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In addition, baculovirus are noninfectious to vertebrates, and their promoters have been shown to be inactive in mammalian cells. Futhermore, the expression level of some recombinant proteins is up to 1000 mg/L. Finally, recombinant proteins are soluble and antigenically, immunogenically and functionally similar to their authentic counterparts. In this article, we used the transfer vector pBlueBac of AcNPV (Autographa californica Nuclear Polyhedrosis Virus) to construct the recombinant baculovirus AcNPV-GM-CSF by in vivo recombinantion.

本文中,我们首先利用苜蓿夜蛾核型多角体病毒带β-Galactosidase 基因标记的非融合蛋白基因转移载体pBlueBac,通过细胞内同源重组的方式将hGM-CSF基因成功地插入病毒AcNPV的基因组中。hGM-CSF基因编码信号肽和完整的氨基酸序列。hGM-CSF基因在感染重组病毒的草地夜蛾培养细胞Sf9中得到表达,感染后的Sf9细胞培养液能刺激人骨髓细胞在体外形成典型的集落,表达水平可达2.7〓CFU/ml。

Microtubules and their textural composite hydrate system, nuclear envelope inside the cell, the places of plasma membrane infolding and cell junction and cell adhesion outside the cell possess lots of nano-scale (100-103 nm) narrow spaces.

在细胞内部的微管及其织构复合水化体系、核膜以及在细胞外部的质膜内褶和细胞连接粘连之处都可以发现许多纳米尺度(100~103 nm)缝隙空间。

The expression of VEGF was observed by using image analysis and the secretion of VEGF by macrophagus was tested by using immunohistochemistry method.

结果:在口腔鳞癌中,VEGF的表达和微血管计数、巨噬细胞计数呈现正相关(P.05);VEGF的表达、巨噬细胞的浸润和肿瘤的淋巴结转移有关(P.05),口腔癌浸润的巨噬细胞中有29%~10%的细胞内有VEGF的表达。

In the present study, the effects of chloride channels blockers,(Niflumic acid,NFA;IAA-94;DIDS) on EJP were explored in guinea pigs, using intracellular recording techniques on acute isolated spiral modiolar artery.

本实验运用细胞内微电极记录技术,在豚鼠耳蜗螺旋动脉(spiral modiolar artery, SMA)离体标本上,研究氯通道阻断剂(Niflumic acid, NFA; IAA-94; DIDS )对去甲肾上腺素引起SMA平滑肌细胞的反应和平滑肌细胞EJP的影响。

In one type of AML, called acute monocytic leukemia, the leukemia cells are more likely to invade the lining of the spinal canal or brain.

与急性髓细胞白血病的其他亚型相比,急性单核细胞白血病患者的肿瘤细胞更容易侵入椎管或脑内。

Application of SOD and /or Hypoxia-preconditioning can prevent myocardiac injury partly by reducing the myocyte apoptosis.

SOD和或缺氧预处理具有抗心肌细胞损伤和减轻心肌细胞凋亡的作用,而且可能是通过降低细胞内钙离子浓度而起作用的。

In normal myocardium, sorcin extensively co-localized with ryanodine receptors, whereas in myopathic hearts, the degree of co-localization was markedly disrupted.

正常心肌细胞中,sorcin与细胞内钙释放通道RyR能广泛协同定位,而在心肌病的心肌细胞中,这种协同定位的能力被破坏。

In general, osteoporosis is a disease of bone which is caused by an imbalance between osteoblastic bone formation and osteoclastic bone resorption. However, the recent research showed that the ability of bone marrow mesenchymal stem cells differentiates into adipocytes is greater than osteoblasts and it was thought to cause into osteoporosis, such as diabetes.

骨质疏松症一般被认为是细胞的骨生成作用与破骨细胞的骨吸收作用失去平衡,然而近年来的研究指出骨头内的间质干细胞分化成造骨细胞的能力减弱或是间质干细胞分化成脂肪细胞的能力增强,这样的结果往往会导致骨质疏松症,如糖尿病状态。

In general, osteoporosis is a disease of bone which is caused by an imbalance between osteoblastic bone formation and osteoclastic bone resorption. However, the recent research showed that the ability of bone marrow mesenchymal stem cells differentiates into adipocytes is greater than osteoblasts and it was thought to cause into osteoporosis, such as diabetes.

中文摘要骨质疏松症一般被认为是细胞的骨生成作用与破骨细胞的骨吸收作用失去平衡,然而近年来的研究指出骨头内的间质干细胞分化成造骨细胞的能力减弱或是间质干细胞分化成脂肪细胞的能力增强,这样的结果往往会导致骨质疏松症,如糖尿病状态。

THe cell feature in upper and anterior AV node, His bundle and right branch is between Purkinje and myocardial cell. Typical P cells can not be seen.

结上部和前部、房室束、右束支内的细胞在形态上介于Purkinje细胞和心肌之间,未见典型的P细胞。

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Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

However, to get a true quote, you will need to provide detailed personal and financial information.

然而,要让一个真正的引用,你需要提供详细的个人和财务信息。