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The function of the addition of co-substrate ethanol was to in-situ regenerate NADH. A mathematical model was derived, in which, two substrates were catalytically reacted in sequence. The model was applied to the system involving the reduction of 2'-chloroacetophenone to R-2'-chloro-1-phenyl-ethanol by S.

深入研究了在乙醇存在下生物还原过程中酵母细胞内NADH、NAD〓、NADPH及NADP〓浓度的变化规律,发现胞内NADPH浓度迅速降低到零,而NADH浓度始终维持在较高水平,证明了用于底物还原反应的辅酶是NADH,辅助底物乙醇的加入有利于实现辅酶Ⅰ的原位再生。

The results indicated that the treatment of irradiation restrained the growth and differentiation of protocorm, especially to roots.The LD50 is 67.23Gy, because the protocorm is a integrate embryoids and higher tolerance was found. Ploidy analysis showed that most of aberrant seedlings go with the change of DNA and they were not euploid. When the plants trearted by irradiation, some of aberrant seedlings appeared stem forfication, and leaves appeared chlorophyll-deficient or green-white.,probably due to some genes' expression or closing.

结果表明:辐照处理对种胚原球茎的生长和分化有抑制作用,尤其是对根的抑制更为明显;由于种胚原球茎的完整性好,耐受性强,其辐照半致死剂量(LD50)为67.23Gy;流式细胞分析表明,大多数外部形态发生改变的植株其细胞内DNA的倍性发生了改变,出现相当多的非整倍体;此外,辐照产生的变异苗出现茎分叉、叶片缺绿或白绿相间等现象,可能是由于某些基因的表达与关闭引起的。

Antibody of mouse against rat of glial fibrilla acidic protein used to labeling astrocytes of the brain and antibody of rabbit against rat of c-fos labeling c-fos protein in the brain. The second fluorescence antibodies were FITC and Cy3, respectively. Observations of the results and quantitative analysis were conducted with LSCM.

在本实验中,我们应用免疫组织化学的双标方法,用小鼠抗大鼠胶质原纤维酸性蛋白标记大鼠脑内的星形胶质细胞,用兔抗大鼠c-fos标记大鼠脑内的c-fos蛋白,分别应用FITC和Cy3标记的荧光二抗来显示,最后用激光扫描共聚焦显微镜进行观察并定量分析。

While 30μmol·〓 FRCRSFa and 10μmol·〓 E-4031 were dialysed intracellularly via the patch-pipette, FRCRSFa significantly decreased 〓 exchange current and there was no the further decrease after adding FRCRSFa in the perfused solution with the same concentration, whereas E-4031 dialysed had no effect on 〓 exchange current, but a obvious increase of it occurred when adding E-4031 to the perfused solusion with the same concentration.

分别将30μmol·〓的FRCRSFa和10μmol·〓的E-4031加入微电极内液中,FRCRSFa显著抑制了〓交换电流,当在细胞外灌流液中加入同样浓度的FRCRSFa时无进一步抑制;而胞浆内的E-4031对〓交换电流无显著作用,而在灌流液中加入同样浓度的E-4031后,却显著增强了〓交换电流。

Newborn Balb/c mice were infected intracranially with CVB3 (10〓). Indirect immunofluorescent staining approved the infection. We monitored daily for overt signs of infection and pathological changes (nervous cell necrosis, edema, etc). The most severe signs and pathologic changes happened at the third day postinfection.

本实验又选用日龄为2-3天的新生Balb/c小鼠,以10〓CVB3标准株颅内接种,逐日观察CVB3引致小鼠脑组织感染的病理改变及症状一周,并以间接免疫荧光法证实小鼠脑内感染确立,结果显示:10〓CVB3导致小鼠颅内感染成立;小鼠出现明显的症状体征;组织学呈现明显的神经细胞变性、坏死及细胞内、外严重水肿,且以第三天为最重。

Note the very large cells that have large violet intranuclear inclusions with a small clear halo. Basophilic stippling can be seen in the cytoplasm.

在这些大的细胞内可大的兰紫色的细胞核和小的透明的圆晕,并细胞质内可见噬碱性颗粒。

Results The choroid plexus, hypophysis, area postrema and pineal body, namely, circumventricular organs and ependyma as well as pia mater layers were stained by the HRP reaction products.The HRP reaction products were observed in the tissue space of membrane mucosa nasi, around the fila olfactoria and venules, and within the venules under the light microscopy and electron microscopy, also. Scintillation count showed that the radioactivity of the CVOs was higher than that of the background. The radioautography showed that the scattered grains of silver existed within the cells of neurocyte layer also.

结果 在脑室周围器官及脑室周围室管膜、软脑膜层有HRP反应产物的聚集,在鼻中隔的鼻粘膜下组织间隙、嗅丝周围间隙及静脉周围和管腔内也可见到HRP反应产物;液闪计数显示,大白鼠的脉络丛、脑垂体、最后区、松果体等脑室周围器官放射性活度明显高于本底;放射自显影显示大白鼠脑组织神经层细胞内有散在分布的银颗粒。

We innovate that optical tweezer technology can be used in microchannel. At last, yeast cell was trapped and manipulated by the focused laser beam in the microchannel we machininged.

文中创新性的提出光镊技术在微米通道内的应用,并且成功地在激光加工的石英微米通道内实现酵母细胞的捕获与操纵。

In order to understand antioxidant enzymes activities in response to temperature increment and the active oxygen free radical clearing function of antioxidant enzymes in antarctic ice mircoralgae Chlorophyceae L4, the free radical generation rate, the malonaldehyde content changes and the corresponding antioxidant enzymes activities changes in Chlorophyceae L4 at different temperatures were determined.

为了阐明南极冰藻Chlorophyceae L4抗氧化酶活性对温度升高的响应以及了解冰藻细胞内抗氧化酶系统对活性氧自由基的清除作用,对不同温度下(包括最适温度6℃、高于最适温度10℃和15℃)Chlorophyceae L4细胞中自由基的产生速率、丙二醛含量的变化和相应的抗氧化酶系统活性的变化进行了测定。

Phosphorylation and dephosphorylation of proteins are a ubiquitous and covalent modification in the living cells, and they are almost involved in all physiological and pathological processes, e.g., metabolisms, growth, development, carcinogenesis.

蛋白质的磷酸化与去磷酸化是细胞内普遍存在的一种共价修饰方式,它几乎涉及所有的生理及病理过程,如代谢、细胞的生长发育、癌变等。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?