细胞分裂

Sowe selected the colchicine optimum parameters were 0.6μg/ml and 6 hour culturedduration.The second, there were no significantly differences between the four groupsin 2 hour groups; and there were significantly differences between 0.2μg/ml groups and other three groups in 4 hour groups; and there were no significantlydifferences between the four groups in 6 hour groups; and there were significantlydifferences between 0.4μg/ml groups and other three groups in 8 hour groups onthe metaphases (P＜0.05), in the experiment of the effect on the mouse 8-cellembryo stage single blastomere of colchicine with different concentrations andduration by x~2 statistical analysis.

根据实验的实际情况，适宜制备小鼠4-细胞期胚胎单卵裂球染色体标本的秋水仙素的处理浓度和时间是0.6μg／ml和6小时。2、在确定秋水仙素不同处理浓度和时间对小鼠8-细胞期胚胎单卵裂球中期分裂相数影响的实验中，经统计分析，阻断培养2小时组中四个浓度组差异均不显著；阻断培养4小时组中0.2μg／ml浓度组与其他三组差异显著；阻断培养6小时组中四个浓度组差异均不显著；阻断培养8小时组中0.4μg／ml浓度组与其他三组差异显著(P＜0.05)。

So we selected thevinblastine optimum parameters were 0.1μg/ml and 8 hour cultured duration.The fourth, there were significantly differences percent between 10 hourgroups and other three groups in 0.1μg/ml groups; and there were no significantlydifferences between 8 hour groups and 10 hour groups, but they had significantlydifferences among other two groups in 0.3μg/ml groups; and there weresignificantly differences between 10 hour groups and other three hour groups in0.5μg/ml groups; and there were significantly differences between 10 hour groupsand other three groups in 0.7μg/ml groups on the metaphases (P＜0.05), in theexperiment of the effect on the mouse 8- cell embryo stage single blastomere ofvinblastine with different concentrations and duration, by x~2 statistical analysis.

根据实验的实际情况，适宜制备小鼠4-细胞期胚胎单卵裂球染色体标本的长春花碱的处理浓度和时间是0.1μg／ml和8小时。4、在确定长春花碱不同处理浓度和时间对小鼠8-细胞期胚胎单卵裂球中期分裂相数影响的实验中，经统计分析，阻断培养0.1μg／ml浓度组中10小时组与其他三组差异显著；阻断培养0.3μg／ml浓度组中8小时组和10小时组间差异不显著，但与其他两组差异显著；阻断培养0.5μg／ml浓度组中10小时组与其他三组差异显著；阻断培养0.7μg／ml浓度组中10小时组与其他三组差异显著(P＜0.05)。

So we selected the colchicine optimumparameters were 0.2μg/ml and 6 hour cultured duration.The third, there were significantly differences percent between 10 hourgroups and other three groups (4 hour groups, 6 hour groups and 8 hour groups) in0.1μg/ml groups; and there were significantly differences between 8 hour groupsand 10 hour groups, but they had no significantly differences among other twogroups in 0.3μg/ml groups; and there were significantly differences between 10hour groups and 6 hour groups, but 10 hour groups had no significantlydifferences among other two groups in 0.5μg/ml groups; and there weresignificantly differences between 8 hour groups and other three groups in0.7μg/ml groups on the metaphases (P＜0.05), in the experiment of the effect onthe mouse 4- cell embryo stage single blastomere of vinblastine with differentconcentrations and duration, by x~2 statistical analysis.

根据实验的实际情况，适宜制备小鼠8-细胞期胚胎单卵裂球染色体标本的秋水仙素的处理浓度和时间是0.2μg／ml和6小时。3、在确定长春花碱不同处理浓度和时间对小鼠4-细胞期胚胎单卵裂球中期分裂相数影响的实验中，经统计分析，阻断培养0.1μg／ml浓度组中10小时组与其他三组差异显著(4小时组、6小时组和8小时组)；阻断培养0.3μg／ml浓度组中8小时组和10小时组间差异不显著，但与其他两组差异显著；阻断培养0.5μg／ml浓度组中10小时组与6小时组差异不显著，而与其他两组差异显著；阻断培养0.7μg／ml浓度组中8小时组与其他三组差异显著(P＜0.05)。

Sowe selected the vinblastine optimum parameters were 0.1μg/ml and 8 hourcultured duration.The fifth, there were significantly differences between 0.45% sodium chloride groups and other two groups (0.5% sodium citrate groups and 0.35%potassium chloride groups) on the metaphases (P＜0.05), in the experiment of theeffect on the mouse 4-and 8-cell embryo stage single blastomere of differenthypotonic fluids, by x~2 statistical analysis.

根据实验的实际情况，适宜制备小鼠8-细胞期胚胎单卵裂球染色体标本的长春花碱的处理浓度和时间是0.1μg／ml和10小时。5、在确定不同种类低渗液处理对小鼠4、8-细胞期胚胎单卵裂球中期分裂相数影响的实验中，经统计分析，0.45％氯化钠低渗液组与其他两组(0.5％柠檬酸钠和0.35％氯化钾)差异显著(P＜0.05)。

BACKGROUND: Basic fibroblast growth factor can accelerate the bone marrow mesenchymal stem cells proliferation and differentiation into nerve cells, which is considered as a mitogen of glial cells.

背景：碱性成纤维细胞生长因子可以促进骨髓间充质干细胞的增殖和向神经细胞方向分化，并被认为是胶质细胞的分裂原。

Fibroblast growth factor is a kind of the polypeptide growth factors, which is known to be a mitogen for a wide variety of mesoderm-and neuroectoderm-derived cells and a neurotrophic factor for several neuronal cell types.

成纤维细胞生长因子是一种对中胚层及神经外胚层源的细胞都有促分裂活性的多肽生长因子，也是一种重要的神经营养因子。

Objective: To express recombinant human endostatin in yeast and study its mitogenic activity on 3T3 fibroblast cells.

摘 要 目的：利用酵母真核细胞表达系统表达出重组人内皮抑制素，并对其促成纤维细胞有丝分裂活性进行研究。

Results showed that Vero cells grew synchronously by treating the double thymidine block method.

在Vero细胞的一个分裂周期中，细胞对底物的消耗和产物的生成有一定的周期行为。

The results showed that the irradiation of γ-rays inhibited mitoses and produced various aberrations, such as single micronucleus, double micronucleus, multi-micronucleus, small micronucleus, chromosome fragment, chromosome conglutination, single bridge, double bridge, multi-bridge, dissociative chromosome, lagging bridge in root tip cells of Medicago sative L.

实验结果表明，γ射线辐照苜蓿种子可以抑制根尖细胞有丝分裂，并诱发根尖细胞产生单微核、双微核、多微核、小核、染色体断片、染色体粘连、单桥、双桥、多桥、游离染色体、落后染色体等多种畸变。

In richer places, fewer runts died, and the daughters with shiny, new bits grew and divided very rapidly indeed.

在肥沃的地方，只有少数的弱者死去，子细胞生龙活虎，新细胞成长的更快分裂的也快。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。