细胞分裂

Using cytohischemical staining methods, with the results of comparison the dynamics of proteins and polysaccharides in the anthe wall cells and colule cells of different developmental phases in female and male flowers, the anormogenesis of anther tapetum in the inflecting phase of from bisexual flower to unisexual flower was observed. In microspore developed phase, the tapetum functions of preserving and transportingmutritive material for microspore development and of secreting callosal enzyme for decompositing callosal cell lost no normal guadrant formed, of being abnormal meiose of pollen mother cells, and then, the stamen aborted selectively in female flowers and pistil in male flower.

利用细胞组织化学染色技术，对雌、雄花雄蕊花药壁细胞及花药内细胞发育过程中多糖及蛋白质动态进行了比较，实验中观察到雌花在从两性至单性花转变时期雄蕊绒毡层在整个发育过程中表现异常，在小孢子发育过程中未能起到贮藏、转运营养物质供小孢子发育及适时分泌胼胝质酶溶解胼胝质壁的功能，并且花粉母细胞减数分裂异常而未形成四分体结构，进而导致雌花雄蕊选择性败育，而雄花中雌蕊组织也发生了选择性败育过程。

Experimental study indicated: the flavone of rhizome drynaria can improve the cytoactive of marrow stroma cell of rats, promote its multiplicative division, and protect the marrow stroma cells' apotosis with lipidsion.The flavone of rhizome drynaria's serum can extently protect the osteoblast apoptosis of rats induced by TNF-α and the osteoblast senescence of rats induced by t-BHP.

实验研究表明：骨碎补总黄酮能够提高大鼠骨髓基质细胞的活性，促进其增殖分裂，并对脂质化大鼠骨髓基质细胞凋亡具有保护作用，而且骨碎补总黄酮含药血清对TNF-α诱导的大鼠成骨细胞凋亡和t-BHP诱导的大鼠成骨细胞衰老均具有一定的保护作用。

Results indicated that suppression of malignancy was observed in the hybrid cells, they became differentiated morphologically and functionally by:(1) changes in histochemical staining, nucleus-cytoplasm in ratio, and increase in cell size and nucleal heterochromation, but decrese in cell surface microvilli and number of nucleoli;(2) decrease of growth rate, mitotic index and DNA synthesis rate;(3) loss of the ability to growth in soft agar medium, and non-tumorigenic under heterotransplantation to nude mice;(4) reduction of chromosome number mainly of rat derivative and decrease in chromosomal aberration rate.

实验结果表明：（1）杂交细胞出现瘤细胞与网织红细胞杂交同样的表型逆转及去恶性化特征，主要表现在细胞体积增大、核质比例减小；DNA合同速率降低、分裂指数下降，细胞生长显著减慢；在软琼脂中不形成集落，在裸鼠体内失去了长瘤能力。

Results ⑴ The cell proliferation was inhibited by TET. It showed thatdivision index and cell number during G0-G1 were decreased, while S and G2-M phases were lengthened with dosage and time dependency in dosages of TET from 2mg/L to 8mg/L; besides, oval-shap cells, karyopyknosis and fragmentation were observed under light microscope. The inhibitory effect in T2 group was statistically similar to that in triamcinolone.

结果⑴ TET在2mg/L~8mg/L之间呈剂量及时间依赖性地抑制瘢痕成纤维细胞的增殖，使生长曲线不同程度的压低、分裂指数降低、G0-G1期细胞数减少、G2-M期增多、S期与G2-M期延长，光镜下见细胞突起变短，细胞由梭形变成椭圆形，核固缩、碎裂。P.C的改变近似于T2组(4mg/L TET)。

The proliferating cells were mainly distributed in the peri-chondrium and the peripheral zone of tracheal cartilage. The senescent chondrocytes were distributed in the central zone whereapoptotic morphological characteristics obviously appeared, such as pyknosis, karyorrhexis.

结果：软骨细胞呈现多种方式的无丝分裂像；气管软骨膜与软骨周围带多见细胞增生；气管软骨中央带多见衰老的软骨细胞，并见明显核固缩、核碎裂等细胞凋亡形态学特征。

And the conditions in cell suspension culture of Mikania micrantha were studied. The results showed that sucrose was the compatible carbon sucrose, and 30g/L sucrose concentration can satisfy the growth of Mikania micrantha cell; ammonium was absorbed under different sucrose concentration that haven't demonstrated significant specificity, and was completely absorbed on the lag phase and the early logarithmic phase; while nitrate was mainly absorbed on logarithmic phase. The density-dependent of Mikania micrantha cell starting to grow and density-inhibited of cell growth were proposed, the fittest inoculating quantity of Mikania micrantha in cell suspension culture was 40g/L.

并对微甘菊细胞悬浮培养条件进行研究，结果表明，微甘菊细胞生长适宜的碳源是蔗糖，并且较合适的蔗糖浓度为30g／L；在不同蔗糖浓度下氨基氮的吸收差别不大，氨基氮在迟滞期和对数期前期已基本消耗完毕，对数期生长主要利用硝基氮，因而提出，在微甘菊细胞液体悬浮培养中，氮源可以采用初始低氨基氮浓度，对数期中间维持高硝基氮，而且对数期中逐步流加少量的氨基氮；微甘菊细胞迟滞期分裂启动存在密度依赖现象，对数期生长存在密度抑制现象，最适接种量为40g／L。

The results showed that:(1) Many proliferating cells were distributed unevenly in the ventricular zone of telencephalon, and the [3H] labeled cells were found to be populated in the lateral ventricular zone of neostriatum at brain level L12~L14,forming proliferation "hot spots";(2) Having underwent mitosis twice, the proliferative cells in the VZ began to migrate laterally 6 days after intramuscular injection of [3H] thymidine, and these migrating precursor cells terminated at their targets and differentiated into new neurons on the 30th day.

结果发现：①端脑室带区中存在不均匀分布的[3H]标记细胞，大量标记细胞分布在端脑尾侧新纹状体水平(L1～L14)的VZ，形成标记细胞的&热点&区；②标记细胞经两次有丝分裂后，大约在肌肉注射[3H]的第6天后开始从VZ向外迁移，在30天左右到达目的脑区并分化成为成熟神经元。

The results showed that the rates of mature oocyte were 75.2％, 73.1％, 69.8％, 63.5％and oocyte at telophase of MI were 16.3％, 15.9％, 16.9％, 27.0％, respectively. The rates of maturation of oocytes cultured in TCM199 with serum, EGF and TGFαwere significantly higher than that of with BSA team (P＜0.05) and the number of oocyte stayed at telophase of MI in TCM199 with serum, EGF and TGFαteam were significantly less than that with BSA team(P＜0.05); Significant higher rates (66.6％, 66.6％, 73.6％) of normalα-tubulin distribution in oocytes cultured in TCM199+ serum, EGF and TGFαwere compared to that of TCM199+BSA(43.3％)(P＜0.05). The rates of oocyte with cortical granules in cortex were 58.8％, 33.9％, 54.7％and 47.9％respectively, there was significant difference between oocytes cultured with serum, EGF and BSA(P＜0.05). In conclusion, TGFαand EGF can promote the oocyte nuclear transition from telophaseⅠto metaphase of meiosisⅡ, and improved the expression and distribution ofα-tubulin during the ovine oocytes maturation; EGF and serum could promote oocyte cytoplasm maturation. The results suggested that EGF and TGFαmight substitute some substance in serum to improve the quality of oocyte nucleus maturation in vitro, but EGF might be more functional than TGFαto promote the maturation of ovine oocyte ooplasm.

三、EGF和TGFα对卵母细胞成熟的影响未成熟卵母细胞分别在TCM199基础培养液+血清、TCM199基础培养液+BSA+EGF、TCM199基础培养液+BSA+TGFα和TCM199基础培养液+BSA四组成熟培养系统中成熟培养，22小时后上述各组卵母细胞的核成熟率分别为75.2％、73.1％、69.8％、63.5％，处于第一次减数分裂末期的比率分别为16.3％、15.9％、16.9％、27.0％，在添加血清、EGF、TGFα各组中核成熟率显著高于其它各组(P＜0.05)，处于末期的比例明显低于其它各组(P＜0.05)：添加血清、EGF、TGFα各组在a-Tubulin蛋白的分布与表达上(66.6％、66.6％、73.6％)明显高于BSA组的正常率43.3％(P＜0.05)；各组CG发生迁移较好的卵母细胞的比率分别为58.8％、33.9％、54.7％、47.9％，血清组和添加EGF组CG迁移至皮质区的比例明显高于仅添加BSA处理组(P＜0.05)。

Vein transplantation of human UCB-MSCs has protective effects on splenocyte damages induced by irradiation, can promote proliferation and accelerate repair of splenocytes. The action mechanism is associated with elevating natural killer cell activity, and number of CD4+ and CD8+.

脐血间质干细胞静脉输注对辐射造成的脾细胞损伤有一定保护作用，可促进脾脏细胞的分裂增殖，加速脾脏细胞的修复，其作用机制可能与提高NK细胞活性和CD4+、CD8+数量有关。

But, there was no Ca(superscript 2+) precipitate in tapetal cells of Zhenshan 97B at the pollen mother cell stage and the meiosis stage and the Ca(superscript 2+) precipitates in tapetal cells mainly deposited in the disaggregated cytoplasm.

而保持系绒毡层细胞遮花粉母细胞时期和减数分裂时期细胞内没有Ca(上标 2+)沉淀；单核花粉时期绒毡层细胞内的Ca(上标 2+)沉淀主要分布在解体的细胞质内。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

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