细胞分裂

It is a complex process involving cell division, differentiation and interaction between cells in the microenvironment of the seminiferous tubule.

睾丸生精小管中的生精细胞先后经过三个阶段的变化：精原细胞的增殖，精母细胞的减数分裂和精子细胞的变态过程，最终产生成熟的精子。

Studies also indicated that genistein exsists not only in soybeans but also in many kinds of plants such as vegetables and fruits.As a potential anticancer agent, the actional mechanisms of genistein mainly includes as follows:First, genistein can depress the activity of protein tyrosine kinase and transduction pathways for the phosphorylation of receptors and mitosis signal. So genistein can lead to cells' proliferation depressed. Second, genistein has minimal effects of phytoestrogens. It can be combined with estrogen receptor and improve the synthesis of cellular sex hormone binding glulobin, and improve the activity of UDP-glucuronyl transferase. Through these pathways, it can inhibit the cell activity of breast cancer and prostate cancer.

作为一个很有潜力的抗肿瘤物质，三羟异黄酮的作用机制主要包括：①抑制蛋白酪氨酸激酶活性，可阻抑PTK引起的受体磷酸化和有丝分裂的信号传递，导致癌细胞增殖受抑；②弱雌激素作用：可通过与体内雌激素受体结合，并可增加细胞内性激素结合球蛋白的合成，增加UDP-葡糖醛酸转移酶的活性等途径抑制乳腺癌和前列腺癌细胞活性；③拓扑酶Ⅰ和Ⅱ抑制剂，抑制细胞活性；④上调细胞周期性负性调节因子P21WAF1/CIP1的表达，使之负性调节因子作用增强；⑤可阻止胰岛素样生长因子-Ⅰ、肝细胞生长因子和神经生长因子的作用而抑制肿瘤生长；⑥其他：抗氧化作用、抑制热休克蛋白、诱导细胞凋亡、抑制新生血管生成和抑制多种耐药相关蛋白等。

After cocultured with reactive astrocytes, olfactory ensheathing cells can promote the proliferation of reactive astrocytes,and reduce the astrocyte gliosis and expression of inhibitory cytokines.

嗅鞘细胞作用于活化后的星形胶质细胞以后，能够促进星形胶质细胞的分裂增殖，同时能够减少星形胶质细胞胶质化，降低抑制性细胞因子的表达。

Results Myoid/myofibroblastic differentiation occurred most commonly in fibrosarcomatous DFSP. It was recognized histologically as peripherally distributed or randomly scattered small eosinophilic nodules or short bundles, which were composed of bland spindle cells, closely resembling smooth muscle cells or myofibroblasts.

结果 肌样／肌纤维母细胞性分化多出现在纤维肉瘤型DFSP中，表现为肿瘤周边部或肿瘤内散在性分布的深嗜伊红色小结节或短条束，由梭形细胞组成，细胞多无异型性，核分裂象也罕见，形态上似平滑肌细胞或肌纤维母细胞。

The mature egg cell was an inactive cell with only a few polysomes. At the early zygote stage, a large number of ribosomal precursors were produced by the nucleolus, and many polysomes appeared in the cytoplasm, which suggests a high level of metabolism. Zygote at the dormancy stage had a small nucleolus and marked decrease in ribosomes, as shown by a few polysomes, which suggests decreased metabolism. Zygotes in the prophase of mitosis and two-celled proembryo became active again in metabolism, for a prominent nucleolus, high density of ribosomes and increased number of polysomes in the cytoplasm.

结果如下：在成熟卵细胞中多聚核糖体数量不多，且细胞代谢活性较弱；初期合子内，核仁大量合成核糖体前体物质，胞质中多聚核糖体数目众多，细胞代谢活性较强；休眠期合子的核仁变小，胞质中核糖体数量急剧减少，仅有少量多聚核糖体，细胞代谢活性较弱；合子分裂前期和二细胞原胚期，核仁显著，胞质中核糖体的密度增加，出现大量多聚核糖体，细胞代谢活性较强。

Results of the experimental groups: Comparison with the control groups, in 6h and 8h groups eminences of ependymal cells with cobblestone-like appearance were seen, their microvilli were further richer, and a number of microholes could be found on their base. In body part of SFO cell membrane of some ependymal cells were broken completely, their cytoplasm was no longer seen, and cell nucleus located at one side of the cell, 5-7μm in diameter and dividing lobe-like in shape. Beside those cells some ependymal cells appeared many folds on the top of surface. In 2h and 16h groups the observation results seemed no significant differences from the corresponding control groups.

实验组结果：与对照组相比，在6小时和8小时实验组中，可见成团的鹅卵石状室管细胞隆起于表面，微绒毛更为丰富，微绒毛根部可见到若干微细小孔，在SFO体部的一些室管膜细胞的胞膜完全破裂，胞质几乎荡然无存，仅剩胞核居于细胞的一侧，直径有5.7μm，呈分裂叶状；与这些细胞邻近的室管膜细胞表面最高处多呈现皱襞。2小时与16小时的实验组的观察结果与对照组相比未发现明显差异。

The distribution of the growth-associated protein (GAP-43) which is close related to the axon regeneration was examined by immunocytochemical method in the adult rat brain and the rat parietal lobus injuried into which the fetal cerebral suspension was lransplanted.

在发现流式细胞计可用于神经科学的研究后，用FCM研究了大鼠脑发育过程中细胞周期的变化和胚胎大脑细胞移植到成年大鼠顶叶后对宿主顶叶脑细胞分裂的影响；由于生长相关蛋白(GAP-43)与轴突再生密切相关，用免疫细胞化学法研究了GAP-43在成年大鼠脑内的分布及顶叶损伤植入胚胎大脑细胞后GAP-43的变化。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

Result: With a high cancer-inhibiting rate on the animal model having been transplanted the cancer, this medicine can remarkably restrain the growth of cancer cells cultivated in vitro and improve the life quality of mouse model, and also shows obvious promotion and enhancement function on the DTH ear-swelling of mouse with cancer, the proliferation of mouse spleen lymphocyte induced by ConA and the cytokine (TNF-α、IFN-γ) of mouse with cancer respectively. It can obviously decrease the adverse effects like weight reduction, damage of viscera and degradation of life quality caused by the chemotherapeutic medicines, and has a remarkable stimulation on the lymphocyte proliferation induced by ConA. It is also able to improve the ratio of SMMC-7721 cells in G0-G1 period and reduce the cell amount in G2/M period to cut down the possibility of stepping into the division period; additionally, it can obviously inhibit some cancer genes of the cancer cells and meanwhile up regulate the cancer-inhibiting gene.

结果：本品能明显抑制体外培养肿瘤细胞的生长，对在体肿瘤移植模型动物具有较高的抑瘤率，能明显提高模型小鼠的生存质量；对荷瘤小鼠DTH耳肿胀、ConA诱导的小鼠脾淋巴细胞增殖、荷瘤小鼠细胞因子（TNF-α、IFN-γ）呈现明显的促进或提高作用；能显著减少化疗药所引起的小鼠体重降低、脏器损害以及生存质量下降等不良作用；本品含药血清对ConA诱导的淋巴细胞增殖具有明显的促进作用；能提高SMMC-7721细胞G0-G1期比例，降低G2/M期细胞数目，而减少其进入分裂期；对肿瘤细胞的多个癌基因具有明显的抑制作用，同时上调抑癌基因等。

The results showed that after 2 h of culture in MEM the chromosomes of oocyte were seperated to form telophase I while a small spindle was observed around chromosomes of primary spermatocyte. However, two clear spindles were observed in the oocytes cultured in CB containing MEM. After further culture, the chromosomes of both primary spermatocyte and oocyte intermingled and formed one large spindle.

在无CB的培养液中培养的卵母细胞培养2小时后，卵母细胞已经进入第一次减数分裂的后期，染色体开始被拉向两极，而精母细胞的MI纺锤体才刚刚形成，虽然继续培养两者染色体可以合二为一并形成一个纺锤体，但是有些染色体发生滞后；当卵母细胞在含有CB的培养液中培养2小时后，在卵母细胞内形成两个相似大小的MI纺锤体，进一步培养形成一个大的纺锤体，染色体正常。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。