细胞内的

The results demonstrated that the intracellular pH change was different when the Hela cells was treated with different anti-tumor drugs, some would caused intracellular acidification, some would caused ntracellular basification, and some would barely caused pH change. The single living Hela cells uptaken with the ratiometric pH nanosensor was also imaged with laser confocal microscope, which were treated with anti-tumor drugs.

通过利用该纳米传感器对硫酸长春新碱诱导后的Hela细胞内pH变化的活体、原位、实时监测，发现在硫酸长春新碱诱导引起凋亡的Hela细胞中，细胞内的pH值由诱导前的7.11酸化为6.51，并且在一定的浓度和时间范围内，硫酸长春新碱诱导后细胞内的酸化率与诱导药物的浓度和诱导时间成正相关关系。

It is widely accepted that AP depolarize the plasma membrane and open voltage-dependent calcium channels , the calcium influx through VDCCs trigger the fusion of vesicles with plasma membrane and subsequent transmitter release.

现在被普遍接受的观点是当可兴奋细胞受到动作电位的刺激后，细胞的电压依赖性钙离子通道被打开，在动作电位的下降相引起细胞外钙离子的内流从而升高细胞内的游离钙离子浓度，触发许多细胞内的过程，譬如说神经递质分泌、信号转导和基因表达等。

We observed that a majority of dsRNA was localized in the nuclear periphery discontinuously after 24 hours of transfection. Time course showed that the labeled dsRNAs were maintained in cultured silkworm cells for up to 5 days. After six days, the signal was too weak to be detected.

用荧光显微镜观察FAM标记的dsRNA在细胞内的定位，发现在转染24小时后，dsRNA开始越来越多的聚集在细胞核的周围，并呈不连续分布，细胞内的荧光强度也越来越强。dsRNA在家蚕细胞中可以维持5天的时间，但到第6天时细胞内已检测不出荧光信号。

Preparation of ratiometric pH nanosensors and its appication in intracellular pH detection of the anti-tumor drugs treated Hela cells The ratiometric pH nanosensors that contained a pH sensitive indicator fluorescein isothiocyanate and a reference dye tris(2, 2′-bipyidyl) dichlororuthenium hexahydrate had been prepared with O/W microemulsion method.

利用该内参比pH纳米传感器尺寸小可以通过细胞的内吞而无损伤地进入细胞内的优势，进一步结合流式细胞学分析方法，对不同抗肿瘤药物作用的Hela细胞内的pH变化进行了分析，结果表明：不同抗肿瘤药物作用后的Hela细胞内pH的变化情况并不一样，有的作用后引起了细胞酸化，有的作用后引起了细胞碱化，有的作用后基本上不引起变化。

AIM: To observe the effect of solanine on the contents of caspase-3 and Bcl-2 in HepG2, and to explicate the mechanism by which solanine induces the apoptosis of tumor cells. METHODS: Laser confocal scanning microscopy and Western blot were used to measure the contents of caspase-3 and Bcl-2, and LCSM was used to determine their locations in the cell. RESULT: Solanine markedly increased the content of caspase-3 while decreasing that of Bcl-2 in HepG2, both in a dose-dependent way.

目的：观察龙葵碱对HepG2细胞内caspase-3及Bcl-2蛋白含量的影响，阐明龙葵碱诱导肿瘤细胞凋亡的作用机制方法：采用激光共聚焦扫描显微术和Western blot法检测caspase-3和Bcl-2蛋白含量，并对二者在细胞内的位置进行定位结果：龙葵碱能够显著升高跳西2细胞内caspase-3蛋白含量，降低Bcl-2的含量，并且均具有剂量依赖性。

Then we transfected transitorily the recombinant of green fluorescent protein gene and middle molecular weight neurofilament cDNA into wide type N2a (N2a/wt) and N2a/tau40 to observe the effect of tau accumulation on GFP-NFM fusion protein transport in cellular processes in living cells. At last we used an apoptotic inducer, camptothecin (an inhibitor of topoisomerase-1) to treat N2a/wt and N2a/tau40 cell lines, and compared their apoptotic response.

主要结果如下：一、tau蛋白过度表达和聚积对细胞形态的影响：倒置显微镜下观察两种细胞的形态，发现N2a/wt细胞的突起多而长，而N2a/tau40细胞胞体变圆，突起明显缩短；免疫印迹结果显示转染了tau40的细胞内tau的免疫反应约增加14倍，免疫荧光结果显示N2a/tau40细胞胞体内呈现出较强的红色荧光，tau主要分布在核周和突起起始部分的胞质内，而N2a/wt细胞内的荧光很弱。

Hen THP-1 macrophages were induced to the foam cells by ox-LDL. Treatment of THP-1 macrophage derived foam cells with apoA-Ⅰ, forskolin (FRK, an adenyl cyclase activator), and SQ-22536 for long periods of time (24 h). In addition, THP-1 macrophage derived foam cells were treated with increasing amounts of FRK (0, 10, 20, 40 and 80 μmol/L) and treated with FRK for increasing time(0, 6, 12 and 24 h).

HP-1巨噬细胞源性泡沫细胞经各种因素处理后，采用油红&O&染色，观察细胞内的脂滴，运用液体闪烁计数器检测细胞内胆固醇流出，高效液相色谱分析细胞内总胆固醇、游离胆固醇和胆固醇酯含量，用逆转录-聚合酶链反应和蛋白质印迹分析法分别检测ABCA1 mRNA与ABCA1蛋白质的水平。

But, there was no Ca(superscript 2+) precipitate in tapetal cells of Zhenshan 97B at the pollen mother cell stage and the meiosis stage and the Ca(superscript 2+) precipitates in tapetal cells mainly deposited in the disaggregated cytoplasm.

而保持系绒毡层细胞遮花粉母细胞时期和减数分裂时期细胞内没有Ca(上标 2+)沉淀；单核花粉时期绒毡层细胞内的Ca(上标 2+)沉淀主要分布在解体的细胞质内。

To test the hypothesis, neontal rat cadiomyocytes Treat with heat shock(HS,42℃,2h) to induce the expression of HSP70,then teated with 0.5mmol/L H2O2. We first found H2O2 can reduced cadiomyocytes apoptosis and HSP70 only over-expression in the HS group, other two group only express HSP70 a little in the test of expression of HSP70.Then we tested the concentration of Ca2+, the [Ca2+ ] of CON was 192.224+6.654, the [Ca2+ ] of H2O2 group was 290.6918+8.922and the [Ca2+]of HS group was 214.2633+4.484.To further determine how HSP70 repaired the Ca2+ homestasis, we measured calcium transient of each group.

实验中应用MTT和流式细胞技术检测细胞的凋亡，发现损伤组的存活率明显低于其他2组，热休克组(Heat shock group，HS group)细胞存活率略低于正常组细胞；免疫组化结果表明只有在HS组才检测到阳性反应，即是HSP70的表达，其他2组均为弱阳性结果；应用离子成像技术对细胞内钙离子浓度进行测定，发现损伤组290.6918+8.922明显高于正常组192.224+6.654和HS组214.2633+4.484，HS组细胞内的钙离子浓度略高于正常组；为了进一步探讨HSP70对于ROS引起的心肌凋亡过程中钙离子的调控机制，又对各组的心肌细胞进行了钙瞬变的测定。

Firstly, the acute cytotoxicity of profenofos on FG-9307 cells was studied, it was found that the cell growth rate was markedly reduced by profenofos at all the concentrations tested; and that the fine structures of the cells were also altered by profenofos, as evidenced by dilation of nuclear membranes and mitochondria cristae, and presence of enlarged lysosomes with engulfed organelles and numerous vacuoles in the cytoplasm.

丙溴磷对FG-9307的急性细胞毒性研究结果发现：1）在所有实验浓度下，细胞的生长速度均受到抑制。2）细胞的超微结构被丙溴磷所改变，最明显的特征则是线粒体损伤严重，嵴肿胀，部分溶解破裂；核膜肿胀，有溶解；细胞内的电子密度高的溶酶体增多；内质网的膜溶解，核糖体从粗面内质网上脱落下来，游离于胞质中。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。