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细胞分裂

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On low-magnification light microscopy, the specimen had a patternless tissue culture-like appearance with increased mitotic actiity, randomly oriented spindle cells, and extraasated red blood cells (Figure 2B ).

低倍光学显微镜下,标本显示无图案的&组织培养样&表现,伴有有丝分裂活性增高、未定性的梭形细胞和红细胞外渗(图2b),组织病理学所见符合结节性筋膜炎。

It has been known that the contour of the nucleus of some malignant cells was not regular, and the perinuclear cisternae as well as the internal dense lamillae was thicker than that of the normal cells.

可见,肝癌等恶性肿瘤细胞核膜表面积与核体积的比例显著大于对照肝细胞,约增长了40%,定量证实了肿瘤细胞核膜可随不断增殖分裂的需要而增加其核膜表面积。

The perivitelline space was narrow at GV stage and there were many microvillus penetrating zona pellucida between oocyte and cumulus cells.

在减数分裂启动过程中,小鼠卵母细胞内也发生相应的形态学上的变化。

Detect with Western Blot protein of MAb counterpoise form and the natural phosphoric acid is enzymatic reactivity in the cell, obtain 6 individual plant to be able to be stabilized in all secrete the individual plant of cross tumour cell that fights phosphoric acid enzymatic MAb, go enzymaticly to study double particularity phosphoric acid further phosphation action mechanism and its activation formerly in mitosis way of enzymatic signal of albumen phosphoric acid turns guide provide strong tool.

用Western blot检测mAb对重组蛋白和细胞中天然磷酸酶的反应性,共获得6株可稳定分泌抗磷酸酶mAb的杂交瘤细胞株,为进一步探究双特异性磷酸酶的去磷酸化功能机理以及其在有丝分裂原激活蛋白磷酸酶信号途径中的信号转导提供强有力的工具。

The reseach on activity changes of SOD, POD and CAT during the somatic embryogenesis of Y35 showed:(1) The activty of SOD was from 52.98 to 133.20 U·g-1·h-1, and remained a rising trend after early single embryo forming, this revealed that SOD might be positively correlated to the differentiation of embryogenic cell and the development of somatic embryo.(2) The activty of POD was from 0.05 to 0.50 U·mg-1·min-1, ascended firstly and desceded later, and was highest in embryogenic callus and lowest in late single embryo , this revealed that POD might be positively correlated to the division and differentiation of proembryo mass, while negatively correlated to the development of PEMⅢto late single embryo.(3) The activty of CAT was from 0.86 to 2.81 U·mg-1·min-1, showed an up-down-up trend, reaching to the highest peak at the time of early embryo formating and decreasing to the lowest at the time of early cotyledonary embryo formating, this revealed that CAT might be positively correlated to the development of early single embryo, while negatively correlated to the formation of middle single embryo and early cotyledonary embryo.The changes in activty of SOD, POD and CAT indicated these three antioxidant enzymes coregulated the differentiation and development of embryogenic cells during Larix somatic embryogenesis.4. Differentially expressed cDNA libraries of the stages of proembryo mass and somatic embryo maturation were successfully constructed by suppression subtractive hybridization.

对Y35体细胞胚胎发生过程中抗氧化酶活性变化的研究显示:(1)SOD活性在52.98~133.20 U·g-1·h-1之间,并在早期单胚形成后一直保持上升的趋势,表明其与胚性细胞的分化及体细胞胚的发育均具呈正相关;(2)POD活性在0.05~0.50 U·mg-1·min-1之间,呈现出先下降后升高的趋势,在胚性愈伤组织中最高,而在后期单胚形成时降至最低,表明其与原胚团的分裂和分化呈正相关,但与PEMⅢ向后期单胚的发育呈负相关;(3)CAT活性在0.86~2.81 U·mg-1·min-1之间,表现出升-降-升的变化趋势,在早期单胚形成时升至最高,在早期子叶胚形成时降至最低,表明其与早期单胚的发育呈正相关,而与中期单胚和早期子叶胚的发育呈负相关。

In this study, a new horizontal microinjection system has been established to solve these problems. Because the average time per pronuclear embryo by microinjection has been significantly shortened with the horizontal system, the cleavage rate and 16-cell rate of those microinjected embryos increased markedly.

为了解决这些问题,本研究有针对性地设计了新型的水平微注射系统,利用此系统,可明显缩短常规注射原核胚的平均时间,注射后原核胚的分裂率、8-16细胞胚胎发育率也明显提高。

Cell wall formation and division of protoplasts derived form E. radiata suspension cells were observed. However, division did not progress beyond this stage and the protoplasts died after one month.

来自放射桉悬浮细胞之原生质体培养,可观测到原生质体分裂及细胞壁的形成,唯经培养1个月后,并未再进一步的发展而渐死亡。

These abnormal changes mainly occurred at two stages:(1) at meiotic prophase, almost half of Peiai 64S microsporocytes exhibited aberration, with sparse free ribosomes, underdeveloped mitochondria and many swollen endoplasmic reticula.

这些变化主要发生在两个阶段:(1)减数分裂前期,约半数的小孢子母细胞的胞质出现异常,游离核糖体稀少,并具有不发育线粒体和大量泡状内质网。

Firstly, the parallel growth inhibition experiment of S. pombe wild-type cells, spm1△, pmp1△, mid2△and relevant retransform strains were carried out in order to farther confirmed the effect of edelfosine on spm1, pmp1 and mid2 genes.

因此,本实验的目的是探讨依地福新对经Mid2介导的,Pmp1抑制的MAPK Spm1信号通路的影响,以阐明依地福新抑制粟酒裂殖酵母细胞胞质分裂的作用机制。

The microtubule cytoskeleton was investigated by using cryosectioning and indirect immunofluorescence staining in the shoot apex of Saccharum spontaneum.

利用冰冻切片法结合间接免疫荧光标记技术对割手密茎尖细胞有丝分裂过程中微管骨架的变化进行了研究。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

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