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GFP-labeled and non-labeled cells from X blastoderm were large and round with the same characters of morphology. The GFP protein was not expressed in CEF cell .Our results suggested that the CVH promoter regions have specific activity in stage X blastoderm.

实验结果显示:在胚盘细胞转染后12 h可观测到绿色荧光表达,转染后24 h荧光细胞增多,细胞形态均呈圆形、体积较大;在鸡胚成纤维细胞未检测到GFP表达。

Routine detecting techniquefor cell immunity such as white blood count and differential, lymphocyte blastogenesis test has beenused, and combine with flow cell technology to detect the level of cell immunization after advancedimmunization at several important aspect, and tracking experiments to observe changes in the numberof the WBC and lymphocytes, lymphocyte proliferation, the dynamic changes of CD4~+ and CD8~+lymphocyte subsets andγ-IFN; and detected dynamic changes rules of IgG, IgM in humor andmucosa with indirect ELISA, then compare with ordinary immunization methods.

主要采用白细胞计数和分类计数、淋巴细胞转化试验等常规细胞免疫检测技术结合先进的流式细胞技术从细胞免疫的几个重要方面入手检测猪瘟超前免疫后细胞免疫的水平,并进行跟踪试验,观察其白细胞、淋巴细胞数目变化情况,淋巴细胞增殖情况,CD~(4+)和CD~(8+)淋巴细胞亚群及γ-IFN的动态变化规律;同时通过间接ELISA方法检测体液和黏膜中各IgG、IgM的动态变化规律,同时与普通免疫方法进行比较。

Methods MTT assay was used to determine the proliferation inhibition by cantharidin to A549 cell. FACS can were applied to detect the change of cell cycle.

采用MTT法检测斑蝥素对A549细胞增殖的抑制作用;流式细胞仪分析A549细胞周期及斑蝥素对细胞周期的影响。

Mesenchymal stem cells can divide into various cell types, such as ossification cell[13], fat cell[14], cartilage cell[15, 16] etc..

间充质干细胞可分化成多种细胞类型,如成骨细胞[13],脂肪细胞[14],软骨细胞[15,16]等。

It is a complex process involving cell division, differentiation and interaction between cells in the microenvironment of the seminiferous tubule.

睾丸生精小管中的生精细胞先后经过三个阶段的变化:精原细胞的增殖,精母细胞的减数分裂和精子细胞的变态过程,最终产生成熟的精子。

Growth of cells were observed daily by MTT assay, the status of cell proliferation were observed by Plate colony formation, and the cell cycle of the cells were observed with flow cytometry .

应用MTT比色法绘制细胞生长曲线;克隆形成实验了解细胞增殖情况;流式细胞仪观察细胞周期。

The effects of osmosis properties of the cell wall on the mechanical response of the cell were discussed.

在建立植物细胞力学模型的基础上,利用膜理论推导出细胞在压缩载荷作用下的平衡方程,通过数值仿真的方法得到细胞的力学响应,分析了细胞壁的渗透作用对细胞力学响应的影响。

Cytomorph classification of light microscope:21 cases are spindle cell , 12 cases are cellula epithelialis and 5 cases are mixed cellularity.

光镜下细胞形态分类:梭形细胞为主型21例,上皮细胞为主型12例,混合细胞型5例。

The cytomorph classification of light microscope:21 cases are spindle cell , 12 cases are cellula epithelialis and 5 cases are mixed cellularity.

光镜下细胞形态分类:梭形细胞为主型21例,上皮细胞为主型12例,混合细胞型5例。

Bone morphogenetic protein -2 induces the expression of cementum attachment protein in human periodontal ligament clones [J].

本研究通过比较骨髓基质细胞与正常人牙周韧带细胞表面抗原的表达情况,证实牙周韧带细胞与骨髓基质细胞在表面抗原的表达上相似。

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