细胞内的

FCM analysis showed that the apoptotic rate of MDA-MB-231 cells and intracellular Ca(superscript 2+) concentration increased gradually with the increase of deguelin concentration. RT-PCR and Western blotting analysis showed that the expression of Bcl-2 mRNA and protein was clown-regulated and that of Sax was op-regulated after deguelin treatment.

流式细胞术检测显示细胞凋亡率和细胞内钙离于浓度随鱼藤素浓度的增加而增多；RT-PCR和蛋白印迹检测结果发现鱼藤素能使Bcl-2 mRNA和蛋白表达减少，而Bax表达增加。

A total of 90 healthy male Sprague Dawley rats were selected for this study. Twelve rats were used for the sham operation group. Left anterior descending coronary artery of the remaining rats was deligated to establish models of myocardial infarction. After 24 hours of deligation, 36 rats were randomly divided into the control group and experimental group, 18 rat in each group.

目的：检测人胰岛素样生长因子Ⅰ基因修饰的鼠骨骼肌成肌细胞移植后，心肌梗死大鼠血浆及心肌组织内胰岛素样生长因子Ⅰ蛋白浓度的变化，以及心肌细胞内胰岛素样生长因子Ⅰ和端粒酶反转录酶mRNA水平的表达。

Consequence:Afer duplicating the model,many patho-representations occur in theaging rats including the worse general states,increasing active oxygen freeradical,lipid peroxidation and apoptosis,reducing antioxygen capability andionic transport capability in the cell envelope,overexpression of P16,impairing hippocamp nerve cell architecture and depositive lipofuscin inultramicro-morphology.

结果：D-半乳糖致亚急性衰老大鼠出现一般状态较差、活性氧自由基和脂质过氧化产物增加、抗氧化能力明显下降、细胞膜离子转运能力降低、P16过度表达、细胞凋亡增加、海马神经元细胞超微结构损伤以及神经元细胞内脂褐素沉积的病理表现。

Among the target tissues of estrogen is endometrium, which undergoes cyclic proliferation, differentiation and desquamation.

目前认为，细胞内E2的浓度不仅决定于内分泌的合成，而且主要受靶细胞内E2合成和代谢的影响。

Mucous membrane protective screen is formed by the join between cell of alvine mucous membrane and cell, atrophy of cell of alvine mucous membrane, wither dies, cellular connective can send alvine path to connect flabbily appear a gender to increase, provide passageway; chemical protective screen for the bacterium inside bowel and toxin the digestive juice that shows epithelial excretive mucus and alvine memory are in bowel and digest enzymatic the chemistry that waits for material to reach its to develop.

黏膜屏障由肠黏膜细胞和细胞间的连接构成，肠黏膜细胞萎缩、凋亡、细胞连接的松弛可致肠道通透性增加，为肠内细菌及毒素提供通道；化学屏障指肠上皮细胞分泌的黏液及肠内存在的消化液和消化酶等物质及其发挥的化学功能。

Las multiplied again in the hemocoel and then invaded other host tissues, including hemocytes, nervous tissue, fat body, trachea, and muscle. Eventually, Las invaded the salivary gland and the Las-infected psyllids were enabled to transmit Las to other host plants. Symptoms of serious pathogenesis occurred in the muscle tissue.

黄龙病菌必需先穿过中肠上皮细胞并於中肠细胞内进行增殖，接著进入血腔继续进行增殖，并逐渐移行至其他组织包括血球、神经、脂肪体、气管上皮细胞及肌肉等，最后抵达唾腺，届时柑橘木虱即具有传播黄龙病的能力。

PartⅡThe mechanism elucidation about effects ofα-(2,3)/(2,6)sialic acid on the Cx43gap-junction functions.(1) Westernblotting experiment showed that the decrease of sialic acid didn\'t changethe Cx43 expression and its phospholation level.(2) Westernblotting experiment showed sialidase didn,t change the ZO-1 expression,IP and confocal experiment showed sialidase improved the interaction of Cx43 andZO-1.(3) Westernblotting experiment showed sialidase didn\'t change N-cadherin expression,IP and confocal experiment showed sialidase promoted the complex formation ofCx43 and N-Cadherin.(4)Sialidase could increase the ERK1/2 phospholation level,and enchanedintercellular homotypic adhesion,Immunofluorometric assay showed sialidase couldpromote the N-cadherin cluster on the membrance.

第二部分：α—(2，3)／(2，6)唾液酸对肿瘤细胞CX43间隙连接功能影响的机制研究1、Westernblotting结果表明降低细胞膜表面唾液酸并不改变Cx43的表达及其磷酸化水平：但Cx43连接斑形成增多；2、Westernblotting结果表明唾液酸酶作用后细胞ZO—1表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与ZO-1的结合；3、Westernblotting结果表明唾液酸酶作用后细胞N-cadherin表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与N-Cadherin复合物的形成；4、唾液酸酶作用后细胞内ERK1／2磷酸化水平明显增加，细胞间同质粘附增加，以及免疫荧光表明唾液酸降低后可促进N-cadherin的膜成簇。

Objective To study the feasibility of direct intramyocardial injection of exogenous bFGF and BDNF microspheres to the cardiac myocytes.

第一部分 bFGF和BDNF缓释微粒的构建、组织相容性及心肌细胞内表达的研究目的：探讨外源性bFGF和BDNF缓释微粒在心肌细胞内表达的可行性。

Flow cytometry was used to detect intracellular c-FLIP protein in peripheral T and B lymphocytes, immunohistochemistry to examine the expression of c-FLIP in lesional tissue.

采用流式细胞仪检测30例寻常性银屑病患者和20例正常人对照组外周血T细胞和B细胞内c-FLIP蛋白表达阳性率，采用免疫组化方法检测c-FLIP蛋白在其皮损中的表达。

METHODS:①The microdose cytopathogenic effect inhibition assay was used in Hep 2 cell culture to investigate the way through which QiqinⅠinhibited the virus.

①在Hep 2细胞上采用微量细胞病变抑制法，从直接灭活病毒、抑制细胞内病毒复制、阻断病毒吸附三方面研究芪芩Ⅰ号对CVB3的抑制作用。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷