细胞化学

Methods: The tissues were obtained from VZ/SVZ zones of lOw human embryon and dismissed with a polish-Pasteur pipette.

对培养的细胞用免疫细胞化学方法检测Nestin和PCNA，结合细胞培养传郑州大学2003届硕士研究生毕业论文代和分化情况，鉴定NSCs。

Methods To use Ce3+ or Pb2+ as capture agents to detect carbonic anhydrase, Na+-K+-ATPase, Mg2+-ATPase, 5' –nucleotidase and NADPH oxidase activities on endosome membrane in epithelial cells of renal proximal tubules、hepatic cell of rat and human leukocytes. with cytochemical method.

用细胞化学方法，以Ce3+或Pb2+作捕捉剂，分别检测碳酸酐酶，Mg2+-ATP酶，Na+- K+-ATP酶，5'-核等酸酶，NADPH oxidase在大鼠肾近曲小管上皮细胞、肝细胞以及人体嗜中性白细胞内吞体膜上的酶活性。

Methods:Special culture was performed in the fetal rat ependyma or adult rat bone marrow,respectively.

以细胞克隆及免疫细胞化学方法判断NSC增殖并鉴定NSC、神经元和神经胶质细胞。

DC have been studied by using the cytochemical methods. The results showed that the cytoplasm of the haustorial cells, the degenerating sites of integumentary cells and nucellar cells surrounding haustorial cells showed strong PAS staining.

DC的胚乳吸器进行了细胞化学研究，结果显示，胚乳吸器的细胞质、胚乳吸器周围解体的珠心细胞和珠被细胞均呈强PAS正反应。

Malpighian tubule, fat body, and stigma of the fifth instar larvae and were analyzed by Western blotting. The results showed that BmRRM only existed in seminal gland, epidermis and fat body.

以家蚕Bm5细胞进行免疫细胞化学实验，BmRRM蛋白在整个细胞周期主要分布于细胞核，仅少量分布于细胞质。

METHODS: NSCs were isolated from neonatal rats by enzyme digestion and mechanical separation. At the fourth passage, cell clone masses received nestin immunocytochemistry. Remaining cells were dispersed by mechanical separation. Monoclone NSCs were incubated by limiting dilution assay, and made into 108 L-1 monoplast suspension in complete medium. NSCs were assigned into 2 groups. NSCs in the control group were incubated in 10% fetal bovine serum.

酶消化和机械分离法相结合体外分离培养新生鼠神经干细胞，传至第4代的细胞克隆团行巢蛋白免疫细胞化学染色观察，剩余细胞团用机械法分散，采用有限稀释法进行单克隆神经干细胞培养，加入完全培养基制成108 L-1的单细胞悬液，分为2组滴入培养板，对照组加入10%FBS，诱导组加入10%FBS+50 μg/L神经生长因子，培养5~7 d。

To study the role of FoxO1 on myoblast proliferation and differentiation and the transformation of muscle fiber type in porcine,here we use yorkshire as experimental materials and detect the expression of genes related myogenesis and muscle fiber type in pporcine myoblast that was knockout or activated FoxO1,adopting the technology of RNAi,real time-PCR and Western blotting et al..

为探讨FoxO1对猪成肌细胞增殖分化及肌纤维类型转化的作用及机理，本试验以大白猪为研究材料，利用RNAi、real-time PCR、免疫细胞化学和Western blotting等技术，通过在原代培养的猪成肌细胞中敲除和激活内源活性FoxO1，检测肌肉形成与肌纤维类型相关基因的表达。

In situ hybridization and immunocytochemical studies indicated that treatment with 17beta -estradiol increased the number of aromatase-immunoreactive cells and their immunoreactivities, especially in the preoptic area of the hypothalamus, where the density of the aromatase-immunoreactive cells was 2.6 folds higher than the control group. Results indicated that brain aromatase may be involved in the feminization induced by 17beta -estradiol of the grey mullet.

原位杂交和免疫细胞化学定位表明：雌二醇处理组鲻鱼脑内芳香化酶阳性细胞数量和反应强度均提高，尤其在下丘脑视前区，芳香化酶阳性细胞的分布密度为对照组的2.6倍，说明17beta-雌二醇诱导幼鲻雌性化可能与脑芳香化酶的表达有关。

After changing medium, BMSCs were incubated in serum-free DMEM containing BRA and dimethyl sulphoxide for 5 hours to 6 days. MAIN OUTCOME MEASURES: Cell transfection was observed with a fluorescence microscope.

主要观察指标：荧光显微镜观察细胞转染情况，免疫细胞化学染色检测转染细胞诱导后神经元表面抗原的表达。

The proliferation of cultured rat renal fibroblasts was measured by means of methylthio tetrazole method.

用重组PTEN腺病毒转染体外培养大鼠肾成纤维细胞，MTT法检测细胞增殖活力，免疫细胞化学检测PCNA蛋白表达。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。