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细胞化学

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Cellulase activity in adventitious roots of maize during aerenchyma formation induced by flooding was located through Cellulase activity in adventitious roots of maize during aerenchyma formation induced by flooding was located through the reaction of Benedict's reagent with reducing sugars liberated by hydrolysis of carboxymethylcellulose.

采用本氏试剂与羟甲基纤维素水解还原糖反应的细胞化学方法,对玉米不定根淹水诱导形成通气组织过程中,逐步解体的皮层细胞内纤维素酶活性进行了定位观察。

Cellulase activity in adventitious roots of maize during aerenchyma formation induced by flooding was located through the reaction of Benedict's reagent with reducing sugars liberated by hydrolysis of carboxymethylcellulose.

采用本氏试剂与羟甲基纤维素水解还原糖反应的细胞化学方法,对玉米不定根淹水诱导形成通气组织过程中,逐步解体的皮层细胞内纤维素酶活性进行了定位观察。

METHODS: The extracts of forebrain, midbrain,afterbrain and cerebellum of neonatal mice were prepared, and the NSCs of newborn mice were cultured in vitro. Neurospheres were observed, immunocytochemical staining of characteristic protein, nestin, and MTT assay were performed to identify NSCs and their proliferative properties.

制备新生鼠的前脑、中脑、后脑及小脑组织提取液,体外培养新生鼠神经干细胞,观察神经球的生长情况,采用MTT细胞活性检测法和特异性蛋白质免疫细胞化学染色鉴定神经干细胞的增殖能力。

Methodological contributions as well as papers in the fields of applied histo- and cytochemistry e.g.

该杂志发表整个组织化学和细胞化学领域内的综合和分析评论文章。

This is the Progress in Histochemistry and Cytochemistry publishes comprehensive and analytical reviews within the entire field of histochemistry and cytochemistry.

这是《组织化学和细胞化学的进展》的网页。

The expression pattern of PKC and morphology were investigated during inflammatory pain using immunocytochemistry and histochemistry.

应用组织化学和免疫细胞化学方法观察炎性痛过程中海马神经元形态学变化和PKC活性的改变。

In this study, the expression and function of FGF-13 in developmental follicles and skin during embryonic development,normal vibrissa follilce stem cells and wound follicles in vitro were obserbed,so as to illuminate the possible role of FGF-13 in the skin follicles during embryonic development and damage healing and provide the experimental base for further studying the characteristic and mechanism of the role of FGF-13 in skin.

采用免疫组织化学(Immunohistochemistry,IHC)、免疫细胞化学(Immunocytochemistry, ICC)和原位杂交(in situ hybridization,ISH)技术及体外大鼠触须毛囊的器官培养,研究了三种不同的生物学过程中FGF-13在皮肤毛囊中的表达及作用。

Then, the proliferative ability of OPCs in vitro was also investigated with the MTT assay.4. The contusive injury of spinal cord in a rat model was produced by the means of the weight-drop impact of Allen's method. The experimental animals were randomly divided into 4 groups, i.e. OPCs transplant group, transplant control group, plain injury group and sham operation group.

OPCs在体外化学条件培养基中继续生长,通过相差显微镜连续观察和免疫细胞化学技术检测研究分析体外条件下OPCs的定向分化规律和分化成熟情况,并采用MTT检测法研究分析OPCs在体外条件下的分裂增殖能力。4。

MAIN OUTCOME MEASURES: Cell growth and morphological changes were observed with an inverted phase contrast microscope.

主要观察指标:倒置相差显微镜下观察细胞生长情况和形态学变化,免疫细胞化学染色检测CD34、Ⅷ因子的表达,透射电镜观察细胞超微结构。

Methods Lymphatic endothelial cells were isolated from pig thoracic duct, primary cultures and subculture were finished by collagenase digestion method, immunocytochemstry was used in identification of their biological characterization.

用胶原酶灌注消化的方法,从猪胸导管中分离出淋巴管内皮细胞进行原代及传代培养,应用免疫细胞化学法对培养的细胞进行鉴定。

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