细胞内的

Active oxygen is found to be involved in apoptotic progress affected by exogenous SOD and measurement of SOD in cell sap. Arsenic compounds cause a decrease of SOD in cell. It is exactly the decrease that make plenty of active oxygen gather in mitochondria, and starts the apoptotic progress. The analysis of SOD values shows that active oxygen has an effect on apoptosis.

通过加入外源性SOD和对胞液SOD的测量，发现胂化物引起细胞凋亡的过程，有活性氧的参与，并且胂化物还导致细胞内SOD值的减少，正是由于这种降低，才导致了细胞内特别是线粒体中活性氧大量聚集，启动了细胞凋亡过程，通过对SOD值的分析，证明了活性氧在细胞凋亡中的作用。

Results: electronic microscope showed that in prednisone group heterochromatin increased in some cells, entoblast was not obvious in some cells, fat drops were small and few, mitochondria vacuole were common, and myelin figure was found in smooth endoplamic reticulum. the ultrastructure in yc group was almost normal and megamitochondrion formation was common. a large amount of fat drops were found in cytoplasm in gw group and little improvement was found compared with that in prednisone group.

结果： 电镜结果显示：激素组个别细胞异染色质增多，核仁不明显，脂滴显著变小变少，线粒体空泡化常见，有滑面内质网髓鞘样结构形成；养阴组电镜结构基本正常，其特征是细胞内有较多的巨大线粒体形成；桂附组较激素组改善不明显，其特征是胞质内可见大量脂滴。

We also have observed some organs pathological changes of juvenile Jian Carp, such as pancreas acinous gland and zymogen granules of acinous gland cell disappearing, intercellular substance hyperplasia and inflammatory cells soakage, hepatatrophia, liver cell granular or vacuolar degeneration and necrosis, karyolysis or pyknosis, glycogen granules decreasing, metanephros atrophy, metanephric canaliculus epithelium granular or vacuolar degeneration and necrosis, mitochondrion swelling and mitochondrion cristae disappearing, karyolysis, distal convolutal tubule microvilli desquamating, spleen marrow cell degeneration and necrosis, intercellular substance of spleen hyperplasia, spleen atresia, blood corpuscle disappearing.

后肾土黄色、淡褐色或苍白色，肾小管上皮细胞肿胀、颗粒或水泡变性、坏死，细胞内有大量血细胞流出，线粒体肿胀，嵴结构消失，细胞核溶解，肾间质甲状腺滤泡和拟淋巴细胞增生，远曲小管微绒毛脱落、管道细胞界限不清。心脏肌纤维肿胀、颗粒或空泡变性，严重的肌纤维溶解、变细或断裂，肌纤维间水肿、炎性细胞浸润，部分心肌细胞核浓缩。脾脏暗褐色，脾髓质细胞变性、坏死，拟淋巴细胞明显减少，淋巴细胞岛少见、岛中细胞成份减少，黑素巨噬细胞中心减少、体积缩小，脾脏网状基质水肿，脾窦闭锁，血细胞减少。

Results:(1)With the increase of SPIO concentration,the number of intracytoplasmic particles stained with Prussian Blue stain and the mean cellular iron content increased accordingly.

结果：(1)随着SPIO浓度的增加，MSCs细胞内平均含铁量相应升高；细胞涂片普鲁士蓝染色也显示细胞内蓝染颗粒相应增加。

Incubation of neutrophils with these components for 30 minutes did not affect the production of intracellular ROS. ANE (1.56 microgram/ml), arecoline (0.016 mg/ml), safrole (1.25 mM ) or eugenol (1 mM ) decreased approximately 50% of the intracellular ROS production of neutrophils that were treated with CB/ fMLP .

此外，以槟榔嚼块内所含成分分别处理嗜中性白血球30分钟，并不影响细胞内活性氧化物产生，然而约在 ANE (1.56 microgram/ml)、 arecoline (0.016 mg/ml)、 safrole (1.25 mM )或 eugenol (1 mM )浓度下，嗜中性白血球被 CB/ fMLP 活化而细胞内产生活性氧化物的能力则下降50 %。

Results: in contrast with model group, SSM can (1) increase the survival rate of ARF in rats, cut down Scr and BUN at 24hr, improve renal function, slightly surpass Vp in its therapeutic effect;(2) mitigate the extent of renal pathologicchanges, decrease the numbers of renal tubule necroses and casts;(3) protect renal ultrastructure such as microvilli, mitochondria, endoplasmic reticulum of TEC, podocytic process of glomerulus epithelial cell,endothelial cell etc, better than Vp;(4) enhance SOD activity, lower MDA contents in renal cortex;(5) enhance NO contents in serum, reduce ET levels in plasma;(6) evidently cut down TNF- a contents in serum, superior to Vp;(7) antagonize calcium overload in TEC;(8) alleviate renal pathological changes in ARF metaphase (at 3d), advance regeneration and recovery of TEC, restore renal structure in ARF convalescence (at 5d), and better than Vp;(9) increase the expression of TEC PCNA and prepro EGFmRNA in ARF metaphase.

结果：与模型组相比，SSM能(1)提高ARF大鼠的存活率，降低24hr时Scr及BUN，改善肾功能，其疗效略优于维拉帕米；(2)减轻ARF肾脏病变程度，减少24hr肾小管坏死数及管腔内管型数；(3)对肾脏的超微结构如肾小管上皮细胞微绒毛、线粒体、内质网、肾小球上皮细胞足突、内皮细胞等形态结构均有一定的保护作用，其疗效略优于维拉帕米；(4)提高肾皮质SOD活性，降低MDA含量；(5)提高血清NO水平，同时降低血浆ET含量；(6)显著降低血清TNF-α的水平，疗效明显优于维拉帕米；(7)拮抗小管上皮细胞[Ca~(2+)]_i的升高；(8)使ARF中期(第3d时)肾组织病变较同期其它两组明显减轻，并使肾小管细胞再生修复提前，至ARF恢复期(第5d时)肾组织结构恢复重建良好；作用明显优于维拉帕米；(9)促进ARF中期肾小管上皮细胞PCNA的表达及EGF前体mRNA的合成，从而增加EGF的合成释放，而维拉帕米无此作用。

Results:（1）After the action of electrets, 10%of 3T3 cells appeared apoptosis.(2) After the action of electrets in 3T3 cells, the free Ca2+ levels in cellular plasm were increased. The increasement of Ca2+ levels was caused by the Ca2+ inflow from outside cell, rather than the release of calcium pool inside cell.

结果：(1)负极性驻极体作用3T3细胞后，3T3细胞出现10％的凋亡量；(2)驻极体作用3T3细胞以后，细胞质游离Ca2+浓度升高，Ca2+浓度的升高不是由于细胞内钙池的释放，而是由于细胞外Ca2+内流所引起。

Methods Explore the optimal concentration of phurbol-12-myristate-acetate and calcium ionophore (A23817) inducing time, and optimal concentration of biotinyl sheep anti mouce IgG (bio-SAM-IgG) and horse radish peroxidasestreptavidin. The CD4(superscript +) cell enriched from peripheral blood mononuclear cells using solid phase anti-CDs monoclonal antibodies were induced by PMA and A23817 under influence of monensin and harvested in vary hours.

从健康人外周血单个核细胞中富集CD4细胞，加入莫能霉素，用不同浓度的佛波醇酯和钙离子载体(A23817)诱导CD4细胞不同时间，比较其胞内表达白细胞介素－2(IL-2)/白细胞介素－4(IL-4)的细胞百分率，确定最适诱生条件；用不同稀释度的IL-2McAb、IL-4McAb、不同浓度的生物素化羊抗鼠IgG(bio-SAM IgG)和辣根过氧化物酶标记的链霉亲和素作棋盘配比试验，确认McAb、bio-SAM IgG和HRP-SA的最适浓度，根据上述结果建立了检测细胞内细胞因子的BSA-ICC法。

The fulllength heparanase gene is isolated. The functional heparanase is expressed within COS7 and CHO cells and few heparanse is secreted into medium. The positive CHO cells expressing active heparanase is screened, which will help explore its function and screen its antibody and antagonists from a variety of sources.

本研究分离出人HPA编码基因全序列；在真核细胞内成功表达出有活性的人HPA，发现此酶在COS7和CHO细胞中表达在细胞内，很少分泌到细胞外；筛选出其恒定表达细胞株，为进一步研究HPA结构功能、开发其抗体及抑制剂提供了有力的工具。

The fulllength heparanase gene is isolated. The functional heparanase is expressed within COS7 and CHO cells and few heparanse is secreted into medium. The positive CHO cells expressing active heparanase is screened, which will help explore its function and screen its antibody and antagonists from a variety of sources.

本分离出人HPA编码基因全序列；在真核细胞内成功表达出有活性的人HPA，发现此酶在COS7和CHO细胞中表达在细胞内，很少分泌到细胞外；筛选出其恒定表达细胞株，为进一步研究HPA结构功能、开发其抗体及抑制剂提供了有力的工具。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。