细胞化学

Methods Human pancreatic carcinoma cell lines (n=4) were studied by using cell culture, immunocytochemistry and Northern blotting analysis.

采用细胞培养、免疫细胞化学和Northern印迹分析方法。

The distribution of SYP and the ultrastructures of SYP immunoreactive cells and immunoreactive products in intercellular space of the sacculus rotundus of rabbits were investigated using immunohistochemistry and immune electron cytochemistry methods.

采用免疫组化和免疫电镜细胞化学的方法，对家兔圆小囊组织中的SYP阳性细胞和胞间阳性反应物的光镜和电镜超微结构特点进行了研究。

Immunofluorescence was used to analyze the expression of the SMA and identify the cells.

通过免疫细胞化学技术检测血管平滑肌肌动蛋白的表达来鉴定细胞性质。

The electron-dense lead phosphate deposits, an indication of ATPase activity, mainly distributed on the plasma membrane and tonoplast in the mesophyll cells of palisade and spongy tissue, in which there is no obvious differences of ATPase distribution and activities.

以及两者在-5℃下处理1d后的叶肉细胞进行了ATPase的超微细胞化学定位的比较观察，并对冷驯化可提高植物抗寒力及ATPase与植物抗寒性关系进行了探讨。

Immunocytochemistry localization shows SBP117 is mainly located at the bulliform cell in the leaf. Tissue printing study showed that SBPl 17 is mainly located at the epidermis of roots, shoots and leaves as well as in the vascular bundle of the rice roots and leaves. The distribution of SBPl 17 is coincided with the sites of Si accumulated in rice plants.

免疫细胞化学检测SBP117主要分布在水稻叶片的泡状细胞中；组织印迹法显示SBP117主要分布在水稻根茎叶的外表皮中，在根和叶的维管组织中也有分布，这与硅在水稻体内的分布是一致的，说明此蛋白参与控制硅在水稻体内的沉积。

The activities of succinate dehydrogenase in hepatocytes, alkaline phosphatase and thiamine pyrophosphatase on the wall of bile canaliculi became obviously less.Both the activities and localizations of TPPase had changed. Some TPPase from the damaged lysosome-like vesicles and Golgi saccules of hepatocytes discharged into the cytoplasm. TPPase reaction product in some bile canaliculi decreased.

酶细胞化学技术显示肝细胞线粒体琥珀酸脱氢酶和毛细胆管壁上的碱性磷酸酶活性明显减弱或消失，焦磷酸硫胺素酶的活性及定位也发生了改变， TPPase从损伤的高尔基复合体及溶酶体样小泡漏出进入胞浆，部分毛细胆管壁上的TPPase反应物减少。

The immunocytochemistry position of cell cycle proteins in hepatocellular carcinoma cytogeny HCC????

杨连君，王文亮，司晓辉。细胞周期相关蛋白在肝癌细胞系HCC？？？？9204表达的免疫细胞化学定位［J］。

Under fluorescence microscope, cytolymph could emit red fluorescence with Rhodamine, and cellular nucleus could emit blue fluorescence with DAPI, accompanying myofilament structure.

免疫细胞化学检测：荧光显微镜下，罗丹明染色细胞浆呈红色荧光，并可见肌丝结构；DAPI染色细胞核呈蓝色荧光。

The cell from hippocampus, striatum, ependyma, thalamus opticus, olfactory bulb, cortex, brain stem, cerebellum, and spinal cord in human fetal brain were studied using HE staining and immunocytochemistry.

应用HE染色和免疫细胞化学方法观察人胎脑室管膜下区、海马、纹状体、丘脑、嗅球、皮层、脑干、小脑、脊髓的Nestin阳性细胞。

Using electronic microscopy and cytochemical methods, we studied the distribution changes of the cell ergastic substance and development of secondary vascular tissues. Development of secondary vascular tissue took on an inherent rhythm, showing alternation of dormant and differentiated stages in periodicity of one year, and the formation of secondary phloem and xylem was alternate.

利用电镜技术及细胞化学方法，研究了美洲黑杨次生维管组织的发育以及细胞内含物分布的变化，发现次生维管组织的发育具有内在节律性，在年周期中表现为分化期和休眠期交替出现；次生韧皮部与次生木质部交替分化形成。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。