细胞分裂

Aim:To observe the amitosis and asymmetric division in subcultured PC12 cells, and to explore the role of amitosis and asymmetric division in tumorigenesis, tumor cell pleomorphism and differentiation.

目的 ：观察传代培养的PC12细胞的无丝分裂和不对称分裂，探讨其在肿瘤发生、肿瘤细胞多形性和细胞分化中的意义。

Moreover, the pattern of odorant receptor gene expression and the organization of odorant receptor genes in cloned mice was indistinguishable from wild-type animals, indicating that irreversible changes to the DNA of olfactory neurons do not accompany receptor gene choice.

过去，克隆的动物一直是用来自分裂细胞的DNA培育的，如皮肤细胞。新的结果表明，来自非分裂的、成熟的细胞的DNA可被重新编排，表达正常发育所需的一套完整的基因。

Puna Chicory, leymus multicaulis and Psathyrostachysjuncea.For the materials used in this article were some old seeds which had been stored for 3-7 years, we found a lot of abnormal mitotic and meiotic cells, such as chromosome bridge. chromosome laggard, micronucleus, multipolar division and so on. Moreover, we also found the wheat"s multi-anther, double germinate-hole"s microspore and collected the photos of every period of meiosis. mitosis.

因为用于细胞学检测的小麦材料是搁置多年的陈旧种子，所以在检测染色体数目时，发现大量异常分裂现象，包括染色体桥、染色体落后、微核、多极分裂等；实验过程中还发现小麦多花药现象和花粉粒具双萌发孔的现象；同时在显微观察同时还注意搜集了细胞有丝分裂、减数分裂各个时期的分裂相图片。

Suppression of Skpi in mitotic HeLa cells by siRNA resulted in accumulation of telophase cells with elongated inter-cell bridges and with midbodies stretched 2-3 times longer than that of normal cells. These Skpi-eliminated or suppressed cells accumulate higher level of CENP-E, suggesting that spatiotemporal regulation of CENP-E degradation at the midbody is essential for cytokinesis.

接下来，我们用RNA干扰技术系统评估了skp1在有丝分裂末期的具体功能，发现缺少了skp1的HeLa细胞无法完成正常的有丝分裂过程，而更多的以四连细胞的形式存在，从而证明了skp1对于正常细胞周期行进所不可缺少的功能。

The head cell would developed into branchy trichomes if the four cells splited into eight ones not in a level,and the one would developed into peltate trichomes if the four cells splited into eight ones in a level.

若四细胞平周分裂成上下八细胞，将发育成分枝状表皮毛的头部；若继续垂周分裂成同层八细胞，将发育成盾状表皮毛的头部。

At time of 5mm length of alabatrum, the miosis in microsporecyte and the sex differentiation was found, thus in female flowers, pollen mother cell showed a abnormal meiose then the pollen cells gradually disintegrated, final, contabescence had taken, and in male flowers, the stamen normally developed.

在幼蕾长到5mm左右，即花粉母细胞开始减数分裂时，出现花性别的分化，雌花中雄蕊花粉母细胞不能进行正常减数分裂，进而花粉细胞逐步解体而导致雄蕊败育；雄花中雄蕊发育正常至性成熟。

The final diagnoses of 12 patients were confirmed aschronic myeloid leukemia. The clinical diagnoses of another 2 pa-tients were acute myeloid leukemia, and the other 1 was secondarypolycythemia. Cytogenetically, the Ph~1 chromosomes were found among 11 of 12 pa-tients with CML, the mean of Ph~1 positive cells was 72.3%. The presenceof P...

在12例慢性粒细胞白血病中，11例Ph~1染色体为阳性(阳性率平均为72.3％)，1例为Ph~1阴性；2例急性粒细胞白血病患者的骨髓中期分裂细胞具Ph~1染色体者分别占1/4及1/5，其中1例还发现微小体；1例继发性红细胞增生症患者，70％的骨髓中期分裂细胞为Ph~1染色体阳性，并发现染色体碎裂细胞。

The final diagnoses of 12 patients were confirmed aschronic myeloid leukemia. The clinical diagnoses of another 2 pa-tients were acute myeloid leukemia, and the other 1 was secondarypolycythemia. Cytogenetically, the Ph~1 chromosomes were found among 11 of 12 pa-tients with CML, the mean of Ph~1 positive cells was 72.3%. The presenceof Ph~1 chromoso...

在12例慢性粒细胞白血病中，11例Ph~1染色体为阳性(阳性率平均为72.3％)，1例为Ph~1阴性；2例急性粒细胞白血病患者的骨髓中期分裂细胞具Ph~1染色体者分别占1/4及1/5，其中1例还发现微小体；1例继发性红细胞增生症患者，70％的骨髓中期分裂细胞为Ph~1染色体阳性，并发现染色体碎裂细胞。

Taxol have been used in clinic therapy of breast cancer. Generally speaking, taxol can accelerate the-assemble of microtubule, stabilize microtubule from its discomposing, make mitosis stagnate at G2/M, sequentially interdict the normal mitosis and proliferation of cells, induce cell apoptosis; Besides, a direct function of taxol on cell signal and gene expression, activations of apoptosis genes and expression of proteins can induce apoptosis.

紫杉醇已应用于乳腺癌的临床治疗，一般认为紫杉醇能增强微管聚合，稳定微管防止解聚，使有丝分裂停滞在G2/M期，从而阻断正常的有丝分裂和细胞增殖；除此之外，紫杉醇可以直接在细胞信号和基因表达上发挥作用，活化多种凋亡相关基因的表达，从而引发细胞凋亡。

Morphology of sexual reproduction abortion in'High Noon', a distant hybridization cultivar of tree peonies(P.delavayi var. lutea×P. suffruticasa), was systematically investigated with light microscopy and cross. The results indicated that:(1)The stamens matured earlier than the gynoecia and pollens dispersed at the early stage of anthesis but the rate of normal 2-celled pollens were merely 2.41%.

采用光镜和杂交等方法对牡丹远缘杂交一代品种'正午'有性生殖败育进行系统形态学观察发现：(1)雄蕊较雌蕊先成熟，初花期即散粉，正常2-细胞花粉仅占2·41%，且花粉在多种培养液中、在自身和其它两种柱头上均不萌发；柱头在谢花期大量分泌粘液，进入最佳授粉状态；(2)同一胚珠中多个大孢子母细胞减数分裂高度不同步，胚囊发育与受精延迟，部分胚乳游离核在细胞化时期分裂异常；(3)自花及品种内异花授粉不结实，天然授粉结实率为0·053‰~0·130‰。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。