细胞化学

METHODS: Osteoporosis model was prepared with injection of dexamethasonc. Antagonism osteoporosis acupoint paste on acupoints and non-neridian places was administrated to compare with oral recipe for kidney tonification in the treatment of osteoporosis. It took the changes in serum estrogen, bone density, calcitonin/parathyroid hormone, thyrotropic hormone, free T3 and free T4 as the measuring indexes. Immunocyto-chemistry stain and morphological measurement of TSH cell,follicular adenoma of thyroid gland and thyroid globulin were applied in pituitary gland and thyroid gland to observe the therapeutic results.

采用注射地塞米松建立骨质疏松模型，用抗骨松穴位贴剂(antagonism osteoporosis acupoint paste，AOAP)外贴穴位、非经非穴位、并与口服补肾方药比较治疗骨质疏松，以血清雌激素、骨密度、降钙素/甲状旁腺素、促甲状腺激素、游离T3、游离T4的变化为衡量指标，取垂体、甲状腺作促甲状腺激素细胞、C细胞和Tg等免疫细胞化学染色，并形态计量，观察其治疗效果。

Methods: the expression of hALK in HepG2 cells was observed immunocytochemistry and reverse transcriptive PCR. Proliferation of HepG2 cells after neutralization was detected by 3H-TdR incorporation approach.

用免疫细胞化学以及RT-PCR的方法检测HepG2细胞hALR蛋白及mRNA的表达；用3H-TdR掺入法检测hALR特异性单克隆抗体中和hALR后HepG2细胞的增殖情况。

MethodsHuman embryonic lens epithelial cells were cultured by the explanting culture methods. An inverted phase contrast microscope, electron microscope and immunocytochemistry were used to study the growth specialties of the human embryonic lens epithelial cells.

方法组织块贴壁法培养人胚晶状体上皮细胞，通过倒置相差显微镜、透射电子显微镜，采用免疫细胞化学法分析人胚晶状体上皮细胞的生长特性。

After natural purification and three passaging, we detected CD90 expression in fibroblasts grown on cover glass by SABC immuocytochemistry techniques.

经过3次传代、自然纯化后得到性质稳定的成纤维细胞，冻存后复苏传1-3代，制成细胞爬片，免疫细胞化学SABC法染色检测CD90的表达情况。5。

objective to study the effects of berbamine and batiolun on ldh metabolizability in the process of increasing leukocyte.methods enzyme biochemical techniques is used to study the effect of berbamine and batiolˉun in the process of inceasing leukocyte and also to study the effect of ldh metabolizability.results when leukocyte is inhibited by the immune inhibitor cycolophosphamcide,berbamine and batiolun are compared of the effect of ldh activity.conclusionthe study draws the conclusion that both berbamine and batiolun can increase thegranule cell,and they also have some effect of the ldh metabolizability.

目的 研究小檗胺和鲨肝醇升高白细胞作用及对细胞内ldh的代谢的影响。方法采用酶细胞化学方法，对两种药物在治疗由免疫抑制剂环磷酰胺所致的白细胞减少症时，对乳酸脱氢酶活性的影响进行了比较观察。结果小檗胺和鲨肝醇均可升高粒细胞，同时对细胞内ldh的代谢有一定的影响。结论小檗胺和鲨肝醇能使外周血粒细胞增加，可用于治疗多种因素引起的白细胞减少症。

Control group was also set up.Then the AO/EB fluorescence double-dye technique,immunocytochemistry staining,Fluo-3 staining and Rhodamine 123 uptake assay approaches were adopted to observe the apoptotic rate,expression levels of Bcl-2 and Bax proteins,concentration of cytoplasmic free Ca2+ and mitochondrial transmembrane potential of NCI-H460 cells.

采用AO/EB荧光双染、免疫细胞化学染色、Rhodamine 123摄取法及Fluo-3/AM染色分别观察NCI-H460细胞的凋亡、Bcl-2及Bax蛋白的表达量、线粒体跨膜电位及细胞内游离钙离子浓度。

Methods: Myoblasts isolated from rat muscle samples were cultured and purified clonally in vitro. The 3rd passage cells were incubated with medium including basic fibroblast growth factor and dimethyl sulfoxid.

体外分离培养大鼠成肌细胞并克隆纯化，培养至第3代时加入含有碱性成纤维细胞生长因子和二甲基亚砜的培养液诱导分化，进行RT-PCR分析和免疫荧光细胞化学染色鉴定。

After 3-8 passages, the oral CAFs and the normal mucosa fibroblasts from tissue culture were observed under light microscope. Vimentin,α-SMA and Cytokeratin protein were detected by immunohistochemistry SP method on glass coverslips. VEGF, TGF-β1, MMP-9, Tenascin-C mRNA were analyzed by semi-quantitative RT-PCR.

用组织块培养法获得原代CAFs和正常粘膜成纤维细胞，0.25%胰蛋白酶消化传代和纯化细胞；通过形态学观察和免疫细胞化学染色对口腔CAFs和NMFs进行鉴定：采用半定量RT-PCR检测口腔CAFs和NMFs 4种基因的表达。

Elastase is an enzyme that shortens a cellular chemical, cyclin E, that is involved in controlling the cell cycle.

弹性蛋白酶是一种酶，缩短细胞化学，细胞周期蛋白E ，这是参与控制细胞周期。

Results After 2 weeks, the number of skeletal myoblasts reached 107 per millilitre. Immunocytochemical analysis showed that over 90% cells were stained positive for myogenin, a factor specific to skeletal myocytes.

结果 采用组织块培养法，2周后成肌细胞的密度可达107/ml，免疫细胞化学分析显示90%以上的细胞呈骨骼肌特异的myogenin抗体染色阳性。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。