细胞化学

The results show that firstly, ApoE and LDL receptors can be transported bidirectionally (i.e. retro-and anterogr-adely) in the axoplasm along axons. Secondly, ApoE derived from regenerating fillers can be transported re t rogradely to the cell bodies.

本文用免疫细胞化学法研究正常和再生的大白鼠外周神经中脱辅基脂蛋白E和低密度脂蛋白受体的运输特性，发现在正常的外周神经中，ApoE及LDL受体在轴浆中均可沿轴突正行和逆行运输；再生的神经可产生ApoE，ApoE可逆行向细胞体转运。

The expressions of HO-1 and HO-2 mRNA were detected using reverse transcription polymerase chain and the relative amount of CO released into the media was quantitated as carboxyhemoglobin by enzyme linked immunosorbent assay.

通过逆转录聚合酶链式反应和免疫细胞化学法检测HO-1、HO-2 mRNA和蛋白的表达，酶联免疫法检测培养上清液中碳氧血红蛋白含量，四唑氮比色法检测细胞增殖。

Methods Expression of LEA and CEA in colorectal carcinoma cells was detected with immunocytochemistry and flow cytometry .

采用流式细胞仪和免疫细胞化学染色检测大肠癌细胞系LEA和CEA的表达。

The result of immuno-electron cytochemistry showed the number of positive reaction cells of gastrin increased significantly.

胃泌素免疫电镜细胞化学观察结果显示阳性细胞数量明显升高。

The aim of this study was to establish the culture system of isolation and cultivation of the neural stem cells from the embryonic rat brain and spinal cord. The methods of microscopic dissection, cell culture and immunofluorescence cytochemistry were used.

研究采用显微解剖、无血清细胞培养和免疫荧光细胞化学染色等实验技术，成功地建立了胚胎大鼠脑和脊髓神经干细胞的分离和培养方法。

The aim of this study was to establish the culture system of isolation and cultiation of the neural stem cells from the embryonic rat brain and spinal cord. The methods of microscopic dissection, cell culture and immunofluorescence cytochemistry were used.

研究采用显微解剖、无血清细胞培养和免疫荧光细胞化学染色等实验技术，成功地建立了胚胎大鼠脑和脊髓神经干细胞的分离和培养方法。

Immunocytochemical studies showed that the transduced muORs were expressed in all types of DRG neurons.

免疫细胞化学研究表明转导的 muORs在背根神经节神经元的各种类型的细胞中都能表达。

Methods:Via a cannula iserted into adominal aorta, Gey's balanced salt solution containing 0.025% collagenase P was perfused into rat normal pancreas. After digestion with collagenase P and protease, Nycodenz density gradient centrifugation was performed to isolate PSC. PSC was identified by its morphology, cytoplasmic lipid droplets and immunocytochemical staining for desmin, GFAP and α-SMA. Results:The production,viability and purity of isolated PSC were（15.3 ± 4.6）× 103／g body weight,（95.0 ± 3.5）%, and ﹥80％ respectively.

大鼠腹主动脉插管灌注后，经胶原酶消化、Nycodenz密度梯度离心，分离得到PSC；通过观察细胞形态、胞质内脂滴和免疫细胞化学方法检测结蛋白、神经胶质酸纤维蛋白（glial fibrillary acidic protein，GFAP）、α平滑肌肌动素（α-smooth muscle actin，α-SMA）的表达来鉴定PSC。

Before transfection, the cells had a significant heteromorphism, with the fea- tures of large cell body, little cytoplasm, obvious karyokinesis.

以重组细胞系作为研究模型，通过免疫细胞化学及Westemblot确认Caveolin—1蛋白在被转染细胞中的稳定表达。

At the leptotene stage the enzyme was mainly present in the cisternae of smooth endoplasmic reticulum and their derived vesicles, but absent in the Golgi body and Golgi vesicles.

花粉母细胞减数分裂前期Ⅰ的活性进行了电镜细胞化学定位，以研究其在次生胞间连丝和胞质通道形成中的作用。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。