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The possible mechanism of phycocyanin in the proliferation inhibition of K562 cells is to increase the expression of FAK gene. The phcocyanin may be considered as a potential agent for inhibition of cancer cell proliferation.

藻蓝蛋白可能通过增强细胞基质与K562细胞表面的整合蛋白β1结合,上调K562细胞内FAK基因表达水平,恢复整合蛋白β1介导的细胞增殖抑制信号而发挥抑制K562细胞增殖的作用。

Adenovirus 5第二军医大学博士学位论文英文摘要胸心外科专业(WAd5) and PDC-MLC-VEGF were transferred to the adenoviral packaging cell HEK 293cell by lipofectamine 2000 mediated gene transfer method to pack the virus; The desired Advectors were purified by density gradient ultracentrifuge and titrated in 293 cells afteridentified.

Ad.mlc-hVEGF165 体外转染心肌细胞的实验研究分离培养乳鼠心肌细胞,在同一 MOI 值下,Ad.mlc-hVEGF165 和 Ad.hVEGF165分别转染心肌细胞,以 RT-PCR 法和 Western 印迹分析法分别检测转染后心肌细胞内VEGF mRNA 的转录及 VEGF 蛋白的表达,以 ELISA 法检测转染后心肌细胞分泌的VEGF 蛋白质,MTT 等方法观察其对促内皮细胞增殖作用。

Hyalinocytes can phagocytize the granulocytes which have expelled their granules. In hyalinocytes, ACP was found synthesized in Golgi apparatus and was involved in the endocellular digestion process.

透明细胞可以吞噬排放颗粒后的颗粒细胞,透明细胞中由高尔基体合成的酸性磷酸酶等溶酶体酶主要用于透明细胞的细胞内消化作用。

The data shoewed that esculetin, resveratrol enhanced LPL expression up to about 1.2 ~3.5 fold compared with the control group. In addition to fibrates, however, no effect on LPL expression by curcumin was observed. We concluded that esculetin and resveratrol enhanced LPL expression on CHO-K1 cell. We hope that we can apply these drugs to mutant form LPL, furthermore apply theses drugs into clinical treatment.

利用药物处理有转染带有完整人类脂蛋白解脂酶启动子和脂蛋白解脂酶cDNA 序列(-1715 ~+1643)质体的CHO-K1细胞,从细胞培养液以及细胞溶解物中发现,除了curcumin之外所有药物都可以使得细胞培养液以及细胞溶解液内的脂蛋白解脂酶活性有1.2至3.5倍的上升,由此结果可知除了curcumin外,esculetin和白藜芦醇以及其他fibrate药物可降低三酸甘油脂血症。

There were five groups in the examination of cellular levelK562 group,K562/A02 group,K562+ADM group,K562/A02+ADM group and K562/A02+TTD+ADM group).The non-cytotoxicity doses to cell lines K562 and K562/A02 of TTD were got by MTT assay.Using flow cytometry (FCM assay to examine the intracellular ADM concentration.There were three groups in the examination of genic,zymologic and protein levelsK562 group,K562/A02 group and K562/A02+TTD group).The mRNA expression of MDR was measured by fluorescent quantitative reverse transcriptase polymerase chain reaction(RT-PCR.The expression levels of glutathione-S-transferase and topoisomerase Ⅱ were determined by immunohistochemical technique.

细胞水平检测实验分5组(K562组、K562/A02组、K562+ADM组、K562/A02+ADM组和K562/A02+TTD+ADM组),采用MTT法检测TTD对K562和K562/A02细胞的非细胞毒性剂量,流式细胞术检测细胞内阿霉素的浓度,基因、酶学、蛋白水平检测实验分3组(K562组、K562/A02组和K562/A02+TTD组),采用RT-PCR法检测mdr1 mRNA的表达,免疫细胞化学方法检测谷胱甘肽S转移酶π和拓扑异构酶Ⅱ的表达水平,Western-blotting法检测P-糖蛋白和bcl-2表达。

The former formed a kind of KR cyst, while the later formed a kind of NKR cyst. All the kinetosomes, ciliary shafts and microtubules were resorbed in the former, meanwhile, the cell lost most water and cell volume decreased distinctly. In the later, only part of kinetosomes and part of microtubules were resorbed. Cell lost water slightly and volume decreased slightly.

期间,细胞吸收了全部的毛基体、纤毛杆和微管结构,伴随着细胞剧烈的失水浓缩,细胞体积显著缩小;游仆虫在形成毛基体非吸收型包囊时,细胞仅经历了部分的脱分化过程,期间,细胞仅吸收了部分的毛基体及部分微管结构,并且细胞仅发生了轻微的脱水和浓缩,细胞以折叠的形式容纳在包囊壁内。

Methods: After LNCaP and PC3 cell lines were affected by 10, 25, 50, 75, 100 μmol/L curcumine respectively, the cell activity was assayed with MTT method at 5, 12 and 24 hours respectively; Electronic microscopy and flow cytometry were adopted to observe the morphological changes and the cell cycle of LNCaP and PC3 cell lines at 24 hours.

分别用10、25、50、75、100μmol/L浓度的姜黄素对LNCaP及PC3前列腺癌细胞株进行体外干预,5、12、24 h后四氮甲唑蓝比色法检测细胞生长活性;24 h后透射电镜观察细胞超微结构变化,流式细胞仪测定细胞凋亡及细胞周期的变化;5 h后Western印迹法检测细胞内IκBa的表达。

Micro-tube lysosome with ACPase positive was firstly found since 1973, this kind of structure was observed in many cells, and called line lysosome, these two kinds of lysosome were regarded as manifestation pattern in different period, and was round when it was in relative silence, and line when activating state.

微管是细胞骨架中最粗的一种纤维丝,在活细胞内微管是一种动态的结构,在一定条件下可聚合或解聚,因此细胞内呈现了各种形态的排列方式,以适应变动的细胞质和完成它们各自的功能。

In human vascular smoothe muscle cells and glial cell tumors, ADM suppressed cell growth and increased intracellular cAMP.

对于人的血管平滑肌细胞和胶质细胞,ADM抑制其细胞增殖并增加细胞内cAMP水平。

F-actin can express in all kinds of cells of Corti organ, mostly in inner pillar cells and outer pillar cells. They were light red fluorescence.

1。正常成年大鼠耳蜗Corti器F-actin在各类细胞内均有表达,内、外柱细胞着色较强,呈现明亮的红色荧光。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是,在否定句或疑问句里,这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷

Nusa lembongan : Bali's sister island, coral and sand beaches, crystal clear water, surfing.

Nusa Dua :豪华度假村,冲浪和潜水,沙滩,水晶般晶莹剔透的水,网络冲浪。