细胞内的

Results AQP1 is expressed at the apical and basolateral membrane of the microvascular endothelium; AQP3 was detected at basal cells of both the bronchiole epithelium and submucosal gland acinus; AQP4 is present in the basolateral membrane of columnar cells in bronchiole; while AQP5 is expressed in the apical membrane of type Ⅰ pneumocytes, and also at the apical of columnar cells of superficial epithelium and submucosal gland acinar cells.

结果本研究发现AQPs基因在羊肺中的表达分布与人相似，AQP1在肺内的毛细血管内皮细胞表达；AQP3在小支气管黏膜上皮的基底细胞的基侧膜表达，AQP4存在于小支气管黏膜上皮的柱状纤毛细胞的基侧膜；AQP5存在于Ⅰ型肺泡上皮细胞的顶质膜，存在于小支气管黏膜上皮柱状纤毛细胞，以及在气道黏膜下腺的腺细胞的顶质膜表达。

Calcium antimonite deposits, which indicates calcium distribution, were mainly localized in cellmembrane and vacuoles, as a storeroom of Ca2+, and smaller amounts of calcium p recip itates randomly resided in mitochon2dria, cytop lasm and nucleus.

结果表明，对照生长条件下(14 mmol·L-1 NO3-)，黄瓜根系分生区细胞内Ca2+主要出现在细胞膜和小液泡内，线粒体、细胞质和细胞核及核仁内也有少量的较小颗粒钙沉淀。

Research Direction Our research try to block the activation of transmembrane signal transduction related to inflammation by blocking the receptor of cellular membrane.

研究方向细胞内信号转导通路的激活要通过细胞膜受体的介导，本研究拟阻断细胞膜受体的激活，从而阻断肺细胞内各种炎症相关性信号转导通路的激活。

Methods:(1) Fura-2 based calcium imaging technique was used to examinate the effects of leptin on the basal [Ca2+]i, and the modulation effects of leptin on KCl-evoked [Ca2+]i increase in long-term cultured hypothalamic neurons.

对四个培养时间段的下丘脑神经元分别进行以下实验：(1)采用荧光钙离子成像技术，研究瘦素对细胞内静息钙离子浓度的影响，及其对25 mM KCl引起的细胞内钙升高的调节作用。

Poptosis-inducing factor is a mitochondrion-localized flavoprotein with NADH oxidoreductase activity, which is encoded by the nuclear gene. Following induction of apoptosis, AIF is released from mitochondria and translocated to cytosol, and then to the nucleus. The mechanism of AIF translocation from mitochondria to nucleus is still unclear. It has been suggested that translocation is due to the presence of nuclear localization signal.

bstract： AIF(apoptosis-inducing factor)为位在粒线体内膜膜上之蛋白质，在正常细胞中，能够藉由维持电子传递链复合体I的稳定性，进而影响细胞能量的产生及自由基的清除，并且也会维持粒线体的构造；反之，当细胞遭受到不当刺激时，粒线体膜通透性改变，使得原本存在於粒腺体间质中之AIF，被释放出来，进入细胞核内造成DNA片段化，进一步促进细胞凋亡。

FRAP is a technique widely usedin cell biology to observe the dynamics of biological systems, including the diffusionof membrane components.

FRAP是在细胞生物学中被广泛用于测定生物体系动态性包括生物膜组分动态扩散的一种技术，其原理是利用高强度激光将细胞内一小部分区域的荧光分子漂白，然后通过记录该区域随后的荧光恢复，检测周围未漂白区域内的荧光分子与漂白区域荧光分子的交互扩散。

RESULTS: We found that (1) tramadol, similar to the well-knownTRPV1 agonist, capsaicin, significantly increased [Ca2+] i ofTRPV1-CHO cells in a concentration-dependent fashion;(2) itseffect was reversibly prevented by the TRPV1 antagonist capsazepine;(3) repeated application of tramadol resulted in marked tachyphylaxis;and (4) tramadol did not modify [Ca2+] i in control CHO cells.

结果：我们发现以下结果：（1）曲马多和辣椒素这个熟知的辣椒素受体激动剂一样，呈浓度依赖性地显著增加 CHO 细胞 TRPV1内流钙离子；（2）该作用可以被 TRPV1拮抗剂 capsazepine 可逆性地阻止；（3）重复给予曲马多会造成显著的快速耐药性；（4）曲马多不修饰钙对照 CHO 细胞中的内流钙离子。

We synchronized the wheat cell suspension cultures, then added Novobiocin—an inhibitor of TopoⅡ to the cell suspension, changs of the ratio of M/T were observed within 48 hours.

用aphidicolin使小麦悬浮系细胞同步化，然后向细胞培养液中加入TopoⅡ抑制剂新生霉素，48小时内观察分裂中期细胞比率的动态变化。

In the present study, CHL cells were cultured routinely in MEM medium for 3 days. Then the cells were treated with sham exposure , 0.4mT 50 Hz sinusoidal MF , 0.4mT noise MF and the combined noise and 50Hz MF for 3 min and 15min, respectively. After exposure, the cells were lysed, and the proteins were extracted.

实验中，将中国仓鼠肺成纤维细胞分别经0.4mT工频磁场、等强度的噪声磁场、工频磁场与噪声磁场的复合磁场辐照及假辐照处理3min和15min后，裂解细胞，提取细胞蛋白质，以Western印迹技术，测定并比较细胞内SAPK磷酸化的变化。

The results showed that after 2 h of culture in MEM the chromosomes of oocyte were seperated to form telophase I while a small spindle was observed around chromosomes of primary spermatocyte. However, two clear spindles were observed in the oocytes cultured in CB containing MEM. After further culture, the chromosomes of both primary spermatocyte and oocyte intermingled and formed one large spindle.

在无CB的培养液中培养的卵母细胞培养2小时后，卵母细胞已经进入第一次减数分裂的后期，染色体开始被拉向两极，而精母细胞的MI纺锤体才刚刚形成，虽然继续培养两者染色体可以合二为一并形成一个纺锤体，但是有些染色体发生滞后；当卵母细胞在含有CB的培养液中培养2小时后，在卵母细胞内形成两个相似大小的MI纺锤体，进一步培养形成一个大的纺锤体，染色体正常。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷