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细胞分裂

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We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果:体外实验结果表明:一定浓度的硒作用HP后,HP菌落数量随着硒浓度的增加而减少,菌落颜色由灰白色变为砖红色,溶血现象随硒浓度的增加和作用时间的延长而逐渐消失,HP的形态由弯曲形变为球形或椭圆形,鞭毛及菌毛出现脱落现象;硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显;一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素,颜色随硒浓度增加而加深;一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长,减少重组VacA表达量,但不影响其空泡毒活性及编码基因序列;硒能增强重组VacA对胃癌细胞的抑制作用,使细胞分裂指数和集落形成率降低、S期细胞数量减少,G1期细胞总数增加;纯化后的VacA重组蛋白可被VacA抗体识别,具有良好的抗原性。

The ultrastructural changes during the development of sieve element companion cell complex (SE/CC complex) in Populus deltoides were investigated using electronic microscopy. The result showed that SE and CC tome from the same secondary phloem mother cell. The development of sieve element went through three stages, i. e. immature, mature, and degenerate stage. In immature stages the sieve element presented radial expansion, cell wall thickening, and production of sieve plastid and P-protein. The cytoplasma selectivly autolysed in mature stage. At the same time, karyoplasm was dispersed, and nuclear envelope was not clear, or karyoplasm was disorgnized, and nuclear envelope was clear. The karyopalsm finally turned into P-protein. In degenerate stage, the cytoplasm completely disappeared, and the function was lost.

利用电镜技术研究美洲黑杨次生韧皮部筛管和伴胞发育过程中细胞超微结构的动态变化,观察到筛管和伴胞由同一个次生韧皮部衍生细胞分裂分化形成筛管约发育历经未成熟期、成熟期和衰退期的变化未成熟期筛管分子表现为细胞的径向扩展、壁的增厚、筛管质体和P-蛋白质的产生;成熟期筛管分子细胞组分发生选择性自溶,细胞核核质弥散状,核膜不清晰,或核膜清晰而核物质降解,最后细胞核完全解体转化为P-蛋白质类的物质;衰退期筛管分子细胞质完全解体消失,失去功能。

Obviously, large amounts of cyclin B1 existing in spermatids has no effect on adjusting the activity of 〓 kinase, which may be just a signal of the end of cell division and/or a necessary factor for the initiation of the metamorphosis of spermatids. Abdominal high temperature inhibit efficiently the translation of cyclin B1 and 〓 in the spermatogonia and pachytene/diplotene primary spermatocytes.

显然,精子细胞中大量存在的cyclin B1蛋白不会起到调节〓激酶活性的作用,而是作为一种细胞分裂完成的信号,为启动精子细胞变态所必需;(4)腹腔高温使粗线期/双线期初级精母细胞中cdc2和cyclin B1转录本的翻译受阻。

After removal of the episome, iPS cells completely free of vector and transgene sequences are derived that are similar to human embryonic stem cells in proliferative and developmental potential.

在质粒中的基因所表达的蛋白质会将细胞重新编程并使其成为iPS细胞。这些iPS细胞在接着的几轮细胞分裂中会开始失去这些质粒,这样研究人员就可以分离出不含质粒的细胞。

The immune level tested in different time after injection with ginkgolic acids in sensitization mode showed that the expression of CD3〓 and CD4〓T lymphocytes increased, but CD8〓 was restrained, while the B lymphocytes was also increased. The results suggested that sensitivity was approved and T lymphocytes proliferation was induced, which strength cell immunity responsion, at the same time B lymphocytes was differentiated to IgE antibody-producing cells when contacted with ginkgolic acids as allergen.

大鼠经致敏方式接触银杏酸后,在不同时间的细胞免疫水平与体液免疫水平的研究结果表明,大鼠CD3〓及CD4〓T淋巴细胞的表达增加,而CD8〓T淋巴细胞的表达受到明显抑制,同时参与体液免疫的B淋巴细胞百分率呈增高趋势,提示银杏酸可能作为变应原使机体敏感性增强,诱导T细胞分裂增殖,增强细胞免疫应答,同时促进B细胞增殖分化成IgE抗体产生细胞。

The secondary xylem of woody plants is a product of the vascular cambium,its formation is a complicated type of plant cell differentiation, including cambium initiation, cambial cell division, cell expansion, secondary wall deposition and programmed cell death.

木本植物的次生木质部是维管形成层的活动产物,其形成过程是植物细胞分化的一种复杂类型,包括维管形成层发生、形成层细胞分裂、分化、细胞扩展、次生壁形成和细胞程序化死亡等阶段。

The mechanism of Si Junzi Tang and panax sapogenol were studied on the effect of gastrointestinal cells protection by applying advanced technology of histochemistry, Holt′s cytodieresis mircocirculation, radio-immunity and subcellular ultrastructure.

进而应用酶组织化学、Holt细胞分裂测定、微循环测定、放射免疫测定、亚细胞超微结构观察等先进技术,研究四君子汤、人参皂甙抗胃肠细胞损伤、保护胃肠细胞的作用机理。

The nucleus of mature egg cell was located at the micropylar end and a great deal of starch grains were in the cytoplasm surrounding the nucleus.

胚囊发育为蓼型,反足细胞分裂形成反足组织,反足组织含有大量淀粉粒,反足细胞直到胚乳细胞化时才退化,反足细胞在胚和胚乳发育中起着积极作用。

SCs divided and proliferated in three-dimensional fashion, demonstrating long olivary, triangular or long fusiform shape with obvious prominence. Furthermore, The SCs connected end-to-end with each other or aligned in clusters and the protein granules secreted on cellular surface were also showed.

雪旺细胞分裂增殖呈三维生长,细胞形态多为长梭形,纺锤形或长三角形,突起显著,细胞呈端对端的相互连接或排列成束状或栅栏状,细胞表面蛋白颗粒分泌良好。

At the same time, the process of the wound healing was observed histologically, and the regeneration cycle of epidermal cells and the temporal change in inflammatory cells were measured. Results Inflammatory cells infiltrated into wound surface were neutrophils, followed by macrophages and lastly lymphocytes. Epidermal cells proliferated most actively on PBD 3 and the mitoses of them increased significantly on day 7 after burn. The gene expression of PDGF, PDGFR and EGFR reached peaks on PBD 1 and the gene expression of EGF and TGFβ-R2 were highest on PBD 3. In addition, the gene expression of TGFβ-R1 and TGFβ1 increased significantly on PBDs 5 and 7 respectively.

结果 炎性细胞到达创面依次为中性粒细胞、巨噬细胞和淋巴细胞;表皮细胞周期变化为伤后3天细胞增殖活跃,伤后7天细胞分裂明显增多;伤后1,3天PDGF、PDGFR(血小板源性生长因子受体)和EGFR基因表达明显增强,伤后3,5天EGF、TGFβ-R2基因表达增强,伤后5,7天TGFβ-R1(转化生长因子β受体1)基因表达增强,伤后7,10天TGFβ1基因表达增强。

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