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细胞分裂素

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The temporal andspatial expression of CaM mRNA may be closely related with cell division, pollendevelopment and substance transport.

烟草花药发育过程中钙调素基因的时空表达可能与细胞分裂、花粉发育和物质转运密切相关。

The unique caterpillar fungus element, which is antibiotic and restrictive of cancer cell division, can restrain cancer cell multiplication.

其独有的虫草素,具有抗生作用和抑制细胞分裂作用,能抑制癌细胞的增生。

Phosphorus is associated with the protoplasm of the cell nucleus and lack of it retards cell division and plant development.

磷素与细胞核原生质有关,所以缺磷势必影响细胞分裂和植物的发育。

Phosphorus is associated with the protoplasm of the cell nucleus and lack of it retard s cell division and plant development.

磷素与细胞核原生质有关,所以缺磷势必影响细胞分裂和植物的发育。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果:1、哇巴因作用于晶状体钠泵后,光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙,电镜下全层细胞空泡化、线粒体肿胀出现髓样结构,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后,光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离,电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后,光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整,电镜下细胞器丰富、细胞功能旺盛,RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后,光镜下晶状体质膜完整、细胞排列紧密规整,电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝,RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后,光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化,电镜下囊侧膜内有高电子密度带、细胞核仁丰富,RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后,光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏,电镜下质膜变性、线粒体肿胀,RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

Methods Cell cultures were harvested and stained with haematoxylin and eosin,to analyze cellular metaphase morphology.

收获细胞经苏木素和伊红染色,观察分裂中期细胞核形态,计算核极分裂象细胞百分比。

Cultured cells were harvested and stained with haematoxylin and eosin to analyze cellular metaphase morphology.

收获细胞经苏木素和伊红染色,观察分裂中期细胞核形态,计算核多极分裂像细胞百分比。

Methods Cell cultures were harvested and stained with haematoxylin and eosin,to analyze cellular metaphase morphology.

收获细胞经苏木素和伊红染色,观察分裂中期细胞核形态,计算核多极分裂象细胞百分比。

NAA, CPPU and EBR all induced parthenocarpic growth together with active cell division and enhanced transcripts of cell cycle-related genes.

奈乙酸,N-(2-氯-4-吡啶基)-N-苯基脲和表油菜素内酯都能诱导黄瓜单性结实,加快细胞分裂和提高细胞周期相关基因的表达。

These include depolarization of the membrane potential in epidermal cells, specific expression of early nodulin genes and changes in the flux of calcium in root hairs, deformation of root hairs, cell division in the root cortex and formation of the nodule primordium.

结瘤因子信号能够诱导豆科植物根毛细胞质膜去极化、离子流动和早期结瘤素基因的表达以及根毛变形、皮层细胞分裂和根瘤原基形成等与共生有关的表型变化。

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