细胞

Hyperthermia was applied to the latter,41.5 degrees Centi grade, 1 h, after having received same irradiation. The cel surviving fractions were determined by clonogenic assay and the parameters of the multi-target/single-hit model, Do, D9, as well as those of the linear-quadratic model, ratio of alpha and beta, were elicited.

集落形成率实验检测两组细胞在不同情况下的细胞存活分数，分别根据多靶单击模型和线形二次模型拟合绘制细胞存活曲线，求出 D0、Dq及α/β比等细胞存活曲线参数，并获得照射2 Gy时的存活分数SF2。

Serum free medium containing 0,16,32,64,128/μg/mL was added 24 hours after culture and cultured for another 24 hours. Cells were collected centrifugally and resuspended in 75% alcohol. Finally, cells were fixed 4℃overnight. Then cells were stained with PI for 30 min under 4℃.. FCM was run with parameter FL2 - A.

流式细胞仪检测细胞凋亡率对数生长期的BIU-87细胞接种于培养瓶内，24 h后更换含有终浓度0、16、32、64、12μg/mL的无血清培养液，处理24 h，离心收集，75%冷乙醇悬浮后4℃固定过夜，PI染液4℃避光染色30min，流式细胞仪于FL2-A参数下检测。

The characteristical time-dependent expression of transcriptional factors HNF4α, C/EBPα, and C/EBPβ during the hepatocyte differentiation by bone marrow stem cells demonstrates that the expressions of these transcriptional factors are closely related to the initiation and maintenance of hepatocyte differentiation.

结论在肝细胞分化过程中，转录因子HNF4α、C/EBPα和C/EBPβ呈特征性时序表达，表明骨髓间充质干细胞在向肝细胞分化时，肝细胞相关转录因子的表达与细胞分化的启动和维持密切相关。

Methods we build rat retinal cells and rgcs cultured in virto by high pressure.put 80 mmhg pressure to rgcs in close tightly culture bottle for four hours, then collect rgcs and check the sum of apoptosis cells by flow cytometry.put the pressure 80 mmhg to rgcs in close tightly culture bottle for four hours,then collect rgcs and look for the apoptotic gene by gene chip.

（1）在rgcs中加入dsx，加压培养后收集细胞，用流式细胞仪测定凋亡细胞百分率。（2）在rgcs中加入dsx，加压培养后收集细胞，用基因芯片寻找凋亡基因的表达。结果（1）在rgcs高压培养时，dsx组的凋亡百分率低于模型组和对照组。

Part Two: The effect of fruit extract on CRL-2606 production of PGE2 Effect of anthocyanin rich fruit extracts on endothelial cells'PGE2 production Epidemical experiment shows that phenolics in fruits and vegetables may prevent the occurrence of chronicle cardiovascular heart disease. To investigate the activity of fruit extracts on artery blood vessel cells, we chose CRL-2606 cell line, a normal iliac artery endothelia cells. Using indomethacin as control, we investigate the effect of blueberry extracts, blackcurrant extracts, chokeberry extracts, cyanidin3-glucoside, and malvidin3-glucoside on the PGE2 production of CRL-2606 cells.

第二部分水果提取物对人体细胞系CRL-2606产生PGE2的影响水果提取物对血管内皮细胞释放PGE2的影响流行病学调查表明，蔬菜水果中的多酚类物质具有预防慢性心血管疾病的作用，为研究水果提取物对血管细胞的作用，我们选择回肠动脉内皮细胞CRL-2606细胞系作为研究对象，以indomethacin为对照，研究了blueberry，blackcurrant，chokeberry提取物及cyanidin3-glucoside，和malvidin3-glucoside对血管内皮细胞释放PGE2的影响。

Results showed that TDI induced toxic action on spermatogenic cell,while the chondriosome was its target within the length and concentrations.

结果表明：在本实验染毒时间和染毒剂量范围内，TD I对雄性的生殖毒性主要表现为对各级生精细胞均有一定的毒性作用，作用的靶点是细胞内的线粒体；TD I通过影响各种酶的活性，干扰了睾丸组织的有氧代谢和无氧供能，抑制了细胞对能量的利用，损伤了各级生精细胞，导致生精上皮不可逆的损害，从而造成了其对雄性生殖系统的损伤；TD I对雄性生殖细胞的核酸代谢以及DNA合成有一定影响。

The chondriosome signal transduction pathway: In the existing of dATP, Cytc combines with Apaf-1, to exposure CARD motif on Apaf-1, and then combines CARD of proaspase-9 into apoptosome, The result is the activation of procaspase-9, and the latter activates Caspase-3, 6s, according induces apoptosis.

已有研究表明，某些细胞在其发生发展过程中Fas的表达不断增加，而凋亡抑制基因Bcl-2的表达逐渐减少，最终导致细胞凋亡的发生，完成细胞代谢过程，达到细胞增殖与死亡的平衡。

Results indicated that the variation was significant from treatment to treatment. S-deficiency caused bulging chloroplasts, loosening and dishevelled grana lamella, reducing organelle, and damaged chondriosome in mesophyll cells of the leaves, and empty cells of the roots, while S-excess, however, led to densification, condensation and randomization of grana lamella of the chloroplasts of the leaves, and separation of cytoplasms from walls of the cells of the roots.

结果表明，受S素营养胁迫的水稻，其根系及叶片的超微结构均发生了显著的变化：低S胁迫会造成水稻叶片叶肉细胞内的叶绿体结构肿胀，基粒片层松驰、散乱，细胞器减少，线粒体结构被破坏，根系细胞内几乎没有内含物；S浓度高则会使叶片叶绿体基粒片层致密、浓缩，无规则化，根系细胞发生质壁分离。

At protein level, AQP8 is expressed in placental syncytiotrophoblasts, amniotic epithelial cells, and chorion cytotrophoblasts.

在蛋白质水平显示，水通道蛋白8表达于胎盘的合体滋养细胞、羊膜上皮细胞及平滑绒毛膜细胞滋养细胞。

Immunohistochemistry results revealed that AQP8 protein was expressed in placental syncytiotrophoblasts, amniotic epithelial cells, and chorion cytotrophoblasts.

免疫组织化学结果显示，水通道蛋白8表达于胎盘合体滋养细胞、羊膜上皮细胞及平滑绒毛膜细胞滋养细胞。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。