形态学的变化；应用免疫荧光细胞化学和蛋白质印迹分析的方法，研究睾丸和附睾组织中热休克蛋白70(HSP70)的表达；应用比色法检测睾丸和附睾组织内活性氧和丙二醛、抗氧化物酶(SOD、GSH-Px、CAT)的活力或浓度的变化；应用交配试验，检测雄鼠受精能力及其致孕雌鼠的产仔数的变化，用以揭示热应激对雄鼠生殖机能的影响。

It was found that after differentiation the cells expressed the endocrine markers insulin 1, glucagons, somatostatin by RT-PCR, however, these endocrine markers cannot be found in uninduced cells.

通过免疫细胞化学染色可以检测到在ICCs中存在有胰岛素、胰高血糖素、生长抑素和pdx-1阳性的细胞，通过RT-PCR可以在mRNA水平检测到胰岛素、胰高血糖素、生长抑素、pdx-1、ABCG2、葡萄糖转运蛋白等的表达。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。